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Application of 10-gingerol to preparation of medicines for preventing and treating epizoic ciliates of fishes

The technology of ciliate and gingerol is applied in the application field of 10-gingerol in the preparation of medicaments for preventing and controlling ectoparasitic ciliates in fish, which can solve the problems of polluting the environment, high toxicity and high residue, endangering consumers' health and the like, and achieves the effect. Good, no environmental pollution and residues, the effect of broad prospects for industrialization

Inactive Publication Date: 2016-01-27
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high toxicity and high residue of these drugs, which pollute the environment and endanger the health of consumers, they have been banned from use.

Method used

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  • Application of 10-gingerol to preparation of medicines for preventing and treating epizoic ciliates of fishes
  • Application of 10-gingerol to preparation of medicines for preventing and treating epizoic ciliates of fishes
  • Application of 10-gingerol to preparation of medicines for preventing and treating epizoic ciliates of fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 110

[0020] Example 110-The killing effect of gingerol on adults and cysts of the multiseed melon worm

[0021] 1.1 Experimental materials: The host model fish is grass carp (Ctenopharyngodonidellus), weighing 25.7±3.8g, which comes from the fish fry farm of Wuxing Village, Huadu District, Guangzhou City. Ichthyophthirius multifiliis comes from the flower, bird, fish and insect market in Huadiwan, Guangzhou. The purity of 10-gingerol used in the experiment is ≥98%, which was prepared and provided by the Institute of Hydrobiology of Jinan University. The specific preparation method is as follows:

[0022] 2 kg of ginger oleoresin extracted from ginger was purchased from Guizhou Hongxing Development Duyun Lvyou Co., Ltd. It was sequentially extracted with petroleum ether, ethyl acetate, and water to obtain 500 g of petroleum ether extract, 1200 g of ethyl acetate extract, and 300 g of water extract. Through the insecticidal experiment, it was determined that the ethyl acetate part...

Embodiment 210

[0033] The killing effect of embodiment 210-gingerol to the larvae of the multi-spun melon worm

[0034] 2.1 Experimental materials: the same as in Example 1.

[0035] 2.2 Experimental method:

[0036] 2.2.11 Preparation of 110-gingerol drug: Same as Example 1.

[0037] 2.2.2 Add 100 μL of aqueous solution containing about 200 multisporidia larvae to each well of a 96-well plate, and add another 100 μL of liquid medicine, so that the final concentrations of 10-gingerol in each well are 32, 16, 8, 4, 2, 1, 0.5 and 0 (control) mg / L, each set 6 parallel. Under the microscope, count the death time of larvae within 4h and the mortality rate of larvae in the 4th hour, and calculate the EC 50 ,Calculated as follows:

[0038] Mortality rate (%)=(1-experimental group live larva number / control group larva total number) * 100%;

[0039] The results showed that 10-gingerol could kill 100% of the larvae within 4 hours at the concentration of 2mg / L. And as the concentration increases,...

Embodiment 3

[0040]Embodiment 3 The influence of low concentration 10-gingerol on larva infectivity

[0041] 3.1 Experimental materials: the same as in Example 1.

[0042] 3.2 Experimental method:

[0043] 3.2.11 Preparation of 110-gingerol medicinal solution: According to the method of Example 1, 120 mL of medicinal solutions of 2, 1, 0.5, 0.25 and 0 (control) mg / L were prepared.

[0044] 3.2.2 Adjust the concentration of larvae with aerated distilled water, take 40mL containing about 2×10 5 Add an aqueous solution of infectious larvae into a 100mL beaker, then add 40mL of liquid medicine, mix well, and treat the infectious larvae in 10-gingerol test solution with final concentrations of 1, 0.5, 0.25, 0.125 and 0 mg / L for 2 hours , each concentration set 3 treatments in parallel.

[0045] 150 healthy grass carp that were not infected with the multi-seed melon worm were randomly and evenly distributed to 15 aquariums (10 fish in each aquarium, and 5L of aerated tap water was added in ad...

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Abstract

The invention discloses application of 10-gingerol to preparation of medicines for preventing and treating epizoic ciliates of fishes and belongs to the technical field of fishery medicines. The application has the advantages that the 10-gingerol is applied to preparation of the medicines for preventing and treating the epizoic ciliates of the fishes firstly, is particularly used for killing ichthyophthirius multifilis and is capable of killing 100% of ichthyophthirius multifilis within an effective dose range so as to play a role in effective prevention and treatment of the ichthyophthirius multifilis; zingiber officinale, the source of the 10-gingerol, is perennial herb of zingiberaceae, is an Asian traditional medical and edible dual purpose plant and is safe and free from environmental residues; LC50 (lethal concentration 50) of the 10-gingerol for ctenopharyngodon idellus is 85.43 mg / L which is 21.4 times of minimum effective concentration (4 mg / L) that the 10-gingerol kills the Ichthyophthirius multifilis, and accordingly the 10-gingerol is high in use safety; the zingiber officinale is high in 10-gingerol content, 10-gingerol extraction and preparation technologies are simple and mature, and the 10-gingerol is high in ciliate resisting activity and free from environmental pollution and residues, thereby being promising in application prospect on the field of novel environment-friendly fishery medicines.

Description

technical field [0001] The invention belongs to the technical field of fishery medicines, and in particular relates to the application of 10-gingerol in the preparation of medicines for preventing and treating fish ectoparasitic ciliates. Background technique [0002] Fish ectoparasitic ciliates are serious diseases that endanger the freshwater aquaculture industry. As a kind of fish ectoparasitic ciliate, it has no selectivity for the host, and can infect various freshwater and brackish water fish such as grass carp, freshwater pomfret and channel catfish. The worm mainly parasitizes on the gills, fins and skin of fish, causing epithelial cells to proliferate and many small white spots appear. Therefore, small melon worm disease is also called "white spot disease". The disease is widely distributed and spread all over the world. From April to June and from September to November in my country every year, the water temperature is 20°C to 24°C, which is the peak period of the...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P33/14
Inventor 张其中王彬
Owner JINAN UNIVERSITY
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