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A Semen Dilution Method Applicable to Artificial Insemination in Breeder Farms

A breeder farm and semen technology, applied in the field of poultry breeding, can solve the problems of reducing equipment and labor costs, cumbersome quantitative dilution operations, etc., and achieve the effects of accurate prediction, improved vitality, and extended shelf life

Active Publication Date: 2017-01-04
湖南银孜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the above-mentioned problems and defects existing in the artificial insemination technology of chicken diluted semen, the present invention provides a semen dilution method that can be used for artificial insemination in breeder farms. The method includes the preparation, packaging and storage of dilute chicken semen Technology, as well as system technologies such as diluent thawing, carrying, preheating, quantitative addition, mixing, insemination and other system technologies that are matched with the existing industrialized insemination technology. First, a certain volume and dilution ratio of a cup of semen collected during insemination are determined. The volume of each diluent can be divided into one cup of semen and one diluent, which solves the cumbersome problem of quantitative dilution operation in production. Secondly, the diluent is preheated by body temperature heating, which simplifies the need for constant temperature water bath heating before diluent in the prior art. cumbersome operation problems, and relatively accurate isothermal dilution can be achieved; at the same time, through the combination of film, hand and sperm collection tube, the integration of sperm collection, dilution and mixing can be realized ingeniously without changing the current working status to the greatest extent. It is completed by human beings, which reduces the technical difficulty and reduces the cost of equipment and labor

Method used

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  • A Semen Dilution Method Applicable to Artificial Insemination in Breeder Farms
  • A Semen Dilution Method Applicable to Artificial Insemination in Breeder Farms
  • A Semen Dilution Method Applicable to Artificial Insemination in Breeder Farms

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The specific operation method includes the following steps:

[0037] 1) Preparation of diluent and cryopreservation: Take 7.0g of fructose, 6.0g of sodium glutamate, 11.0g of dipotassium hydrogen phosphate, 0.7g of potassium dihydrogen phosphate, and N-trimethylolmethyl-2-aminoethanesulfonic acid 3.0g, potassium citrate 0.8g, sodium acetate trihydrate 3.0g, anhydrous magnesium chloride 0.4g, lycopene 0.05g, add quartz double distilled water to 1.0L; the prepared chicken semen dilution was quantified in a plastic centrifuge tube 5mL Aliquot and store in freezer at -18°C for later use;

[0038] 2) Preheat chicken semen dilution: take the dilution out of the freezing environment and store it in a miniature thermos cup that can be carried with you. A small amount of ice cubes are placed in the miniature thermos cup, which can meet the temperature requirement of 6-10°C. Put it in the breast pocket of the skin-fitting jacket for the first 30 minutes to preheat it until the t...

Embodiment 2

[0045] The specific operation method includes the following steps:

[0046] 1) Preparation of diluent and cryopreservation: Take 9.0g of fructose, 4.0g of sodium glutamate, 14.0g of dipotassium hydrogen phosphate, 0.5g of potassium dihydrogen phosphate, and N-trimethylolmethyl-2-aminoethanesulfonic acid 5.0g, potassium citrate 0.4g, sodium acetate trihydrate 6.0g, anhydrous magnesium chloride 0.2g, lycopene 0.1g, add quartz double distilled water to 1.0L; the prepared diluent of chicken semen is quantified with 1mL diluent bottle Aliquot and store in freezer at -20°C for later use;

[0047] 2) Preheat chicken semen diluent: take the diluent out of the frozen environment and store it in an environment of 8°C, put it in a skin-fitting trouser pocket 20 minutes before insemination, and preheat it until the temperature difference with the chicken semen is within ±3°C Inside;

[0048] 3) Dilution and mixing: directly use the preheated diluent bottle containing 1mL volume diluent ...

Embodiment 3

[0051] The specific operation method includes the following steps:

[0052] 1) Preparation of diluent and cryopreservation: Take 8.0g fructose, 5.0g sodium glutamate, 12.0g dipotassium hydrogen phosphate, 0.6g potassium dihydrogen phosphate, N-trimethylolmethyl-2-aminoethanesulfonic acid 4.0g, potassium citrate 0.6g, sodium acetate trihydrate 5.0g, anhydrous magnesium chloride 0.3g, lycopene 0.075g, add quartz double distilled water to 1.0L; use the diluent bottle 4mL quantitatively to dilute the prepared chicken semen Aliquot and store in freezer at -20°C for later use;

[0053] 2) Preheat chicken semen diluent: Take the diluent out of the freezer and store it in a miniature thermos cup that can be carried with you. A small amount of ice cubes are placed in the miniature thermos cup, which can meet the temperature requirement of 6-10°C. In the first 25 minutes, preheat the skin-fitting trouser pocket or the breast pocket of the jacket until the temperature difference with th...

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Abstract

The invention discloses a semen diluting method for artificial insemination in a breeding chicken farm, wherein the semen diluting method comprises chicken semen diluent preparing, sub-packaging and preserving technologies, as well as such systematic technologies matched with an existing industrial insemination technology as diluent unfreezing, carrying, preheating, quantitative adding, uniformly mixing, inseminating and the like. The method, which preheats a diluent in a mode of body temperature heating, not only solves the problem of the prior art that is complex to heat in a water bath at constant temperature before using the diluent but also achieves relatively precise isothermal dilution; the volume of a tube of the diluent is determined according to the volume and the dilution rate of a cup of semen which is collected during inseminating, so that one tube of the diluent corresponds to one cup of the semen and the problem of a complex operation of quantitative dilution in production is solved; and meanwhile, the method, which combines a thin film, a hand and a semen collector, skillfully achieves the integration of semen collection, dilution and uniformly mixing by one person without changing an existing working condition to the greatest extent, so that technical difficulties are reduced.

Description

technical field [0001] The invention belongs to the field of poultry breeding, and relates to poultry semen dilution and artificial insemination technology, in particular to a semen dilution method that can be used for artificial insemination in breeder farms. Background technique [0002] With the development of the modern poultry industry, the scale and intensification of poultry breeding are getting higher and higher. In order to increase the fertilization rate, accelerate the process of improved breeds, improve the utilization rate of excellent breeders, and reduce the number of breeding roosters, etc., breeding Chicken artificial insemination technology is becoming more and more important as an indispensable means in modern breeding and production. At present, almost all chicken semen is used for artificial insemination. On the one hand, if the undiluted chicken semen is stored for more than half an hour, the fertilization rate will be affected; on the other hand, the u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D19/02
CPCA61D19/02
Inventor 燕海峰张佰忠易康乐孙鏖张晓华朱立军李昊帮李晟王庆桥
Owner 湖南银孜科技有限公司
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