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Application of squillid polypeptide in resisting lung cancer

A technology for mantis shrimp and lung cancer, which is applied in antitumor drugs, hydrolyzed protein components, fermentation, etc., can solve the problems of large toxic and side effects, poor selectivity, etc., and achieves the effects of mild extraction method, rich nutrition and significant proliferation inhibition effect.

Inactive Publication Date: 2016-02-03
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Malignant tumors are a type of disease that seriously threatens human health and life, and the incidence rate is getting higher and higher. Although the existing chemotherapy drugs have certain curative effects on most tumors, they have poor selectivity, large toxic and side effects, and drug resistance. The problem is still very obvious

Method used

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  • Application of squillid polypeptide in resisting lung cancer
  • Application of squillid polypeptide in resisting lung cancer
  • Application of squillid polypeptide in resisting lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Preparation of mantis shrimp polypeptide

[0020] (1) Enzymatic hydrolysis process

[0021] Use trypsin to hydrolyze the mantis shrimp soft tissue: wash and remove the mantis shrimp, add the mantis shrimp meat to distilled water with a mass ratio of 1:3 for homogenization, adjust the pH value of the solution with 0.1mol / L HCl and 0.5mol / L NaOH, Add trypsin for heat preservation and hydrolysis. After the hydrolysis is completed, inactivate the enzyme at 100°C for 10 minutes, centrifuge the hydrolyzate at 8000r / min for 20 minutes, take the supernatant, rotary evaporate, and freeze-dry.

[0022] (2) Influencing factors and optimization of enzymatic hydrolysis process

[0023] According to the above process steps, observe the influence of factors such as pH value, enzyme amount, temperature and enzymolysis time on the enzymolysis process, and compare the anti-tumor activity of the enzymatic polypeptide under different hydrolysis conditions, so as to determine...

Embodiment 2

[0037] Example 2: Mantis shrimp polypeptide inhibits the proliferation of lung cancer H1299 cells

[0038] (2.1) Cell culture

[0039] Human lung cancer cells H1299 (originally purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences and preserved by our laboratory) were inoculated into RPMI1640 medium containing 10% fetal bovine serum and double antibodies, and incubated at 37°C in 5% CO 2 Cultured in an incubator, the cells grow in a single layer adherent, digested with 0.25% trypsin, and passaged once every 2 to 3 days, and the cells in the logarithmic growth phase are used in the experiment.

[0040] (2.1.1) The effect of different concentrations of mantis shrimp polypeptide on the proliferation of lung cancer H1299 cells

[0041]The mantis shrimp polypeptides obtained in Example 1 were configured to be 5.0mg / ml, 10.0mg / ml, 15.0mg / ml, 20.0mg / ml and 25.0mg / ml respectively, and the MTT method in Example 1 was used to detect the concentration of mantis shrimp...

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Abstract

The invention relates to application of squillid polypeptide in resisting lung cancer, in particular to application of squillid polypeptide in drug for inhibiting lung cancer H1299 cell proliferation. The application includes that squillid is used as a raw material, trypsin is utilized to enzymolyze squillid meat to obtain squillid polypeptide, and enzymolysis process conditions are controlled to enable squillid polypeptide extracted by enzymolysis to inhibit proliferation activity of lung cancer H1299. Compared with the prior art, an extraction method of squillid polypeptide is mild, process is simple, squillid polypeptide extracted has remarkable effect on inhibiting proliferation of the lung cancer H1299 cells cultured in vitro, and a rate of inhibiting proliferation of the H1299 cells reaches more than 40.3% when concentration is 5-25mg / ml.

Description

technical field [0001] The invention relates to the application of mantis shrimp enzymatic hydrolyzate, in particular to the application of mantis shrimp enzymatic hydrolyzate polypeptide in anti-lung cancer. Background technique [0002] Mantis shrimp (Oratosquillaoratoria) belongs to the phylum Arthropoda, Crustacea, Molecula, Stomatopoda, Mantisidae, and the genus Oratosquilaoratoria. Distributed in coastal areas of China, Japan, Korea and Southeast Asia. Both the north and south coasts of my country are produced in large quantities, and the output is very large. The mantis shrimp is delicious, nutritious, and cheap, and is very popular among consumers. The current research on mantis shrimp mainly includes: the ethanol extract of mantis shrimp can inhibit the growth of CNE-2Z cell transplanted tumors in nude mice, reduce the invasion ability of CNE-2Z cells in vitro, and the histochemical localization of NADPH2d in the nervous system of mantis shrimp Research, etc. Most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01C12P21/06A61P35/00
Inventor 马剑茵李云涛卜天
Owner ZHEJIANG OCEAN UNIV
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