Penicillin sodium degrading bacterium PC-2 and application thereof

A penicillin degradation, penicillin sodium technology, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve the problems of pollution, difficult to effectively decompose antibiotics, and inefficient utilization of penicillin residue, so as to improve economic efficiency and reduce environmental pollution Effect

Inactive Publication Date: 2016-02-03
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] In order to overcome the problems that antibiotics in antibiotic residues are difficult to effectively decompose, resulting in the inefficient utilization of penicillin residues and a

Method used

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  • Penicillin sodium degrading bacterium PC-2 and application thereof
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  • Penicillin sodium degrading bacterium PC-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening and Identification of Penicillin Sodium Degrading Bacteria PC-2

[0046] (1) Isolation and screening of penicillin sodium degrading bacteria PC-2

[0047] Get the mixed compost sample of 10g penicillin slag (Hebei Pharmaceutical, Shijiazhuang, Hebei) and pig manure (Hengxi Town, Nanjing City, Jiangsu Province) (the composting place is the archway of Nanjing Agricultural University, Nanjing City, Jiangsu Province), and the mass ratio of bacterium slag and pig manure is 1:2), add to a 250mL Erlenmeyer flask containing 90mL of distilled water, shake on a shaker at 37°C and 150r / min to fully mix the sample with water. When the mixture was diluted to a concentration of about 0.1-1.0 ppm by gradient dilution method, 200 μL of samples were pipetted with a pipette gun and spread on LB solid medium with 1000 ppm of penicillin sodium, and incubated at 37 °C for 2 days. After the growth of colonies in the culture medium, pick a single colony, streak and culture for abou...

Embodiment 2

[0054] Degradation Characteristics of Penicillin Sodium Degrading Bacteria PC-2

[0055] (1) Drawing of penicillin sodium solution standard curve

[0056] The concentration of penicillin sodium was quantitatively determined by ultra-high performance liquid chromatography (Waters2695, USA). Chromatographic conditions: mobile phase: 0.1% formic acid aqueous solution-acetonitrile (V:V=50:50); flow rate: 1mL / min; detector is PDA ultraviolet detector; wavelength range 190nm~400nm; column temperature: 30°C; The sample volume is 20μL; the peak time is set to 6min.

[0057] Prepare penicillin sodium working solutions with concentrations of 0.5ppm, 1ppm, 2ppm, 4ppm, and 8ppm, respectively, and then accurately draw 1mL of the standard working solution and inject samples under the set chromatographic conditions. The mass concentration of penicillin sodium solution is the abscissa , draw the standard curve with its peak area as the ordinate (such as Figure 5 shown).

[0058] (2) Dete...

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Abstract

The invention belongs to the field of biodegradation treatment, and provides a penicillin sodium degrading bacterium, separation and identification of the degrading bacterium and application to penicillin sodium degradation of the degrading bacterium. The penicillin sodium degrading bacterium belongs to chelatococcus, the classified name is Chelatococcus sp., the preservation serial number is CGMCC No.1.15283, and the penicillin sodium degrading bacterium is preserved in China microbial strain preservation management council common microorganism center on June 11th, 2015. Degradation characteristic research shows that through 12-d shaking culture at the temperature of 37 DEG C on the condition of 150 r/min, the degradation rate of PC-2 to penicillin sodium with the initial concentration of 200 ppm is 53.5%. When glucose serves as a carbon source, peptone serves as a nitrogen source, the degrading bacterium inoculum size is 14%, the initial concentration of penicillin sodium is 100 ppm, and the pH value range is 6.0-7.0, the degradation rate is the largest. The penicillin sodium degrading bacterium has a good degradation characteristic to penicillin sodium, and provides a certain reference basis for degrading penicillin residual through a biological method.

Description

technical field [0001] The invention belongs to the field of biodegradation treatment, and relates to a penicillin sodium degrading bacterium, its isolation and identification, and the degradation application of the penicillin sodium degrading bacterium. Background technique [0002] Penicillin residue is the main waste in my country's antibiotic fermentation industry. According to preliminary estimates, in 2009 China produced about 1.3 million tons of antibiotic residue. In 2002, the Ministry of Agriculture, the Ministry of Health, the State Drug Administration and other departments listed antibiotic residues in the catalog of drugs that are prohibited from being used in feed and animal drinking water; in 2008, the Ministry of Environmental Protection listed antibiotic residues in the " National Catalog of Hazardous Wastes. In view of the practical problems such as the large output of antibiotic residues and the difficulty of treatment, and the "Pharmaceutical Industry Pol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 刘燕张振华赵娟余冉于赐刚董姗姗章嫡妮王长永
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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