Fluorescent optical sheet microscopic imaging system and method

Abstract

The invention discloses a fluorescent optical sheet microscopic imaging system and method. The fluorescent optical sheet microscopic imaging system comprises a detecting device, a correcting device, an imaging device and a controlling device, wherein the detecting device is used for detecting wave-front aberration distortion of all preset isoplanatic regions of a tissue plane imaging view field in a living specimen and transmitting the wave-front aberration distortion to the controlling device, the controlling device is used for giving a correcting instruction to the correcting device according to the received wave-front aberration distortion, the correcting device is used for performing at least one time of wave-front correcting on the isoplanatic regions simultaneously according to the received correcting instruction, and the imaging device is used for imaging the tissue plane processed through wave-front correcting. The fluorescent optical sheet microscopic imaging system is beneficial for obtaining the deep biological tissue plane with the larger view field and the high temporal-spatial resolution.

Description

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AI Technical Summary

Benefits of technology

This technology allows for precise detection of surface waves that can cause image quality issues such as blurring or ghosting during medical imagery analysis. By dividing an area on the sample's tissue plan into multiple areas called isochoric zones, it becomes possible to correct any errors caused by these irregularities while still maintain good accuracy over longer periods of time compared to traditional methods like histograms.

Problems solved by technology

This patented technology solves issues related to conventional optical sectioning techniques such as slow down tomography and inspection underwater. These limitations include reduced brightness during photolysis caused by scattered lasers from excited emitted lights within certain wavelength ranges, difficulty obtaining images over 10-20 millimeters wide without blurriness, poor quality control accuracy, lack of ability to measure dynamic behavior inside living cells, difficulties in achieving both small diameter and long length gratings, and challenges associated with current devices like LSF Micromirror Devices (LFS MODIS)).

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