Cyclamen plant callus and bud culture medium and culture method

A technology of callus and culture method, applied in botany equipment and method, horticultural method, plant regeneration, etc., can solve the problems of character separation, high price, high production cost, etc., achieve browning inhibition, high reproduction coefficient, prevent browning effect

Inactive Publication Date: 2016-02-24
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic high-quality varieties of cyclamen are extremely scarce, and foreign original species are not only expensive, but also because most of them are F 1 Generation, continued breeding wil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Experimental material: the plant parent of the present invention adopts the cyclamen "national flag red" variety, which is purchased from the flower market of Tieji Road, Qingshan District, Wuhan City. Young and healthy leaves of cyclamen were used as explants.

[0037] equipment:

[0038] ⒈ Galanz Microwave Oven, Mingxiu Guangbo G8023SCL-K3, Foshan Shunde Galanz Microwave Electric Co., Ltd.;

[0039] 2. Shuangha automatic stainless steel double-layer vertical electric steam pressure sterilizer, YX.350Z, Shanghai Sanshen Medical Instrument Co., Ltd.;

[0040] ⒊BOXUN clean bench, cleanliness grade 100 (≥0.5μm particles≤3 particles / L) YS-800-2;

[0041] 4.TONE intelligent light incubator, GXZ type, Nanjing Tongli Experimental Instrument Co., Ltd.;

[0042] 5. Electronic balance, 200mL Erlenmeyer flask, square plastic culture bottle with lid, tweezers, scalpel, petri dish, filter paper, alcohol lamp.

[0043] Steps:

[0044] 1. Culture medium preparation

[0045] Confi...

Embodiment 2

[0062] Steps:

[0063] 1. Culture medium preparation

[0064] Configure the substratum of cyclamen plant callus and bud, its component is as follows,

[0065] components

Concentration (mg / L)

NH 4 NO 3

1650

KNO 3

1900

CaCl 2 2H 2 o

440

MgSO 4 ·7H 2 o

370

K H 2 PO 4

170

KI

0.83

h 3 BO 3

6.2

MnSO 4 ·7H 2 o

22.3

ZnSO 4 ·7H 2 o

8.6

Na 2 MnO 4 2H 2 o

0.25

CuSO 4 ·5H 2 o

0.025

CoCl 2 ·6H 2 o

0.025

FeSO 4 ·7H 2 o

27.8

Na 2 ·EDTA·2H 2 o

37.3

Inositol

100

Niacin

0.5

Pyridoxine hydrochloride

0.5

Thiamine hydrochloride

0.1

Glycine

2.0

2,4-D

2

6-BA

0.5

[0066] In addition, 3.0% of sucrose and 0.8% of agar are included in the total mass percentage of the medium, and the pH is adjusted to 5.8-...

Embodiment 3

[0081] Steps:

[0082] 1. Culture medium preparation

[0083] Configure the medium for inducing callus and buds, its components are as follows,

[0084] components

Concentration (mg / L)

NH 4 NO 3

1650

KNO 3

1900

CaCl 2 2H 2 o

440

MgSO 4 ·7H 2 o

370

K H 2 PO 4

170

KI

0.83

h 3 BO 3

6.2

MnSO 4 ·7H 2 o

22.3

ZnSO 4 ·7H 2 o

8.6

Na 2 MnO 4 2H 2 o

0.25

CuSO 4 ·5H 2 o

0.025

CoCl 2 ·6H 2 o

0.025

FeSO 4 ·7H 2 o

27.8

[0085] Na 2 ·EDTA·2H 2 o

37.3

Inositol

100

Niacin

0.5

Pyridoxine hydrochloride

0.5

Thiamine hydrochloride

0.1

Glycine

2.0

NAA

2.5

6-BA

0.2

[0086] In addition, 3.0% of sucrose and 0.8% of agar are included in the total mass percentage of the medium, and the pH is adj...

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PUM

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Abstract

The invention provides a culture medium for inducing cyclamen leaf to form callus and bud and a culture method. Formation of cyclamen callus and bud is highly-efficiently induced by a two-step method, and explant browning is effectively inhibited. The culture method comprises the following steps: before acquisition of explant leaves, cyclamen plant undergoes shading treatment for 24-26 h, and afterwards, in vitro low-temperature (20-22 DEG C) darkness culture is carried out so as to effectively solve the browning problem; then, callus is induced through low-temperature darkness culture, culture temperature is raised to 25-28 DEG C after 30-40 days, and light culture is carried out to induce bud formation; and finally, root induction is conducted after bud has been formed for 15-20 days. The technical system can effectively inhibit browning, has high propagation coefficient, can preserve original traits and heterosis of F1 generation, and provides key technical support for factory production of cyclamen seedlings.

Description

technical field [0001] The invention belongs to the field of plant asexual propagation, in particular to a culture medium and a culture method for inducing cyclamen leaves to form calluses and buds. Background technique [0002] Cyclamenpersicum Mill, also known as Yipinguan, Rabbit Ear Flower, and Radish Begonia, is a perennial bulbous herbaceous flower of Primulaceae Cyclamen. Long, it is one of the top potted flowers in Europe, Japan and other countries, and the market price is expensive. The suitable temperature for the growth of cyclamen is 18-20°C. Cyclamen reproduction methods are divided into sexual reproduction and asexual reproduction. Commodity cyclamen are mostly hybrid generation F 1 , it is difficult to preserve F by seed propagation 1 The original traits and heterosis of the generation, while the seed germination rate is low, the more difficult it is to produce the seeds of varieties with higher ornamental value, which has become the main factor restrictin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 何玉池周雪孙文超王真梅卢翔宇王桂友宋兆建蔡得田
Owner HUBEI UNIV
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