A kind of method of cultivating sterile silkworm cordyceps

A technology of silkworm caterpillar fungus and silkworm eggs, applied in botany equipment and methods, cultivation, fungi, etc., can solve the problems of low content of cordycepin and failure to reach the general level of cordyceps sinensis

Active Publication Date: 2018-10-30
王荫槐 +3
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AI Technical Summary

Problems solved by technology

However, in the existing silkworm fungus culture technology, the obtained cordycepin content is relatively low, which cannot reach the general level of Cordyceps sinensis

Method used

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  • A kind of method of cultivating sterile silkworm cordyceps

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Embodiment 1

[0018] (1) 75% ethanol and 4% formaldehyde solution are soaked and sterilized with the silkworm ovum before the spot blue stage or the spot blue stage;

[0019] (2) placing the silkworm eggs after the disinfection and sterilization in a sterile isolator for hatching, the aseptic isolator includes several horizontally placed rearing trays, and the aseptic isolator keeps the hatching process at Carried out in the dark, the hatching process maintains a constant temperature of 25°C, a humidity of 70-80%, and a transfer rate of aseptically treated air of 250-350 liters per day for the entire sterile isolator , until sterile ant silkworms are obtained;

[0020] (3) Feed the sterile ant silkworms hatched in the aseptic isolator with sterilized artificial feed. Vitamins are obtained, wherein the sterilized artificial feed includes: based on the total weight of the sterilized artificial feed, dry mulberry leaf powder 36.5wt%, defatted soybean powder 35.4wt%, mulberry green branch powd...

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Abstract

The invention provides a method for cultivating sterile silkworm insect grass. The method comprises the steps that silkworm eggs in the head pigmentation stage or before the head pigmentation stage are disinfected and sterilized; the disinfected and sterilized silkworm eggs are placed in a sterile isolator to be hatched; sterile newly-hatched silkworms which are hatched in the sterile isolator are fed with sterile artificial feed; cordyceps militaris bacterium liquid is prepared; the cordyceps militaris bacterium liquid is injected into abdominal cavities of the sterile silkworms of the third to fifth day of five ages; died and stiff sterile silkworms which grow a small amount of hyphae are taken out from a raising plate and then transferred into a sterile environment alternating illumination at 25 DEG C in the daytime with dark at 20 DEG C at night to be cultivated for 38-42 days, the environment humidity is kept 95% or above in the cultivating process, and then the sterile silkworm insect grass of which the whole body completely grows the hyphae and sporophores of different lengths is obtained.

Description

technical field [0001] The invention relates to the cultivation of silkworm fungus, in particular to aseptic cultivation of silkworm fungus. Background technique [0002] Cordyceps sinensis, also known as Cordyceps sinensis, is a precious nourishing medicinal material commonly used by Chinese folks. Its nutritional content is higher than that of ginseng. It can be used as medicine or edible. It is an excellent delicacy with high nutritional value. Because of the cordycepin contained in it, Cordyceps can enhance the body's immunity, nourish the lungs and kidneys, and have obvious inhibitory effects on lung cancer and liver cancer. Clinically, it has curative effects on chronic cough due to lung deficiency, asthma, hemoptysis due to tuberculosis, night sweat, waist and knee pain due to kidney deficiency, impotence and nocturnal emission, neurasthenia, and red blood cell drop after chemotherapy and radiotherapy. The content of cordycepin has become an important indicator of th...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/14A01G18/00A01K67/033
CPCA01G18/00
Inventor王荫槐王琳王世明王梅
Owner王荫槐