Biomechanical model of human brain arteriovenous malformation and its construction method in vitro

An arteriovenous malformation and model technology, applied in the field of cerebrovascular disease experimental research in basic medicine, can solve the problem of difficulty in clarifying the coupling mechanism of vascular endothelial cell force-biochemical signal, inability to vascular biology, and inability to detect the influence of hydrodynamic changes on cytokine expression. and other problems to achieve the effect of repeatable experimental conditions and controllability

Inactive Publication Date: 2017-12-29
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the aid of computer fluid dynamics software, this in vitro model can better simulate the hemodynamic characteristics of malformed blood vessels, but it cannot conduct related research on vascular biological behavior, nor can it detect the effects of hydrodynamic changes on the morphology of malformed blood vessel cells and the expression of cytokines. Impact
Using the above experimental models and methods, it is difficult to clarify the coupling mechanism of vascular endothelial cell force-biochemical signals, and the resulting vascular distortion process
At present, there is no in vitro experimental model that conforms to the vascular architecture characteristics of human cerebral arteriovenous malformation and has its biomechanical characteristics

Method used

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  • Biomechanical model of human brain arteriovenous malformation and its construction method in vitro
  • Biomechanical model of human brain arteriovenous malformation and its construction method in vitro
  • Biomechanical model of human brain arteriovenous malformation and its construction method in vitro

Examples

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Embodiment 1

[0024] Example 1: Using the method of the present invention to observe the influence of hydrodynamic changes on the expression of vascular endothelial growth factor in human cerebral arteriovenous malformation.

[0025] Fresh specimens of surgically resected human brain arteriovenous malformations were rinsed with normal saline, and the adventitia of the malformed blood vessels was stripped off longitudinally, cut into 1.5mm under a microscope 2 Vascular tissue pieces, endothelial side down, stick to the bottom of the culture bottle, place in a 37°C incubator for 10 minutes, and then add M along the wall of the bottle 199 (Gibco BRL) culture medium at 37°C, volume fraction 0.05CO 2 cultured in an incubator. After the endothelial cells adhered to the wall, the D-Hanks solution was used to wash and remove the tissue pieces and non-adhered cells. Under the microscope, the cell brush scraped off the smooth muscle cells and fibroblasts, and the adherent cells continued to be cultu...

Embodiment 2

[0031] Example 2: Using the method of the present invention to observe the influence of hydrodynamic changes on the expression of human brain microvascular endothelial cell growth factor.

[0032] Collect the fresh cerebral cortex around the malformed blood vessels, mechanically grind and filter with microporous nylon mesh, collect the microvascular segments on the net, digest with collagenase, centrifuge and precipitate the microvascular segments, add cell growth medium to dilute, and then plant them in gelatin-covered culture flasks. Place in a 37°C incubator and incubate for 1 hour, and replace the culture medium so as to remove the microvessel segments that fail to adhere to the wall. In order to make the endothelial cells grow well, the primary stage is catalyzed by endothelial cell growth additives.

[0033] Using fibronectin (5.0 μg / cm 2 ) coated the lumen surface of the human brain microvessel model, and after 24 hours, added M to the lumen 199 (Gibco BRL) culture me...

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Abstract

The invention relates to a human brain arteriovenous malformation biomechanics model and an in vitro establishing method thereof. The system comprises a power pump, a gas replacement device, a pressure sensor, a malformation vascular model, a normal bran microvascular model and a liquid storage bottle which are connected in sequence and form a blood feeding artery model circulation loop, wherein the malformation vascular model and the normal bran microvascular model are arranged in parallel. The invention provides the human brain arteriovenous malformation biomechanics model and the in vitro establishing method thereof, which are used for developing research on brain blood vessel fluid dynamics, cytobiology and a vessel malformation mechanism.

Description

technical field [0001] The invention belongs to the field of experimental research on cerebrovascular diseases of basic medicine, and in particular relates to a human brain arteriovenous malformation biomechanical in vitro model and an in vitro construction method thereof. Background technique [0002] Human cerebral arteriovenous malformation is the most common type of vascular malformation in the central nervous system. Its morphological characteristics are direct communication between arteries and veins, and lack of capillary network in the middle. Therefore, its hemodynamics shows high flow and velocity in the malformed vascular mass. , chronic hypoperfusion of peripheral brain tissue and elevated venous pressure. There are two animal models of cerebral arteriovenous malformation, one is to form an arteriovenous fistula by anastomosis of extracranial and extracranial blood vessels in young animals, and the other is to use bioengineering technology to interfere with the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/04C12M1/34C12M1/04C12N5/071
CPCC12M21/08C12M25/12C12M29/00C12M29/04C12M29/10C12M29/18C12M41/40C12N5/069C12N2521/00
Inventor 赵明光张海峰
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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