Aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds

A two-phase system and sunflower seed technology, which is applied in the field of food and analytical chemistry, can solve the problems of complex method steps, cumbersome instrument operation, poor stability, etc., and achieve high extraction rate, short phase separation time, and low detection limit Effect

Inactive Publication Date: 2016-03-23
CHANGAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at defects such as complex method steps, cumbersome instrument operation, high cost and poor stability existing in the existing vitamin B6 detection method, the object of the present invention is to provide a two-phase aqueous system and its application for separating vitamin B6 in sunflower seeds , can improve the accuracy of residual vitamin B6 determination, shorten the detection cycle, and at the same time have the characteristics of easy operation, no use of large instruments, and less use of traditional organic volatile solvents to avoid secondary pollution

Method used

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  • Aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds
  • Aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds
  • Aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds

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preparation example Construction

[0035] 1. The preparation method of the sunflower seed solution to be separated: Grind a certain amount of sunflower seeds into powder, take 10g of sunflower seed ground powder and add double distilled water to stir, heat the solution to 60°C to increase the solubility of vitamin B6, quickly filter it in the dark, and put it into 100mL brown volumetric flask and constant volume, prepared as a solution of sunflower seeds to be separated for later use. Treat hazelnuts, almonds, walnuts, and peanuts in the same way to prepare hazelnuts to be separated, almonds to be separated, walnuts to be separated and peanuts to be separated.

[0036] 2. Take 3.5g of N-ethylpyridine nitrate, 1,3-dimethylimidazole iodide, N-ethylpyridine bromide, N-ethylpyridine iodide, and N-ethylpyridine tetrafluoroborate Put the ionic liquid in a centrifuge tube, add 2.5g of ammonium citrate, 4g of distilled water and 2g of sunflower seeds to be separated, and mix well. At 30°C and pH 5.0, shake in a consta...

Embodiment 1

[0060] Take 3.5g of N-ethylpyridine nitrate ionic liquid, put it in a centrifuge tube, add 2.5g of ammonium citrate, 4g of distilled water and 2g of the solution to be separated from sunflower seeds, and mix well. Constant temperature extraction at 30°C and pH 5.0 for 24 hours.

[0061] The concentration of remaining vitamin B6 in the water phase was measured, and the extraction rate of vitamin B6 was calculated as 99.0% from the content of vitamin B6 in the water phase before and after extraction. It shows that the invention has a good extraction effect on vitamin B6.

Embodiment 2

[0063] The preparation method in this example is the same as that in Example 1, the only difference being that the conditions in the preparation process are different. In this example, ammonium citrate is replaced by arylsulfonamide, and the rest of the conditions remain unchanged.

[0064] The concentration of remaining vitamin B6 in the water phase was measured, and the extraction rate of vitamin B6 was calculated from the content of vitamin B6 in the water phase before and after extraction to 74.2%. Show that this invention is general to the extraction effect of vitamin B6.

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Abstract

The invention discloses an aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds of same. The aqueous two-phase system is prepared from, by mass, 30-40% of N-ethyl pyridine nitrate ion liquid, 20-30% of ammonium citrate and the balance water, wherein the sum of the mass ratio of the N-ethyl pyridine nitrate ion liquid, the ammonium citrate and the water is 100%. The aqueous two-phase system has the advantages of being wide in linearity range, low in checkout limit, small in relative deviation and high in detection recovery rate of samples; the checkout requirement for residual vitamin B6 of the nation can be met, meanwhile, operation is simple, and the aqueous two-phase system is applicable to quantitative analysis of vitamin B6 in sunflower seeds.

Description

technical field [0001] The invention belongs to the technical field of food and analytical chemistry, and in particular relates to a two-phase aqueous system and its application for separating vitamin B6 from sunflower seeds. Background technique [0002] Vitamin B6 (VitaminB6), also known as pyridoxine, includes pyridoxine, pyridoxal and pyridoxamine, which have a close relationship and interaction with each other. It exists in the form of phosphate in the body. It is a water-soluble vitamin. It is easily destroyed by light or alkali, and it is not resistant to high temperature. It is an indispensable participant in various chemical reactions in the chemical factory of the human body. Vitamin B6 is a component of certain coenzymes in the human body, and participates in various metabolic reactions, especially closely related to amino acid metabolism. It mainly acts on the blood, muscles, nerves, skin, etc. of the human body. [0003] In the past ten years, the research on v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
Inventor 李宇亮卢雪谢娅婷卢筱佳孙钰琨
Owner CHANGAN UNIV
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