A two-phase aqueous system and its application in separating vitamin b6 from sunflower seeds

A two-phase aqueous system and sunflower seed technology, which is applied in the fields of food and analytical chemistry, can solve the problems of complicated method steps, cumbersome instrument operation, poor stability, etc., and achieve high extraction rate, short phase separation time, and low detection limit Effect

Inactive Publication Date: 2018-06-26
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at defects such as complex method steps, cumbersome instrument operation, high cost and poor stability existing in the existing vitamin B6 detection method, the object of the present invention is to provide a two-phase aqueous system and its application for separating vitamin B6 in sunflower seeds , can improve the accuracy of residual vitamin B6 determination, shorten the detection cycle, and at the same time have the characteristics of easy operation, no use of large instruments, and less use of traditional organic volatile solvents to avoid secondary pollution

Method used

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  • A two-phase aqueous system and its application in separating vitamin b6 from sunflower seeds
  • A two-phase aqueous system and its application in separating vitamin b6 from sunflower seeds
  • A two-phase aqueous system and its application in separating vitamin b6 from sunflower seeds

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preparation example Construction

[0035] 1. The preparation method of the solution of sunflower seeds to be separated: Grind a certain amount of sunflower seeds into powder, add 10g of sunflower seed powder to double distilled water and stir, heat the solution to 60℃ to increase the solubility of vitamin B6, quickly filter in the dark, and move in In a 100mL brown volumetric flask and constant volume, prepare the sunflower seed solution to be separated for later use. The samples of hazelnuts, almonds, walnuts and peanuts were processed in the same way to prepare hazelnuts to be separated, almonds to be separated, walnuts to be separated and peanuts to be separated.

[0036] 2. Take 3.5g of N-ethylpyridine nitrate, 1,3-dimethylimidazole iodide, N-ethylpyridine bromide, N-ethylpyridine iodide, and N-ethylpyridine tetrafluoroborate. In the centrifuge tube, add 2.5g of ammonium citrate, 4g of distilled water and 2g of sunflower seeds to the separated solution, and mix well. At 30°C and pH 5.0, shake in a constant te...

Embodiment 1

[0060] Take 3.5g of N-ethylpyridine nitrate ionic liquid, place it in a centrifuge tube, add 2.5g of ammonium citrate, 4g of distilled water and 2g of sunflower seed solution to be separated, and mix thoroughly. Extract at a constant temperature of 30°C and pH 5.0 for 24 hours.

[0061] The concentration of the remaining vitamin B6 in the water phase was determined, and the extraction rate of vitamin B6 was 99.0% calculated from the content of vitamin B6 in the water phase before and after extraction. It shows that this invention has a good extraction effect on vitamin B6.

Embodiment 2

[0063] The preparation method in this example is the same as that in Example 1, except that the conditions in the preparation process are different. In this example, ammonium citrate is replaced with arylsulfonamide, and other conditions remain unchanged.

[0064] The concentration of the remaining vitamin B6 in the water phase was measured, and the extraction rate of vitamin B6 was 74.2% calculated from the content of vitamin B6 in the water phase before and after extraction. It shows that the extraction effect of this invention on vitamin B6 is general.

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Abstract

The invention discloses an aqueous two-phase system and application of same to separation of vitamin B6 in sunflower seeds of same. The aqueous two-phase system is prepared from, by mass, 30-40% of N-ethyl pyridine nitrate ion liquid, 20-30% of ammonium citrate and the balance water, wherein the sum of the mass ratio of the N-ethyl pyridine nitrate ion liquid, the ammonium citrate and the water is 100%. The aqueous two-phase system has the advantages of being wide in linearity range, low in checkout limit, small in relative deviation and high in detection recovery rate of samples; the checkout requirement for residual vitamin B6 of the nation can be met, meanwhile, operation is simple, and the aqueous two-phase system is applicable to quantitative analysis of vitamin B6 in sunflower seeds.

Description

Technical field [0001] The invention belongs to the technical field of food and analytical chemistry, and specifically relates to a two-phase aqueous system and its application for separating vitamin B6 from sunflower seeds. Background technique [0002] Vitamin B6 (Vitamin B6) is also known as pyridoxine, which includes pyridoxine, pyridoxal and pyridoxamine, which have a close relationship and interaction with each other. It exists as a phosphate in the body. It is a water-soluble vitamin. It is easily destroyed by light or alkali, and is not resistant to high temperatures. It is an indispensable participant in various chemical reactions in the human body. Vitamin B6 is a component of certain coenzymes in the human body and participates in a variety of metabolic reactions, especially closely related to the metabolism of amino acids. It mainly acts on the blood, muscles, nerves, and skin of the human body. [0003] In the past ten years, the research of vitamin B6 analysis method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
Inventor 李宇亮杨鑫伍琪孟紫璇朱棋
Owner CHANGAN UNIV
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