Rapid protein staining solution
A dyeing solution and protein technology, which is applied in the field of rapid protein staining solution, can solve problems such as pungent odor and operator discomfort, and achieve the effect of saving time
Inactive Publication Date: 2016-03-30
QINGDAO AIDI BIOTECH CO LTD
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
First of all, both methanol and acetic acid have pungent smell, which can easily make the operator feel uncomfortable. Secondly, after the dyeing is completed, it needs to be decolorized with a decolorizing solution. The components of the decolorizing solution are methanol and acetic acid, which also have a pungent smell.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0009] Embodiment 1, this embodiment discloses a kind of fast protein staining liquid, comprises following composition: solute 20 grams, comprises Coomassie Brilliant Blue R-250 0.4-0.6%, sodium iodide 40-45%, sodium phosphate 15-20%, Sodium acetate 15-20% and metaphosphoric acid 20-25%; solvents include distilled water 95ml, absolute ethanol 5ml, concentrated HCl 350ul.
[0010] Put the film to be dyed into the staining solution, heat it in microwave for 10-15 seconds until the temperature of the liquid is 50-60°C, and dye it on the dyeing shaker for 30 minutes, then take out the film, rinse it with water for about 10 seconds, and the protein bands are ready presented.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a rapid protein staining solution characterized by comprising components as follows: 20 g of a solute comprising 0.4%-0.6% of Coomassie brilliant blue R-250, 40%-45% of sodium iodide, 15%-20% of sodium phosphate, 15%-20% of sodium acetate and 20%-25% of metaphosphoric acid, 95 ml of distilled water, 5 ml of absolute ethyl alcohol and 350 ul of concentrated HCl. The rapid protein staining solution only requires water and does not require methyl alcohol and acetic acid during destaining. Besides, it takes half an hour to stain, destaining is not required basically, protein stripes can present clearly, and the time is greatly saved.
Description
technical field [0001] The invention relates to a rapid protein staining solution. Background technique [0002] When laboratory experimenters perform protein staining, they generally use traditional staining solutions. The main components are Coomassie brilliant blue R-250, methanol and acetic acid. First of all, both methanol and acetic acid have pungent odors, which are easy to make the operator feel uncomfortable. Secondly, after the dyeing is completed, it is necessary to decolorize with a decolorizing solution. The components of the decolorizing solution are methanol and acetic acid, which also have a pungent odor. Finally, the staining step often takes more than 1 hour, and the decolorization also takes more than 2 hours. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a rapid protein staining solution. [0004] In order to solve the above technical problems, the present invention adopts the following t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 孔宁
Owner QINGDAO AIDI BIOTECH CO LTD