Aqueous bi-phase system and application thereof to extraction of vitamin B6 from watermelon pulp

An aqueous two-phase system and watermelon technology are applied in the application field of the two-phase system and the separation of vitamin B6 in watermelon pulp, which can solve the problems of long extraction time, low extraction rate, complicated operation, etc. High extraction rate and high purity

Inactive Publication Date: 2016-03-30
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the disadvantages of long extraction time, expensive equipment, complicated operation and low extraction rate in the existing vitamin B6 extraction metho

Method used

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  • Aqueous bi-phase system and application thereof to extraction of vitamin B6 from watermelon pulp
  • Aqueous bi-phase system and application thereof to extraction of vitamin B6 from watermelon pulp
  • Aqueous bi-phase system and application thereof to extraction of vitamin B6 from watermelon pulp

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] Example 1:

[0058] Take 5.51g of chlorinated-N-butylpyridinium ionic liquid, place it in a centrifuge tube, add 1.83g of KH 2 PO 4 And an appropriate amount of distilled water to make the total mass in the centrifuge tube 10g, then add 2g of watermelon pulp juice and mix well. Shake at a constant temperature at 30°C and pH 5.0 for 30 minutes, and then stand for 24 hours after phase separation.

[0059] After the phase separation is clear, the concentration of the remaining vitamin B6 in the water phase is determined, and the extraction rate of vitamin B6 is calculated as 98.9% from the content of vitamin B6 in the water phase before and after extraction. It shows that this invention has a good extraction effect on vitamin B6 in watermelon pulp juice.

Example Embodiment

[0060] Example 2:

[0061] The preparation method in this embodiment is the same as that in embodiment 1, except that the conditions in the preparation process are different. In this embodiment, the chlorinated-N-butylpyridine ionic liquid is changed to polyvinylpyrrolidone k30, and the other conditions remain unchanged.

[0062] After the phase separation is clear, the concentration of the remaining vitamin B6 in the water phase is determined, and the extraction rate of vitamin B6 is calculated to be 73.6% from the content of vitamin B6 in the water phase before and after extraction. It shows that the extraction effect of this invention on vitamin B6 in the watermelon pulp juice is general.

Example Embodiment

[0063] Example 3:

[0064] The preparation method in this example is the same as that in Example 1, except that the conditions in the preparation process are different. In this example, potassium dihydrogen phosphate is changed to an equivalent amount of sodium sulfite, and the other conditions remain unchanged.

[0065] After the phase separation is clear, the concentration of the remaining vitamin B6 in the water phase is determined, and the extraction rate of vitamin B6 is calculated from the content of vitamin B6 in the water phase before and after extraction to 82.6%. It shows that the extraction effect of this invention on vitamin B6 in the watermelon pulp juice is general.

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Abstract

The invention discloses an aqueous bi-phase system and application thereof to extraction of vitamin B6 from watermelon pulp. The aqueous bi-phase system comprises the following raw materials by mass: 50 to 70.1 percent of n-butylpyridium chloride ionic liquid, 15.3 to 24.2 percent of monopotassium phosphate and the balance of water, wherein the total percentage of the three ingredients is 100 percent. The extraction rate of vitamin B6 from watermelon pulp can reach up to 98.9 percent according to the application. The application has the advantages that the linearity range is wide; the detection limit is low; the relative standard deviation is relatively low; the detection recovery rate to a sample is high; the extraction effect is favorable; a large quantity of vitamin B6 can be provided for various fields such as biological medicine due to the aqueous bi-phase system and the application.

Description

technical field [0001] The invention belongs to the environmental protection technical field of environmental engineering and analytical chemistry, and specifically relates to a two-phase aqueous system and its application for separating vitamin B6 from watermelon pulp. Background technique [0002] Vitamin B6 (VitaminB6), also known as pyridoxine, is a water-soluble vitamin that is easily destroyed by light or alkali and is not resistant to high temperatures. It is a component of some coenzymes in the human body and participates in various metabolic reactions of the human body, especially closely related to amino acid metabolism. Vitamin B6 slows down insulin therapy for diabetic vascular complications, which are caused by arteriosclerosis. The activated form of vitamin B6, pyridoxalphosphate (PDP), has the function of protecting vascular endothelial cells and reducing the damage of endothelial cells from activated platelets, and inhibits platelet aggregation and blood coa...

Claims

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Application Information

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IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 李宇亮徐一丹董婷婷卢筱佳孙钰琨
Owner CHANGAN UNIV
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