A method for detecting miRNAs based on a superhydrophilic microwell sensing interface
A sensing interface and super-hydrophilic technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low accuracy of measurement results, prone to cross-reaction, high professional requirements, etc., to achieve the purpose of testing samples Small amount, stable test results, and simple preparation
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Embodiment 1
[0020] Take ordinary glass slides, soak them in washing solution (mixture of 98% sulfuric acid and 30% hydrogen peroxide solution, 3:1), and heat at 80°C for 1h. After the slides are cooled, take them out and place them in acetone, ethanol, and deionized water in turn, ultrasonically clean them for 10 minutes at room temperature, and finally blow them off with nitrogen, and put them in a clean Petri dish for later use. Light the usual white paraffin candle, place the cleaned glass slide above the candle flame and move it horizontally at a constant speed for 6 seconds, and the black soot particles can evenly adhere to the surface of the slide. Take 2mL tetraethyl orthosilicate and 2mL ammonia water respectively and place them in airtight containers, put them into glass slides with soot attached to them for vapor phase deposition, react at room temperature for 24 hours and take them out to get soot substrates wrapped in silica. The sample was put into a muffle furnace for calcin...
Embodiment 2
[0025] Take the quartz substrate, soak it in the washing solution (98% sulfuric acid and 30% hydrogen peroxide solution, 3:1 mixture), and heat at 80°C for 1h. After the substrate is cooled, take it out and put it into acetone, ethanol and deionized water in turn, ultrasonically clean it at room temperature for 10 minutes, and finally blow it off with nitrogen, and put it in a clean petri dish for later use. Light the usual white paraffin candle, place the cleaned substrate above the candle flame and move it horizontally at a constant speed for 6 seconds, and the black soot particles can evenly adhere to the surface of the substrate. Take 2mL tetraethyl orthosilicate and 2mL ammonia water respectively and put them in airtight containers, put the substrate with soot attached at the same time for vapor phase deposition, react at room temperature for 24h and take it out to get the soot substrate wrapped with silica. The sample was calcined at 600°C for 2 hours in a muffle furnace...
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