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Enteromorpha polysaccharide biological activity improving method

A technology of Enteromorpha polysaccharides and biological activity, which is applied in the direction of drug combination, anti-toxic agents, antibacterial drugs, etc., can solve the problem of not simultaneously improving the antibacterial and antioxidant activities of Enteromorpha polysaccharides, and achieve the effect of improving the antioxidant activity in vitro

Active Publication Date: 2016-05-04
ZHEJIANG GONGSHANG UNIVERSITY
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  • Claims
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Problems solved by technology

It has been reported in the literature that the sulfation, phosphorylation and acetylation of Enteromorpha polysaccharides have been modified, and the antioxidant activity of the modified Enteromorpha polysaccharides has been significantly improved; it has also been reported that the antibacterial activity of Enteromorpha polysaccharides has been improved after selenization, but there is no simultaneous Report on the method of improving the antibacterial and antioxidant activities of Enteromorpha polysaccharides

Method used

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  • Enteromorpha polysaccharide biological activity improving method
  • Enteromorpha polysaccharide biological activity improving method
  • Enteromorpha polysaccharide biological activity improving method

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Embodiment 1-1

[0037] Embodiment 1-1, the preparation of hydroxamation degradation enteromorpha polysaccharide:

[0038] (1) Weigh 1g of Enteromorpha crude polysaccharide (EP) and dissolve it in 100mL of distilled water, heat in a water bath at 30°C, add vitamin C and hydrogen peroxide in sequence, so that the final concentration of both is 9mmol / L, keep stirring and react for 2h, Concentrate the reaction solution (concentrate under reduced pressure at 60-70°C) to 1 / 3 of the original volume, precipitate with 4 times the volume of the concentrated solution with 95% ethanol, overnight at 4°C, centrifuge (8000rpm, 20min), take the precipitate and reconstitute it with water (the ratio of the amount of the precipitate to water is about 20 mg / ml), dialyzed with a dialysis bag with a molecular weight cut-off of 3500 Da for 48 hours, and freeze-dried the dialysate (freeze-dried at -50°C for 24 hours) to obtain powdery degraded Moss polysaccharide (DEP).

[0039] (2) Dissolve 0.3 g of degraded Enter...

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Abstract

The present invention discloses an enteromorpha polysaccharide biological activity improving method comprising the following steps: enteromorpha polysaccharide is degraded by hydrogen peroxide-vitamin C combined method to obtain degraded enteromorpha polysaccharide (DEP); chloroacetic acid as a carboxymethylation reagent is reacted with the degraded enteromorpha polysaccharide (DEP) to prepare carboxymethylated enteromorpha polysaccharide (CDEP); and in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), the CDEP is coupled with hydroxylamine to obtain hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP). The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has a relatively low molecular weight (30-13kDa), and iron-chelating capacity is 0.2-1.2mmol / g. The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has high in-vitro antioxidant activity and antibacterial activity.

Description

technical field [0001] The invention belongs to the technical field of chemistry and chemical engineering, and relates to a method for enhancing the bioactivity of the degraded enteromorpha polysaccharide through hydroxamation modification. Background technique [0002] Enteromorpha is a large-scale economical green algae in the southeast coast of my country. It is rich in resources and can be eaten by itself. It also contains a variety of active substances, such as Enteromorpha polysaccharides, lipid pigments, and phenols. According to literature reports, Enteromorpha polysaccharide has physiological functions such as improving immunity of mammals, lowering blood fat, anti-inflammatory, antibacterial and anti-oxidation. Therefore, the development of Enteromorpha into functional food has a good prospect. The polysaccharides extracted from Enteromorpha enteromorpha are mainly water-soluble sulfated polysaccharides with large molecular weight and relatively low biological act...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P31/04
CPCC08B37/00
Inventor 周涛许杰
Owner ZHEJIANG GONGSHANG UNIVERSITY
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