Human calcitonin-cucurbituril compound preparation and preparation method thereof

A technology of human calcitonin and compound preparations, which is applied in the direction of pharmaceutical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., which can solve the problem of decreased biological activity, affecting the therapeutic effect, and easily causing thyroid tumors, etc. problem, to achieve the effect of inhibiting fibrosis and improving biological activity

Active Publication Date: 2016-05-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the amino acid sequence of calcitonin from non-human sources is quite different from that of human calcitonin, for example, the homology between sCT and hCT is only 50%. Chlorin is an immune reactant, and the human body's immune response to it will affect its clinical application value
Although sCT is often used in the clinical treatment of osteoporosis and hypercalcemia, due to its poor homology with hCT, the immune response and antibodies generated during application will affect the therapeutic effect
[0004] Although hCT is used to treat osteoporosis and hyp

Method used

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  • Human calcitonin-cucurbituril compound preparation and preparation method thereof
  • Human calcitonin-cucurbituril compound preparation and preparation method thereof
  • Human calcitonin-cucurbituril compound preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] Add 2.0 mg of human calcitonin (hCT) and 6.9 mg of cucurbituril 7 (CB[7]) into the 1# control bottle that is equipped with a stirrer, add hCT2.0mg, CB[7]17.4mg to the already filled Add hCT2.0mg and CB[7]34.8mg to the 3# control bottle with the stirrer in the 2# control bottle of the stirring bar. The molar ratio of hCT to CB[7] in the 1~3# control bottle They are 1:10, 1:25, and 1:50.

[0029] Add 3 mL of PBS buffer with a pH of 7.4 to 1~3# control bottles, and place them in an ice bath until the hCT and CB[7] are completely dissolved and mixed, and then the hCT-CB[7] compound preparation is obtained. Then put the 1~3# control bottle in a water bath at 40℃, and use a thermostatic magnetic stirrer to stir at a speed of 200r / min. From the 0th to 4th hour of the start of stirring, take samples every 0.5h. Samples were taken at intervals of 1 hour from the 4th to 20th hours, and the stirring was stopped after 20 hours of stirring.

[0030] After each sample ...

Example Embodiment

[0037] Example 2

[0038] Measure hCT and CB[7] according to the molar ratio of hCT to CB[7]=1:10, add hCT and CB[7] to the control bottle equipped with a stir bar, and add deionized water to the control bottle at room temperature , Shake the control bottle to completely dissolve hCT and CB[7]. The amount of deionized water should be such that the concentration of hCT is 100μmol / L and the concentration of CB[7] is 1mmol / L. Then use a magnetic stirrer at 25℃ to 500r Stir for 20 minutes at a speed of 1 / min, freeze-dry, and obtain the hCT-CB [7] composite formulation.

Example Embodiment

[0039] Example 3

[0040] Measure hCT and CB[7] according to the molar ratio of hCT to CB[7]=1:30, add hCT and CB[7] to the control bottle equipped with a stir bar, and add the pH value to the control bottle at room temperature 7.4 PBS buffer solution, shake the control bottle to completely dissolve hCT and CB[7], the amount of PBS buffer should be such that the concentration of hCT is 100μmol / L and the concentration of CB[7] is 3mmol / L, and then the control The bottle was placed in a water bath at 40° C., and the control bottle was stirred for 30 minutes with a constant temperature magnetic stirrer at a speed of 100 r / min, and then freeze-dried to obtain the hCT-CB [7] composite formulation.

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Abstract

The invention provides a human calcitonin-cucurbituril compound preparation. The compound preparation is prepared from human calcitonin-cucurbituril supermolecular complex, human calcitonin and cucurbituril. A preparation method of the human calcitonin-cucurbituril compound preparation comprises the steps that human calcitonin and cucurbituril are dissolved in a water-borne solvent with the pH value of 7-8 at the temperature of 0 to 40 DEG C and evenly mixed, and the human calcitonin-cucurbituril compound preparation is obtained; or human calcitonin and cucurbituril are dissolved in a water-borne solvent with the pH value of 7-8 at the temperature of 0 to 40 DEG C and evenly mixed, then freeze drying is carried out, and the human calcitonin-cucurbituril compound preparation is obtained. The human calcitonin-cucurbituril compound preparation is not prone to fibrosis in the use process, the biological activity of the compound preparation is higher than that of independent human calcitonin, and the human calcitonin is beneficial for promoting clinical application of human calcitonin.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a human calcitonin-cucurbitacin compound preparation and a preparation method thereof. Background technique [0002] Calcitonin (CT) is a linear polypeptide hormone containing 32 amino acids, which is secreted by the parafollicular cells (parafollicular cells, also known as C cells) of the hindbranch body of vertebrates and the thyroid gland of mammals. , has its corresponding receptors in bone and kidney, has the function of regulating calcium and phosphorus metabolism, and is currently mainly used for the treatment of diseases related to low bone mass. The secretion of calcitonin is related to the calcium concentration in the blood flowing through the thyroid gland, so the increase of blood calcium concentration can cause the increase of calcitonin secretion and inhibit bone resorption, and reduce the blood calcium concentration of patients with hypercalcemia. [0003] The amino aci...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K38/23A61K47/69
CPCA61K38/23
Inventor 李建树商惠丁春梅刘衍朋陈星羽
Owner SICHUAN UNIV
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