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Application of SNP Molecular Marker of Rice Grain Cadmium Content Related Gene lcd-22

A rice grain, tagsnp-266272 technology, applied in the application field of SNP molecular markers, can solve the problems of yield impact, food unsuitable for consumption, long and difficult treatment period of cadmium-contaminated farmland, etc.

Active Publication Date: 2019-03-08
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In cadmium-contaminated cultivated land, 5-10% of the area yield is seriously affected, and the food produced is not suitable for consumption
The safety of food crops is closely related to human health, but the treatment cycle of cadmium-contaminated farmland is long and difficult. Therefore, how to produce safe food crops without wasting existing farmland is particularly important

Method used

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  • Application of SNP Molecular Marker of Rice Grain Cadmium Content Related Gene lcd-22
  • Application of SNP Molecular Marker of Rice Grain Cadmium Content Related Gene lcd-22
  • Application of SNP Molecular Marker of Rice Grain Cadmium Content Related Gene lcd-22

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Embodiment 1

[0034] Example 1, TagSNP-266272 is a SNP molecular marker associated with rice grain cadmium content

[0035] 1. Discovery of TagSNP-266272

[0036]According to the cadmium content in the grains of 65 domestic and foreign rice varieties and the results of genome sequencing, the inventors of the present invention screened out a SNP site related to the cadmium content in rice grains. The SNP site corresponds to chromosome 6 of Nipponbare Rice. 266272, the SNP site was named TagSNP-266272. TagSNP-266272 is a biallelic polymorphic SNP site of the 69th nucleotide of SEQ ID No.1 on rice chromosome 6, and the 69th nucleotide of SEQ ID No.1 is C or T . The 69th position of SEQ ID No.1 (TagSNP-266272) is the homozygous TagSNP-266272 genotype named CC, and the 69th position of SEQ ID No.1 (TagSNP-266272) is the homozygous type of T The TagSNP-266272 genotype of the tagSNP-266272 was named TT, and the 69th position of SEQ ID No.1 (TagSNP-266272) was a heterozygous TagSNP-266272 genoty...

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Abstract

The invention discloses the application of an SNP (Single Nucleotide Polymorphism) molecular marker of a gene LCd-22 related to the Cd content in rice grains. The application specifically can be the application of TagSNP-266272 in identifying or auxiliarily identifying the Cd content in the rice grains, wherein the TagSNP-266272 is an SNP site of a rice genome, is a nucleotide in a sixty-ninth position of an SEQ ID No.1 on a sixth chromosome of rice and is C or T. An experiment shows that the Cd content in grains of 94 percent of gene type TT rice varieties is lower than 0.2 mg / kg, and the Cd content in the grains of 94 percent of gene type CC rice varieties is higher than 0.2 mg / kg, so that the TagSNP-266272 is verified to be the SNP molecular marker related to the Cd content in the rice grains.

Description

technical field [0001] The invention relates to the application of SNP molecular markers of rice grain cadmium content-related genes in the field of biotechnology, in particular to the application of SNP molecular markers of rice grain cadmium content-related genes LCd-22. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) refers to the variation of a single nucleotide in the genome, which is the smallest variation unit, and is a variation formed by a single nucleotide pair substitution, transversion, insertion or deletion form. [0003] Rice is one of the most important food crops in the world, and more than half of the population uses rice as a staple food. Heavy metal pollution in farmland is a major environmental problem facing the world today and has received widespread attention from all walks of life. Heavy metal pollution is complex, concealed, and persistent, and may endanger human health through the enrichment of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/156
Inventor 何振艳麻密许文秀闫慧莉
Owner INST OF BOTANY CHINESE ACAD OF SCI