GeXP multi-primers and method for detecting akabane viruses, bovine viral diarrhea viruses and foot and mouth disease viruses

Abstract

The invention discloses GeXP multi-primers and a method for detecting akabane viruses, bovine viral diarrhea viruses and foot and mouth disease viruses. The GeXP multi-primers and the method have the advantages that the method is created for detecting diversified epidemic diseases of cows on the basis of GeXP multi-gene expression genetic analysis systems, so that problems that serological methods are low in sensitivity, only single pathogens can be detected by the aid of conventional PCR (polymerase chain reaction), gene chip methods are high in cost and the like when diversified epidemic diseases of cows are about to be detected at present can be solved, and reference of RNA (ribonucleic acid) positive samples of three epidemic diseases of the cows is created; 10-times continuous dilution reference of reference RNA is used as a detection object, and the detection sensitivity can reach 10-100 copies; cross reaction between the GeXP multi-primers and the other epidemic diseases of the cows and false-negative results can be prevented; monitoring and screening requirements on large quantities of epidemic diseases of cows can be met by 600 actual detection samples.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to the simultaneous detection and identification of three imported cow virus diseases. Background technique [0002] Foot and Mouth Disease (FMD), Bovine Viral Diarrhea (BVD) and Akabane Disease (AKA) are three viral diseases that require isolation and quarantine for imported dairy cows. In recent years, as the national demand for high-quality dairy products has increased, the number of imported dairy cows has increased year by year, which has brought a certain amount of work pressure and financial burden to animal disease testing laboratories at dairy cow entry ports. Therefore, it is of great significance to establish a high-throughput rapid detection method that can simultaneously differentially diagnose multiple dairy cow diseases to reduce the workload of the inspection laboratory at the port of entry of dairy cows, reduce the cost of inspection, and carry out epidemiol...

AI Technical Summary

Problems solved by technology

Virus isolation and culture is the gold standard for diagnosis, but there are problems such as long test period and cumbersome operation; virus neutralization test requires specific standard serum or virus strain, which has certain limitations in practical application; imported enzyme-linked immunosorbent immunoassay Kits are generally expensive; molecular biology methods such as ordinary PCR and fluorescent quantitative PCR are all aimed at the detection of a single virus, which cannot meet the detection needs of differential diagnosis of mixed infections with multiple pathogens

Multiplex PCR technology has been applied to the differential diagnosis of various pathogens, but due to the problem of amplification preference, it is impossible to realize high-throughput detection in the true sense

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