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A Strain of Pseudomonas Korea and Its Application

A technology of Pseudomonas and strains, applied in the field of microorganisms and biological control, can solve the problems of relocation of production areas, authenticity distortion, production area and scale reduction, etc., and achieve strong cellulose degradation ability and good effect

Inactive Publication Date: 2019-03-12
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation and planting process of Pseudostellariae Pseudostellaria, there are serious continuous cropping obstacles. Continuous cropping Pseudostellariae Pseudostellariae plants do not grow well, and the underground part cannot expand normally (such as figure 1 As shown), after harvesting, it must be replanted after 2~4 years
The problem of continuous cropping obstacles has also led to a series of downstream unfavorable problems. For example, the production area and scale of Taizisandao are shrinking year by year, and even the production area has moved out, and authenticity is distorted.

Method used

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  • A Strain of Pseudomonas Korea and Its Application
  • A Strain of Pseudomonas Korea and Its Application
  • A Strain of Pseudomonas Korea and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 antagonistic bacteria Pseudomonas korean ( Pseudomonas koreansis ) Screening and identification of CJ-361

[0022] 1. The antagonistic bacteria Pseudomonas korean ( Pseudomonas koreansis ) Screening of CJ-361

[0023] Antagonistic bacteria Pseudomonas korean ( Pseudomonas koreansis ) CJ-361 was screened from the rhizosphere soil of Pseudostellaria heterophylla.

[0024] 1.1 Preparation of Pseudomonas culture medium

[0025] Pseudomonas selective isolation agar (PSIA) preparation method is as follows: Weigh 20 g of soybean-casein digest agar (soybean casein digest agar, SCD) (BD, USA), add 495.5 mL Heat and stir in double-distilled water, after fully dissolving, add 1 mL of 0.1% (wt / vol) crystal violet stock solution, sterilize at 121°C for 15 minutes at high temperature and high pressure, cool to about 50°C, add to the medium 3.5 mL of 5% (wt / vol) furanidine stock solution was mixed thoroughly, poured quickly, cooled and solidified to prepare a Pseu...

Embodiment 2

[0037] Example 2 Bacteriostatic effect detection of antagonistic bacteria

[0038] Using PDA medium, inoculate activated Pseudomonas korean CJ-361 at a distance of 2.5 cm from the center of the circle, place it in a constant temperature incubator at 28°C for 48 hours, and inoculate it in the center of the dish with pathogenic fungi ( For example, Fusarium oxysporum and Fusarium moniliforme of Pseudostellariae heterophylla) were placed in a constant temperature incubator at 28°C in the dark for several days, and the formation of the inhibition zone was observed in real time. After culturing for 5 days, it was found that Pseudomonas Korea ( Pseudomonas koreansis CJ-361) can effectively antagonize the hyphal growth of Pseudostellariae-specific Fusarium oxysporum and Fusarium moniliforme (such as image 3 shown).

Embodiment 3

[0039] Embodiment 3 Pseudomonas Korea ( Pseudomonas koreansis CJ-361) optimal medium optimization

[0040] Prepare the following medium against Pseudomonas korean ( Pseudomonas koreansis CJ-361) optimized growth medium, the medium is as follows: 1) Full LB solution; 2) 1 / 4 LB solution; 3) 1 / 4LB+1 / 20MS; 4) 1 / 4LB+1 / 40MS ;5) 1 / 4LB+1 / 80MS; 6) 1 / 4LB+1 / 20MS +0.1wt.% brown sugar; 7) 1 / 4LB+1 / 40MS+0.1wt.% brown sugar; 8) 1 / 4LB+1 / 80MS+0.1wt.% brown sugar.

[0041] Among them, the formula of MS medium (both without iron salt) is: 1) macroelements: 1900 mg / L KNO 3 , 1650 mg / LNH 4 NO 3 , 370 mg / L MgSO 4 •7H 2 O, 170 mg / L KH 2 PO 4 , 440 mg / L CaCl 2 • 2H 2 O; 2) Trace elements: 22.3mg / L MnSO 4 • 4H 2 O, 8.6 mg / L ZnSO 4 •7H 2 O, 6.2 mg / L H 3 BO 3 , 0.83 mg / L KI, 0.25 mg / LNa 2 MoO 4 •7H 2 O, 0.025 mg / L CuSO 4 • 5H 2 O, 0.025 mg / L CoCl 2 •6H 2 O; 3) Organic matter: 2.0 mg / L glycine, 0.5 mg / L pyridoxine hydrochloride, 0.1 mg / L ammonium sulfate hydrochloride, 0.5 mg / L ...

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PUM

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Abstract

The invention provides a strain of Pseudomonas Koreana and its application, which has been identified as Pseudomonas Koreana CJ-361, and the preservation number is CGMCC NO.12122. The Pseudomonas CJ‑361 disclosed in the invention is obtained by using the Pseudomonas selective medium to isolate from the rhizosphere soil of Pseudomonas pseudomonas, and the Fusarium oxysporum isolated from the root lesions of Pseudomonas heterophylla by means of plate confrontation culture method , Fusarium moniliforme for antagonistic experiments, found that the bacteria have a strong antagonistic effect on these two pathogenic bacteria. Simultaneously, the present invention provides the optimum culture medium for the growth of the antagonistic bacteria. The optimum formula is 1 / 4 LB+1 / 20 MS (without iron salt)+0.1wt.% brown sugar, and the fermented bacterial liquid has obvious antagonistic effect. The antagonistic strain and the microbial agent thereof provided by the invention can effectively prevent and cure the root rot disease of heterophylla, and provide an environmentally friendly, ecological and safe biological control potential strain for overcoming or alleviating the replanting disease of heterophylla.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a Korean pseudomonas strain and application thereof, which can be used to overcome or alleviate the problem of soil-borne diseases of continuous cropping heterophylla, and belongs to the technical field of microorganisms and biological control. Background technique [0002] Pseudostellaria ( Pseudostellaria heterophylla ) alias children's ginseng, baby ginseng, rice ginseng, is a perennial herbaceous plant of Caryophyllaceae, and its roots are used as medicine. Included in the "List of Chinese herbal medicines that can be used in health food". Pseudostellaria is mainly produced in Fujian, Guizhou, Anhui and other places, among which Zherong County in Fujian Province has the longest cultivation history and the best quality, and is known as the "Hometown of Chinese Pseudostellaria". During the cultivation and planting process of Pseudostellariae Pseudostellaria, there are serious continuous ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 陈军吴林坤林文雄林翰黄珊瑜吴艳红林生
Owner FUJIAN AGRI & FORESTRY UNIV