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Method for preparing polymalic acid by adding oxygen carriers

A technology of polymalic acid and system, which is applied in the field of preparing polymalic acid by adding an oxygen carrier, to achieve the effects of increasing conversion rate, improving synthesis efficiency and reducing production cost

Inactive Publication Date: 2016-05-18
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the invention patent with application number 201410830995.4 uses fed-batch fermentation to increase the yield of polymalic acid by 11-20%, but so far there is no technical solution to promote synthesis by adding oxygen carriers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Seed cultivation

[0043] Activate the Aureobasidium pullulans strain, wash the spores with sterile physiological saline to obtain a spore suspension; transfer the 10% inoculum to a 500ml baffle bottle containing 100ml seed medium, culture at 25°C, shake The bed speed is 200r / min, and the incubation time is 40h;

[0044] The composition of the seed medium is: sucrose 140g / L, yeast extract 3g / L, ammonium sulfate 1g / L, succinic acid 2g / L, corn steep liquor 0.1%v / v, K 2 CO 3 0.4g / L, MgSO 4 ·7H 2 O0.1g / L, KH 2 PO 4 1g / L, ZnSO 4 ·7H 2 O0.05g / L, the rest is distilled water;

[0045] (2) Fermentation culture

[0046] The inoculation port was under flame protection, and the obtained seed liquid was connected to a 5L fermenter with 3L fermentation liquid in an amount of 10% (v / v) for fermentation and cultivation; the cultivation conditions were rotation speed 300r / min, ventilation ratio 1:2 , the tank pressure is 0.1Mpa, when the fermentation is carried out to 40h,...

Embodiment 2

[0051] (1) Seed cultivation

[0052] The Aureobasidium pullulans strain was activated for 2.5 hours, and the spores were washed with sterile physiological saline to obtain a spore suspension. According to the 10% inoculum amount, it was received in a 500ml baffle bottle containing 100ml seed medium, the cultivation temperature was 20°C, the shaker speed was 180r / min, and the cultivation time was 35h;

[0053] The composition of the seed medium is: sucrose 140g / L, yeast extract 3g / L, ammonium sulfate 1g / L, succinic acid 2g / L, corn steep liquor 0.1%v / v, K 2 CO 3 0.4g / L, MgSO 4 ·7H 2 O0.1g / L, KH 2 PO 4 1g / L, ZnSO 4 ·7H 2 O0.05g / L, the rest is distilled water;

[0054] (2) Fermentation culture

[0055] The inoculation port was under flame protection, and the resulting seed solution was placed in a 5L fermenter with 3L fermentation broth in an amount of 8% (v / v) for fermentation. The culture conditions are 280r / min rotation speed, 1:2 ventilation ratio, 0.2Mpa tank pressu...

Embodiment 3

[0060] (1) Seed cultivation

[0061] Activate the Aureobasidium pullulans strain for 3 hours, wash the spores with sterile physiological saline to obtain a spore suspension; transfer the 10% inoculum into a 500ml baffle bottle containing 100ml seed medium, and cultivate at a temperature of 30°C. The rotating speed of the shaker is 230r / min, and the incubation time is 45h;

[0062] The composition of the seed medium is: sucrose 140g / L, yeast extract 3g / L, ammonium sulfate 1g / L, succinic acid 2g / L, corn steep liquor 0.1%v / v, K 2 CO 3 0.4g / L, MgSO 4 ·7H 2 O0.1g / L, KH 2 PO 4 1g / L, ZnSO 4 ·7H 2 O0.05g / L, the rest is distilled water;

[0063] (2) Fermentation culture

[0064] The inoculation port was under the protection of the flame, and the obtained seed liquid was connected to a 5L fermenter with 3L fermentation liquid in an amount of 12% (v / v) for fermentation and cultivation; the cultivation conditions were a rotation speed of 320r / min, and a ventilation ratio of 1:2 ...

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Abstract

The invention discloses a method for preparing polymalic acid by adding oxygen carriers. Budding short-stalk mucedine strains serve as original strains, and spore suspension liquid is prepared; the spore suspension liquid is transferred to a seed culture medium to be cultured, the culture temperature ranges from 20 DEG C to 30 DEG C, the rotating speed of a shaking table ranges from 180 r / min to 230 r / min, and the culture time ranges from 35 h to 45 h; under the protection of flame, the obtained seed liquid is transferred to a fermentation medium at the amount of 8-12 %v / v to be cultured in a fermentation mode; a 10 mol / L H2O2 solution is added in a flowing mode when fermentation is conducted to the logarithmic phase of 35-50 h, wherein the volume of the H2O2 solution is 0.5-2 % of the volume of fermentation liquor, the fermentation period ranges from 140 h to 150 h, the H2O2 solution added in the logarithmic phase in the flowing mode promotes the oxygen dissolving rate, and therefore the synthetic efficiency of the polymalic acid is improved, the conversion rate of substrates is increased, the production cost is reduced, and efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing polymalic acid by adding an oxygen carrier. Background technique [0002] Polymers play a pivotal role in today's world and even in the future, especially in the development of new alternative energy sources, and play an irreplaceable role in saving resources, energy and protecting the ecological environment. Polymers in the new era are inseparable from all walks of life. Scientists will explore polymers in more aspects, echoing the low-carbon and energy-saving life of the era, and polymers and products will become more and more adaptable theme of this era. Facts show that high molecular polymers have achieved rapid development in recent years, and many important high molecular polymers have been industrialized one after another. [0003] Polymalic acid is a homopolymeric polymer synthesized from malic acid as the only monomer. It has uniqu...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/645
CPCC12P7/62
Inventor 乔长晟边艳慧李雪李洪
Owner 天津北洋百川生物技术有限公司
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