Preparation method of gastroenteritis virus monoclonal antibody biological immune preparation
A technology of monoclonal antibodies and biological preparations, applied in antiviral agents, antibodies, pharmaceutical formulations, etc., can solve the problems of serious environmental pollution, complicated production process, high cost of finished products, etc., to overcome more processes, ensure product quality, improve The effect of production efficiency
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[0011] The preparation method of the three gastroenteritis virus monoclonal antibody immune biological preparations of the present embodiment comprises the following steps:
[0012] (1) Inject 0.7ml of high-pressure sterilized paraffin oil into the abdominal cavity of the mouse, inject 107 hybridoma cells into the abdominal cavity of the mouse on the 6th day, and extract the ascites when the mouse’s abdomen is extremely distended, and centrifuge the ascites at 3500rpm 12min, obtain supernatant porcine transmissible gastroenteritis virus monoclonal antibody;
[0013] (2) Grind Radix isatidis, take 1 part of its powder, add 12 parts of distilled water, soak for 15 hours, place in a water bath at 70 ° C for 12 minutes, filter, and concentrate the filtrate under reduced pressure at 70 ° C and a negative pressure of 8 kPa , adding ethanol to the concentrated solution to make the alcohol content reach 70%, and the supernatant is the extract;
[0014] (3) Porcine transmissible gastr...
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