Woad triploid breeding method with obvious rhizome weight increase

A technology of isatis and rhizomes, applied in the field of isatis triploidy breeding, can solve the problems of low survival rate, low mutation rate of breeding methods, huge consumption of manpower and material resources, etc., and achieves high nutritional value, rich yield and good applicability Effect

Inactive Publication Date: 2016-06-15
周常
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the breeding method of this patent has low mutation rate and low survival rate, and requires a large number of seeds for ion beam mutagenesis, which consumes a lot of manpower and material resources.

Method used

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  • Woad triploid breeding method with obvious rhizome weight increase

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain

[0032] Include the following steps:

[0033] Step 1, seed germination: select Isatis indica seeds, wash them three times with sodium hypochlorite: chlorine dioxide as 5:1 sterile water, then soak them in hot water at 35°C for 1 hour, remove them, and transplant them into the liquid nutrient medium. Under the condition that the temperature is 25°C and the rotation speed of the medium is 15r / min, dark culture is carried out. After germination, the temperature is 28°C and the light intensity is 2500lx. bud. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 300mg / L, K 2 SO 4 , 100mg / L, KNO 3 , 120mg / L, (NH 4 ) 2 HPO 4 , 150mg / L, CaCl 2 , 50mg / L, ZnSO 4 , 50mg / L, NaCl, 15mg / L, MgCl 2 , 80mg / L, FeCl 2 , 20mg / L, Mg(BO 2 ) 2 , 180mg / L, vitamin A, 20mg / L, folic acid, 10mg / L, niacinamide, 50mg / L, vitamin E, 20mg / L, phenylpropanamide, 100mg / L, cysteine...

Embodiment 2

[0040] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain

[0041] Include the following steps:

[0042] Step 1, seed germination: select Isatis indica seeds, wash them with sodium hypochlorite: chlorine dioxide 1:1 disinfectant water for 5 times, then soak them in hot water at 50°C for 2 hours, remove them, and transplant them into the liquid nutrient medium. Under the condition that the temperature is 30°C and the rotation speed of the medium is 30r / min, dark culture is carried out. After germination, the temperature is 33°C and the light intensity is 3200lx. bud. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 500mg / L, K 2 SO 4 , 150mg / L, KNO 3 , 180mg / L, (NH 4 ) 2 HPO 4 , 250mg / L, CaCl 2 , 80mg / L, ZnSO 4 , 100mg / L, NaCl, 50mg / L, MgCl 2 , 100mg / L, FeCl 2 , 50mg / L, Mg(BO 2 ) 2 , 300mg / L, vitamin A, 50mg / L, folic acid, 30mg / L, niacinamide, 150mg / L, vitamin E, 60mg / L, phenylpropanamide, 180mg / L, cy...

Embodiment 3

[0049] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain

[0050] Include the following steps:

[0051] Step I, seed germination: select Isatis indica seeds, wash them with sodium hypochlorite: chlorine dioxide 3:1 disinfectant water for 4 times, then soak them in hot water at 43°C for 1.5 hours, remove them, and transplant them into liquid nutrient medium , under the conditions of a temperature of 28°C and a rotation speed of the medium of 23r / min, dark culture was carried out. After germination, the temperature was 31°C and the light intensity was 2850lx. Woad sprouts. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 350mg / L, K 2 SO 4 , 130mg / L, KNO 3 , 150mg / L, (NH 4 ) 2 HPO 4 , 230mg / L, CaCl 2 , 60mg / L, ZnSO 4 , 70mg / L, NaCl, 30mg / L, MgCl 2 , 90mg / L, FeCl 2 , 30mg / L, Mg(BO 2 ) 2 , 270mg / L, vitamin A, 30mg / L, folic acid, 25mg / L, niacinamide, 120mg / L, vitamin E, 35mg / L, phenylpropanamide, 140mg / L...

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Abstract

The invention discloses a woad triploid breeding method with obvious rhizome weight increase.The method comprises the following steps of germinating seeds; conducting gravity field mutagenizing; conducting directional subculture breeding; collecting obtained woad seeds to be subjected to seed germination, obtaining woad buds and dividing the woad buds into two groups A and B, conducting gamma ray source irradiation treatment on the buds in the group A, using a colchicine solution for performing chromosome doubling treatment on the buds in the group A, and screening out tetraploid buds; obtaining diploid buds in the group B; conducting transplanting treatment on the buds in the group A and the buds in the group B and then obtaining group A tetraploid plants and group B diploid plants respectively; adopting the group A tetraploid plants as a female parent, adopting the group B diploid plants as a male parent, conducting sexual hybridization, and obtaining woad triploid plants.The obtained triploid plants have the dual advantages of polyploids and hybrids and are highly sterile, the hybrid vigor is fixed, the genetic characters can be continuously inherited through a maintainer line, and the problem of posterity segregation is avoided.

Description

technical field [0001] The invention relates to the field of breeding of Isatis indigo, in particular to a triploid breeding method of Isatis indigo with obvious rhizome weight gain. Background technique [0002] Isatis indigo is a commonly used Chinese medicinal material, and the whole plant can be used as human medicine. The root of the Chinese medicine is called Banlangen, which has the effects of clearing away heat, cooling blood and detoxifying. It is mainly used clinically to treat various diseases such as influenza, mumps, Japanese encephalitis, and hepatitis. The traditional Chinese medicine of its leaves is called Daqingye, which contains indigo and indirubin. In recent years, indirubin has been found to have the effect of treating chronic myelogenous leukemia. Therefore, cultivating woad has high economic value and medicinal value. [0003] In order to increase the content of medicinal ingredients in Isatis Radix, in recent years, researches on breeding methods o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/008A01H1/08A01H4/001
Inventor 周常
Owner 周常
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