Woad triploid breeding method with obvious rhizome weight increase
A technology of isatis and rhizomes, applied in the field of isatis triploidy breeding, can solve the problems of low survival rate, low mutation rate of breeding methods, huge consumption of manpower and material resources, etc., and achieves high nutritional value, rich yield and good applicability Effect
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Embodiment 1
[0031] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain
[0032] Include the following steps:
[0033] Step 1, seed germination: select Isatis indica seeds, wash them three times with sodium hypochlorite: chlorine dioxide as 5:1 sterile water, then soak them in hot water at 35°C for 1 hour, remove them, and transplant them into the liquid nutrient medium. Under the condition that the temperature is 25°C and the rotation speed of the medium is 15r / min, dark culture is carried out. After germination, the temperature is 28°C and the light intensity is 2500lx. bud. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 300mg / L, K 2 SO 4 , 100mg / L, KNO 3 , 120mg / L, (NH 4 ) 2 HPO 4 , 150mg / L, CaCl 2 , 50mg / L, ZnSO 4 , 50mg / L, NaCl, 15mg / L, MgCl 2 , 80mg / L, FeCl 2 , 20mg / L, Mg(BO 2 ) 2 , 180mg / L, vitamin A, 20mg / L, folic acid, 10mg / L, niacinamide, 50mg / L, vitamin E, 20mg / L, phenylpropanamide, 100mg / L, cysteine...
Embodiment 2
[0040] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain
[0041] Include the following steps:
[0042] Step 1, seed germination: select Isatis indica seeds, wash them with sodium hypochlorite: chlorine dioxide 1:1 disinfectant water for 5 times, then soak them in hot water at 50°C for 2 hours, remove them, and transplant them into the liquid nutrient medium. Under the condition that the temperature is 30°C and the rotation speed of the medium is 30r / min, dark culture is carried out. After germination, the temperature is 33°C and the light intensity is 3200lx. bud. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 500mg / L, K 2 SO 4 , 150mg / L, KNO 3 , 180mg / L, (NH 4 ) 2 HPO 4 , 250mg / L, CaCl 2 , 80mg / L, ZnSO 4 , 100mg / L, NaCl, 50mg / L, MgCl 2 , 100mg / L, FeCl 2 , 50mg / L, Mg(BO 2 ) 2 , 300mg / L, vitamin A, 50mg / L, folic acid, 30mg / L, niacinamide, 150mg / L, vitamin E, 60mg / L, phenylpropanamide, 180mg / L, cy...
Embodiment 3
[0049] A method for triploid breeding of Isatis indigo with obvious rhizome weight gain
[0050] Include the following steps:
[0051] Step I, seed germination: select Isatis indica seeds, wash them with sodium hypochlorite: chlorine dioxide 3:1 disinfectant water for 4 times, then soak them in hot water at 43°C for 1.5 hours, remove them, and transplant them into liquid nutrient medium , under the conditions of a temperature of 28°C and a rotation speed of the medium of 23r / min, dark culture was carried out. After germination, the temperature was 31°C and the light intensity was 2850lx. Woad sprouts. The composition and concentration of the liquid nutrient medium are: Co(NH 2 ) 2 , 350mg / L, K 2 SO 4 , 130mg / L, KNO 3 , 150mg / L, (NH 4 ) 2 HPO 4 , 230mg / L, CaCl 2 , 60mg / L, ZnSO 4 , 70mg / L, NaCl, 30mg / L, MgCl 2 , 90mg / L, FeCl 2 , 30mg / L, Mg(BO 2 ) 2 , 270mg / L, vitamin A, 30mg / L, folic acid, 25mg / L, niacinamide, 120mg / L, vitamin E, 35mg / L, phenylpropanamide, 140mg / L...
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