Preparation method of rape pollen polysaccharide
A technology of rape pollen polysaccharide and rape pollen, which is applied in the field of preparation of rape pollen polysaccharide, can solve the problems of high solvent consumption, unstable activity, and many extraction times, and achieve the effects of reducing energy consumption, saving raw materials, and using less
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[0027] Example 1 Selection of extraction process conditions
[0028] (1) The effect of the time of wall breaking and decomposition by ultrafine grinding method on the extraction effect of rape pollen polysaccharide
[0029] Experimental method: Take 500g of rape pollen and decompose it by ultrafine crushing method, put it in a plant extraction tank, add 60% ethanol as the extraction solvent, the weight-volume ratio of pollen to solvent is 1:15, and the extraction temperature is 65℃. Choose different The decomposition time is extracted once, the extract after decomposition and extraction is filtered by suction or centrifugation, the clear liquid is concentrated, and the thick paste is obtained after dichloromethane extraction, and dried to obtain the rape pollen extract. The extraction effect of rape pollen polysaccharide under different extraction time was investigated. The results are shown in Table 1:
[0030] Table 1
[0031] Time extraction rate (%)
[0032] 10h24.2
[0033] 14h30...
Example Embodiment
[0076] Example 2
[0077] Take 500g of rape pollen from the ultra-micro nano water mill and place it in a plant extraction tank. The ethanol concentration is 60%, the weight-volume ratio of the medicinal materials and the solvent is 1:15, the extraction temperature is 60℃, and the extraction time is 120min. The extract after extraction After suction filtration or centrifugation, the clear liquid is concentrated and extracted with dichloromethane to obtain a thick paste. Ethanol is added for C18-preparative chromatographic separation to obtain a rape pollen extract thick paste, which is dried to obtain rape pollen polysaccharide. Results: The dry paste yield was 6.5%, the content was 25.0%, and the extraction rate was 99.7%.
Example Embodiment
[0078] Example 3
[0079] As shown in Example 2, the difference is that the weight-volume ratio of pollen to solvent in Example 2 is 1:20, and the weight-volume ratio of pollen to solvent in Example 3 is 1:10. Results: The yield of dry paste was 5.9%, the content was 16.7%, and the extraction rate was 91.7%.
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