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Root specific expression AhOda promoter and application thereof

A root-specific promoter technology, applied in the fields of application, recombinant DNA technology, angiosperms/flowering plants, etc., can solve the problem of rare reports of peanut root-specific promoters

Active Publication Date: 2016-06-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many root-specific promoters have been cloned, and their sources include tobacco, rice, corn, Arabidopsis, tomato, soybean, etc., but reports of peanut root-specific promoters are relatively rare

Method used

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  • Root specific expression AhOda promoter and application thereof
  • Root specific expression AhOda promoter and application thereof
  • Root specific expression AhOda promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Experimental materials

[0035] 1.1 Plant material

[0036] The cultivated peanut (Arachishypogaea L.) variety Baisha 1016 was provided by Shandong Peanut Research Institute.

[0037] Tobacco (Nicotianabenthamiana), preserved by our laboratory.

[0038] 1.2 Strains and plasmids

[0039] The strains and plasmids used in this study are listed in Table 1. The following strains and plasmids are available for distribution to the public.

[0040] Table 1 Strains and plasmids

[0041]

[0042] 1.3 Database and biological software

[0043] 1, 3, 1 database

[0044] NCBI: http: / / www.ncbi.nlm.nih.gov /

[0045] 1, 3, 2 biological software

[0046] DNAMAN6.0: Sequence Alignment Analysis Software

[0047] Primer5.0: Primer Design Software

[0048] 1, 4 Reagents

[0049] 1, 4, 1 RNA extraction, reverse transcription and fluorescent quantitative PCR reagents

[0050] TotalRNAExtractor for RNA extraction was purchased from Shanghai Bioengineering Company, other RNasef...

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Abstract

The invention relates to a root specific expression AhOda promoter and application thereof and belongs to the technical field of biology. The nucleotide sequence of the root specific expression AhOda promoter is shown as SEQ ID NO1 or SEQ ID NO2 or SEQ ID NO3. Peanut root specific promoters are cloned, core areas of the promoters are determined through a truncation experiment, the functions of all the promoters are verified, and a foundation is laid for application and development of the peanut biotechnology of China. An important molecular basis is provided for providing the efficient, stable and special expression promoter for obtaining a bivalent grub resisting peanut variety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a root-specific promoter, its cloning, functional verification and its application. Background technique [0002] Cultivated peanut (Arachishypogaea L.) belongs to leguminous crops and is an important oil crop and economic crop in the world. In 2015, my country's peanut production reached 16.7 million tons, ranking first in the world. The yield of peanut is affected by some biotic and abiotic stresses, especially soil pests, fungi, bacteria, etc. In recent years, the occurrence of underground pests in peanut fields has been rampant, seriously affecting the yield and quality of peanuts, resulting in a large area of ​​peanut production reduction. [0003] Subterranean pests refer to omnivorous pests that harm the main stems, roots, and seeds near the soil surface of plants at a certain stage in their life history, causing damage to plant roots, affecting plants' absorption of water an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N9/0071C12N15/8227C12N15/8286C12Y114/11012
Inventor 耿丽丽张杰冯艳芳束长龙彭琦宋福平梁影屏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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