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A kind of assay method of oleanolic acid in Clematis clematis

A determination method and technology of oleanolic acid, applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills and complex pharmacopoeia operation process, and achieve the effects of good repeatability, consistent accuracy and simple operation.

Active Publication Date: 2017-11-24
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • A kind of assay method of oleanolic acid in Clematis clematis
  • A kind of assay method of oleanolic acid in Clematis clematis
  • A kind of assay method of oleanolic acid in Clematis clematis

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Embodiment 1

[0036] Grind the clematis through a pulverizer, pass through a No. 3 sieve, about 1g, weigh it accurately, mix it with an appropriate amount of quartz sand, and set aside for use. Add 1g of diatomaceous earth to the 10ml extraction pool first, then add the sample that is evenly mixed, add an appropriate amount of quartz sand after mixing evenly, shake gently to make it 2mm below the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction (including 2 steps of impurity removal and extraction, see ASE condition method), evaporate to dryness on a water bath, add 30ml of 2mol / L hydrochloric acid solution to the residue to dissolve, and heat to reflux for 2 hours. Cool immediately, transfer to a separatory funnel, wash the container with 10ml of water, and put the washing liquid into the separatory funnel. Add ethyl acetate and shake and extract 3 times, 15ml each time, combine the ethyl acetate solution, concentrate to near dryness b...

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Abstract

The invention discloses a method for determining oleanolic acid in Clematis clematis, which comprises the following steps: Step 1: crushing Clematis clematis and extracting it with an ASE extraction method to obtain an extract; Step 2: centrifuging the extract, Obtain the supernatant; step 3: analyze the supernatant by liquid chromatography. The invention provides a method for determining oleanolic acid in Clematis clematis, which is simple in operation and high in detection accuracy.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to a method for determining oleanolic acid in Clematis clematis. Background technique [0002] In 2015, the Pharmacopoeia recorded the method of extracting oleanolic acid in Clematis clematis: take about 4g of the powder of this product (passed through a No. 4 sieve), accurately weigh it, put it in a Soxhlet extractor, add an appropriate amount of ethyl acetate, and heat to reflux for 3 hour, discard the ethyl acetate solution, evaporate the solvent from the dregs, transfer it to a conical flask together with the filter paper cylinder, add 50ml of dilute ethanol precisely, weigh it, heat and reflux for 1 hour, let it cool, weigh it again, and use dilute Make up the lost weight with ethanol, shake well, filter, accurately measure 25ml of the filtrate, put it on a water bath and evaporate to dryness, add 30ml of 2mol / L hydrochloric acid solution to the residue to dissolve, and heat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/74G01N30/06
CPCG01N30/06G01N30/74
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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