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Application of proteins and their related biomaterials in improving plant yield
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A biological material and protein technology, applied in the biological field, can solve problems such as the reduction of arable land area and the limited potential for increasing production of bred varieties
Inactive Publication Date: 2019-04-09
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
At present, the area of arable land is gradually decreasing, and the potential for increasing production of the current bred varieties is limited. The world is facing a new round of food security challenges. Therefore, it is necessary to develop new varieties with greater potential for increasing production.
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Embodiment 1
[0076] Example 1. Functional verification of TaSPL20-7D gene
[0077] The total RNA of wheat (Triticum aestivum L.) variety Yanzhan 4110 seedlings was extracted with TRIZOL, and the first-strand cDNA (Invitrogen) was synthesized with the M-MLV reverse transcription kit. The cDNA was used as a template, and primers F1 and R1 were used for PCR amplification , a PCR amplification product of 1445bp was obtained.
[0078] F1: 5'-AAGAGTTATTGCCAACGGAC-3' (positions 1-20 of sequence 1);
[0079] R1: 5'-CTAATGTGGTGTTCTAACTGTGG-3' (reverse complement of positions 1423-1445 of SEQ ID NO: 1).
[0080] After sequencing, the sequence of the PCR product is shown in sequence 1 in the sequence table, the 213-1379 nucleotides of sequence 1 are the coding region, and the gene shown in the 213-1379 nucleotides of sequence 1 is named as TaSPL20-7D gene, the protein encoded by this gene is named TaSPL20-7D, and its amino acid sequence is sequence 2 in the sequence table.
[0081] First, the acqu...
Embodiment 2
[0116] Example 2. Expression of TaSPL20-7D gene in different tissues
[0117] 1. Expression of TaSPL20-7D gene in different tissues
[0118] Different tissue parts were taken as samples at different growth stages of wheat (Triticum aestivum L.) variety Yanzhan 4110, frozen in liquid nitrogen, and stored at -80°C for future use. The sample materials obtained include: seedling stage root (SR), seedling stage apical meristem (SC), 4-leaf stage apical meristem (4C), 6-leaf stage apical meristem (6C), and turning green stage apical meristem (GC)), seedling leaf (SL), root (BR), node (BN), internode (BI), leaf sheath (BS), leaf (BL), ear (BE) after booting (0.5cm ear ( 0.5E), 2cm ear (2E), 3cm ear (3E), 6cm ear (6E), 8cm ear (8E), 10cm ear (10E)), ear in late booting (BE), ear in flowering stage (FE) , 4 days after anthesis (4G), 7 days after anthesis (7G), 14 days after anthesis (14G) and 21 days after anthesis (21G).
[0119] TRIZOL was used to extract the total RNA of the samp...
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Abstract
The invention discloses application of a protein and a related biological material thereof in increasing plant yield. The protein is the following A1) or A2) or A3): A1) protein of which the amino acid sequence is Sequence 2; A2) plant-yield-related protein obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence disclosed as Sequence 2 in the sequence table; or A3) fusion protein obtained by connecting a tag to the N terminal or / and C terminal of A1) or A2). The increase of the plant yield comprises improvement of plant yield related traits, including at least one of spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number. The experiment proves that the protein and coding gene thereof can be utilized to enhance the spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number of the plants, thereby increasing the plant yield.
Description
technical field [0001] The invention relates to the application of proteins and related biological materials in the field of biotechnology in improving plant yield. Background technique [0002] The production and output of grain is not only related to people's daily life, but also restricts the economic situation of the country to a certain extent. As the country with the largest population in the world, it is particularly important to increase the output of grain. In the past few decades, grain production has increased significantly. In addition to the improvement of farming and management conditions, the genetic improvement of crop varieties has played an important role in increasing grain production. However, the increase in grain production in recent years has reached a bottleneck period. At present, the area of arable land is gradually decreasing, and the current bred varieties have limited yield increase potential. The world is facing a new round of food security ...
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