Preparation method of SIS tissue repair material and application of preparation method

A tissue repair, S03 technology, applied in tissue regeneration, medical science, prosthesis and other directions, can solve problems such as endangering the health of operators, reducing SIS repair ability, and inactivating growth factors, achieving complete degreasing, enhancing degreasing effect, Restorative effect

Inactive Publication Date: 2016-07-06
苏州期佰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation methods of SIS are mostly chemical methods, in which a large amount of organic solvents, surfactants and enzymes are mostly used. These substances have certain toxicity to growth factors, inactivate growth factors in SIS, destroy or reduce Repair ability
In addition, organic solvents such as benzene and chloroform are often used in chemical methods, which not only makes the SIS preparation method highly toxic, endangers the health of operators, but also brings serious pollution to the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method for SIS tissue repair material, characterized in that: comprising the following steps:

[0026] S01, take the small intestine, scrape off the jejunum serosa layer, mucous membrane layer and muscular layer, wash with deionized water, and cut it to obtain the small intestine submucosa;

[0027] S02, degreasing: Put the small intestinal submucosa in S01 into the diafiltration cartridge, add leachate equivalent to 3 times the weight of the small intestinal submucosa and soak for 6 hours, start diafiltration after soaking, and add infiltrate 20 times the weight of the small intestinal submucosa Filtrate, the percolation rate is 1ml / min, and the percolate is a mixed solution of ethanol and surfactant;

[0028] S03, cleaning the degreased small intestinal submucosa with deionized water;

[0029] S04, decellularization: the small intestinal submucosa washed in S03 was digested with trypsin, and rinsed with deionized water after digestion;

[0030] S05, de...

Embodiment 2

[0040] A preparation method for SIS tissue repair material, characterized in that: comprising the following steps:

[0041] S01, take the small intestine, scrape off the jejunum serosa layer, mucous membrane layer and muscular layer, wash with deionized water, and cut it to obtain the small intestine submucosa;

[0042] S02, degreasing: Put the small intestinal submucosa in S01 into the diafiltration cartridge, add leachate equivalent to 5 times the weight of the small intestinal submucosa and soak for 8 hours, start infiltrating after soaking and add diafiltration liquid equivalent to 30 times the weight of the small intestinal submucosa Filtrate, the diafiltration rate is 2ml / min, and the diafiltration liquid is the mixed solution of ethanol and tensio-active agent;

[0043] S03, cleaning the degreased small intestinal submucosa with deionized water;

[0044] S04, decellularization: the small intestinal submucosa washed in S03 was digested with trypsin, and rinsed with deio...

Embodiment 3

[0055] A preparation method for SIS tissue repair material, characterized in that: comprising the following steps:

[0056] S01, take the small intestine, scrape off the jejunum serosa layer, mucous membrane layer and muscular layer, wash with deionized water, and cut it to obtain the small intestine submucosa;

[0057] S02, degreasing: Put the small intestinal submucosa in S01 into the diafiltration cartridge, add leachate equivalent to 4 times the weight of the small intestinal submucosa and soak for 10 hours, start diafiltration after soaking, and add diafiltration fluid equivalent to 25 times the weight of the small intestinal submucosa Filtrate, the percolation rate is 1.5ml / min, and the percolate is a mixed solution of ethanol and surfactant;

[0058] S03, cleaning the degreased small intestinal submucosa with deionized water;

[0059] S04, decellularization: the small intestinal submucosa washed in S03 was digested with trypsin, and rinsed with deionized water after dige...

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PUM

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Abstract

The invention discloses a preparation method of an SIS tissue repair material and application of the preparation method. The preparation method of the SIS tissue repair material is characterized by comprising the following steps that 1, a small intestine is taken, a serosa layer, a mucosal layer and a muscular layer of jejunum are scraped away, and after the treated small intestine is cleaned with deionized water and cut off, small intestinal submucosa is obtained; 2, degreasing is conducted, the small intestinal submucosa obtained in the first step is put into a percolation tube, percolate with the weight 3-5 times of that of the small intestinal submucosa is added, soaking is conducted for at least 6 h, after soaking is completed, percolation is conducted, and percolate with the weight 20-30 times of that of the small intestinal submucosa is added, wherein the percolation speed is 1-2 ml/min, and the percolate is a mixed solution of ethyl alcohol and surfactant. According to the preparation method of the SIS tissue repair material and the application of the preparation method, the preparation method is free of toxicity and environmentally friendly, and the SIS repair capacity is high.

Description

technical field [0001] The invention relates to a preparation method and application of an SIS tissue repair material, belonging to the technical field of biomaterials. Background technique [0002] Small intestinal submucosa (smallintestinesubmucosa, SIS), one of the components of the small intestine, is a biodegradable natural extracellular matrix-like biologically derived material that contains a variety of growth factors, which play a role in tissue repair and reconstruction. important facilitation. [0003] At present, the preparation methods of SIS are mostly chemical methods, in which a large amount of organic solvents, surfactants and enzymes are mostly used. These substances have certain toxicity to growth factors, inactivate growth factors in SIS, destroy or reduce repair ability. In addition, organic solvents such as benzene and chloroform are often used in chemical methods, which not only makes the SIS preparation method highly toxic, endangers the health of op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3629A61L27/54A61L2300/252A61L2300/414A61L2430/34A61L2430/40
Inventor 韩立军
Owner 苏州期佰生物技术有限公司
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