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A kind of preparation method of gelatin/carbonated hydroxyapatite bone scaffold

A technology for carbonating hydroxyapatite and hydroxyapatite, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of difficult to achieve mechanical support mechanical requirements, difficult to meet industrial production, complicated steps, etc. The effect of good medical application prospect, easy industrialization and simple preparation process

Inactive Publication Date: 2019-01-08
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Some of the currently published patents use simple blending methods to prepare biomimetic ceramic materials. Although the structure is close to natural bone to a certain extent, the texture is usually brittle and difficult to meet the mechanical requirements of mechanical support; The material is close to natural bone in terms of structure, biological properties and mechanical properties, and various improvements have been made. Although beneficial effects have been achieved to a certain extent, there may be more substances to be added, or the steps are cumbersome, and it is difficult to meet the requirements of the industry. production requirements

Method used

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  • A kind of preparation method of gelatin/carbonated hydroxyapatite bone scaffold
  • A kind of preparation method of gelatin/carbonated hydroxyapatite bone scaffold
  • A kind of preparation method of gelatin/carbonated hydroxyapatite bone scaffold

Examples

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Effect test

Embodiment 1

[0066] A method of preparing gelatin / carbonated hydroxyapatite bone scaffold:

[0067] (1) Preparation of gelatin / hydroxyapatite suspension: disperse nano-hydroxyapatite (length 100nm, diameter 20nm) into distilled water, ultrasonically disperse the hydroxyapatite nanoparticles, the mass concentration is 5%. Then it was heated to 40° C., gelatin was added while stirring, and the stirring was continued to completely dissolve the gelatin to obtain a gelatin / hydroxyapatite suspension with a gelatin mass concentration of 15%.

[0068] (2) Preparation of physically cross-linked gelatin / hydroxyapatite hydrogel: pour the gelatin / hydroxyapatite suspension into a mold, and quickly place it in a refrigerator at 1° C. to induce physical gelation. After 12 hours, the gelatin / hydroxyapatite hydrogel that had been gelled was taken out of the mold to obtain a physically cross-linked gelatin / hydroxyapatite hydrogel.

[0069] (3) Preparation of chemically cross-linked gelatin / hydroxyapatite h...

Embodiment 2

[0072] A method of preparing gelatin / carbonated hydroxyapatite bone scaffold:

[0073] (1) Preparation of gelatin / hydroxyapatite suspension: disperse nano-hydroxyapatite (length: 200nm, diameter: 50nm) into distilled water, ultrasonically disperse the hydroxyapatite nanoparticles, the mass concentration is 8%. Then it was heated to 45° C., gelatin was added while stirring, and the stirring was continued to completely dissolve the gelatin to obtain a gelatin / hydroxyapatite suspension with a gelatin mass concentration of 20%.

[0074] (2) Preparation of physically cross-linked gelatin / hydroxyapatite hydrogel: pour the gelatin / hydroxyapatite suspension into a mold, and quickly place it in a refrigerator at 3° C. to induce physical gelation. After 12 hours, the gelatin / hydroxyapatite hydrogel that had been gelled was taken out of the mold to obtain a physically cross-linked gelatin / hydroxyapatite hydrogel.

[0075] (3) Preparation of chemically crosslinked gelatin / hydroxyapatite ...

Embodiment 3

[0078] A method of preparing gelatin / carbonated hydroxyapatite bone scaffold:

[0079] (1) Preparation of gelatin / hydroxyapatite suspension: disperse nano-hydroxyapatite (length: 300nm, diameter: 100nm) into distilled water, ultrasonically disperse the hydroxyapatite nanoparticles, the mass concentration is 10%. Then it was heated to 55° C., gelatin was added while stirring, and the stirring was continued to completely dissolve the gelatin to obtain a gelatin / hydroxyapatite suspension with a gelatin mass concentration of 40%.

[0080] (2) Preparation of physically cross-linked gelatin / hydroxyapatite hydrogel: pour the gelatin / hydroxyapatite suspension into a mold, and quickly place it in a refrigerator at 1° C. to induce physical gelation. After 12 hours, the gelatin / hydroxyapatite hydrogel that had been gelled was taken out of the mold to obtain a physically cross-linked gelatin / hydroxyapatite hydrogel.

[0081] (3) Preparation of chemically crosslinked gelatin / hydroxyapatit...

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Abstract

The invention discloses a preparation method of a gelatin / carbonated hydroxyapatite bone scaffold.The preparation method includes the steps of firstly, dispersing hydroxyapatite into distilled water, and adding gelatin to prepare gelatin / hydroxyapatite suspension; secondly, pouring the suspension into a required die, and cooling in a refrigerator to prepare physically-crosslinked gelatin / hydroxyapatite hydrogel; thirdly, placing the physically-crosslinked gelatin / hydroxyapatite hydrogel into a crosslinking solution for chemical crosslinking; fourthly, placing the chemically-crosslinked gelatin / hydroxyapatite hydrogel into modified simulated body fluid, culturing, and performing freeze drying.The prepared gelatin / carbonated hydroxyapatite bone scaffold has the composition and structure similar to the bone and is good in biological and mechanical performance, low in preparation cost and promising in application prospect.

Description

technical field [0001] The invention relates to the technical field of preparation of artificial bone scaffold materials, in particular to a method for preparing a gelatin / carbonated hydroxyapatite bone scaffold. Background technique [0002] Severe bone injuries caused by car accidents, fatigue sports, etc., or bone defects and nonunions caused by tumors, inflammations, and various deformities need to be effectively repaired by bone grafting. [0003] With the development of science and technology, there are many options for the source of bone graft materials. Autologous heterotopic bone materials, allogeneic bone materials, xenogeneic bone materials and artificially synthesized bionic bone materials have undergone a lot of experimental and clinical research in bone transplantation, and have been practically applied in clinical practice. However, these materials have many defects in practical applications and cannot be used as a good substitute for natural bone. [0004] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/50
CPCA61L27/46A61L27/50A61L2430/02C08L89/06
Inventor 韩志超张子鑫许杉杉
Owner INST OF CHEM CHINESE ACAD OF SCI