Method for improving blood sodium valproate detection sensitivity

A technology of detection sensitivity and sodium valproate, which is applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of difficult development in primary medical institutions and high cost of fluorescence polarization immunoanalyzers, and achieves fast measurement speed, The effect of significant curative effect and simple measurement method

Active Publication Date: 2016-07-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Fluorescence polarization immunoassay, fluorescence polarization immunoassay mainly relies on the TDx-FLx fluorescence polarization immunoassay analyzer, after taking the serum, use Abbott’s sodium valproate kit to complete the determination of blood drug concentration easily and quickly, but the TDxFLx fluorescence polarization immunoassay The cost of polarization immunoanalyzer is high, and it is difficult for primary medical institutions to carry out

Method used

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  • Method for improving blood sodium valproate detection sensitivity
  • Method for improving blood sodium valproate detection sensitivity
  • Method for improving blood sodium valproate detection sensitivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Dissolve the valproic acid standard in an organic solvent (alcohol), use a series of valproic acid concentrations of 10mg / L, 20mg / L, 40mg / L, 100mg / L and 200mg / L as the standard, take 10-100μL standard The sample is dropped on the sample chip, and the ion mobility spectrometer is used to detect and analyze the negative ion high pressure mode. After the sample is thermally desorbed, the migration time and the maximum signal peak intensity of the ion reaching the ion mobility spectrometer are measured.

[0032] figure 2 In the ion mobility spectrogram of the thermal analysis signal of valproic acid in negative ion mode, the RIP signal is at 4.3ms, and the migration time of the valproic acid signal is 7.3ms and 9.5ms. The quantitative analysis of the embodiment of the present invention selects the characteristic peak of 7.3ms.

Embodiment 2

[0034] Within the scope of quantitative analysis concentration 10-200mg / L, get valproic acid concentration and be respectively 10mg / L, 20mg / L, 40mg / L, 100mg / L, 200mg / L, according to detection method of the present invention, same concentration detects three times The average value of the signal is 67mv, 77mv, 104mv, 155mv, 254mv in turn, and the obtained marking equation is y=0.973x+59.3997, such as image 3 shown.

Embodiment 3

[0036] Take 10-100 μL of blood samples to be tested from people or animals who are injected or taken with sodium valproate, drop them on the sample insert, add alcohol additives at the same time, use ion mobility spectrometers, use negative ion high pressure mode detection and analysis, and thermal analysis After the sample, measure the analytical signal tracking trend line of the same ion arriving at the ion mobility spectrometer detector to determine the maximum signal peak intensity; according to the maximum signal peak intensity, bring it into the standard curve equation to calculate the blood concentration of valproic acid.

[0037] Such as Figure 4 , the maximum signal peak intensity value of the tracking trend line is brought into the fitting marking equation, and the blood drug concentration can be calculated as 40mg / L.

[0038] The established qualitative and quantitative analysis methods meet the concentration analysis range (50-100mg / L) for the human body administe...

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Abstract

The invention discloses a method for improving blood sodium valproate detection sensitivity. The method utilizes ion mobility spectrometry as a basic detection technology, utilizes an ion mobility spectrometer as detection means and utilizes a negative ion high pressure mode, a thermo-desorption sample introduction method and a complex sample-free pre-treatment method. A device used in the method is stable and reliable. The method is simple, fast and efficient. Detection and analysis time of a single sample is less than 1min. The method has high detection sensitivity, a detection limit of 1mg/L and a quantitative analysis concentration scope of 10-200mg/L. The built qualitative and quantitative analysis method satisfies a human body clinical administration concentration analysis scope of 50-100mg/L of epilepsy patients. The detection method can be widely used for clinical administration deep analysis and provides guidance for clinical accurate administration by doctors.

Description

technical field [0001] The invention discloses a method for improving the detection sensitivity of sodium valproate in blood. The method uses ion mobility spectrometry as the basic detection technology, uses ion mobility spectrometer as the detection means, adopts negative ion high pressure mode, thermal analysis sample injection method, and has no complicated sample pretreatment method. The device in the invention is stable and reliable, and the method is simple, fast and efficient. Background technique [0002] Sodium valproate is an antiepileptic drug, and its mechanism of action has not been fully elucidated. Experiments show that this product can increase the synthesis of GABA and reduce the degradation of GABA, thereby increasing the concentration of inhibitory neurotransmitter γ-aminobutyric acid (GABA), reducing the excitability of neurons and inhibiting seizures. In the electrophysiological experiment, it was seen that this product can inhibit Na + The role of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/622
Inventor 王新周庆华渠团帅李海洋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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