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Application of Unsaturated Fatty Acid Metabolites in Seminal Plasma

A technology for unsaturated fatty acids and metabolites, which is applied in the application field of unsaturated fatty acid metabolites in seminal plasma, can solve the problems of poor stability and repeatability of results, short half-life of active oxygen, etc., and achieve good economical effects

Active Publication Date: 2019-07-19
上海市生物医药技术研究院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, a large number of clinical samples and laboratory studies have found that the half-life of active oxygen is short, and there are abundant antioxidant enzymes in the seminal plasma to continuously remove active oxygen. In addition, active oxygen also participates in the polyunsaturated fatty acids rich in sperm plasma membrane Lipid peroxidation chain reaction occurs, these factors lead to poor stability and repeatability of the results obtained by direct detection of active oxygen in semen, so direct detection of active oxygen is not a good quantitative evaluation index of oxidative stress

Method used

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  • Application of Unsaturated Fatty Acid Metabolites in Seminal Plasma
  • Application of Unsaturated Fatty Acid Metabolites in Seminal Plasma
  • Application of Unsaturated Fatty Acid Metabolites in Seminal Plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Example 1 Correlation Study on Unsaturated Fatty Acid Metabolites in Seminal Plasma and Fertility Status

[0024] From the seminal plasma of 761 participants in the Guizhou male fertility health survey cohort (including 517 cases of fertile men and 244 cases of infertile men), the correlation between the content of unsaturated fatty acid metabolites in seminal plasma and their fertility status was studied. The following results are obtained:

[0025] There were significant differences in the contents of 8 unsaturated fatty acid metabolites in seminal plasma between fertile and infertile men. These metabolites include: 15dPGJ 2 , 5,6-DHET, 11,12-DHET, 17(S)HDOHE, 15-HETE, 20-HETE, 7-MARESIN and PGJ 2 .

[0026] For these 8 seminal plasma unsaturated fatty acid metabolites with significant differences in the content of fertile men and infertile men, the researchers used Logistic regression model to carry out further analysis after adjusting the confounding factors of ag...

Embodiment 2

[0030] Example 2 In vitro sperm oxidative damage culture model and the correlation between sperm metabolites and oxidative damage and sperm motility

[0031] 1. In vitro sperm oxidative damage culture model, as shown in Table 2.

[0032] Table 2 Sperm culture model of oxidative damage in vitro

[0033]

[0034]

[0035] 2. Merge the changes in different time periods in Table 2 into three groups, namely the control group, the low-concentration active oxygen group and the high-concentration active oxygen group, see Table 3.

[0036] Table 3 shows the control group, low concentration active oxygen group and high concentration active oxygen group of sperm in vitro oxidative damage culture model

[0037]

[0038] Conclusion: As the concentration of reactive oxygen species increased, 13-HODE and 9-HODE also increased, and the significance of the trend test was <0.0001.

[0039] 3. The correlation between 13-HODE and 9-HODE and sperm forward motility and survival rate was ...

Embodiment 3

[0044] Example 3 Correlation between unsaturated fatty acid metabolites in seminal plasma and semen quality

[0045] The correlation between the content of unsaturated fatty acid metabolites in seminal plasma and semen quality was studied from the seminal plasma of 761 participants in Guizhou male fertility health survey cohort (including seminal plasma of 517 cases of fertile men and 244 cases of infertile men). The following results are obtained:

[0046] We found that the levels of 14 metabolites were significantly correlated with different semen parameters (liquefaction time, semen volume, sperm density, fast forward motility, slow forward motility, normal morphology rate and non-sperm cell rate), see table 5. in:

[0047] Liquefaction time: Semen liquefaction refers to the change process of semen from the gel state at the time of ejaculation to the liquid state. Under normal circumstances, after the semen is ejaculated, put it in a constant temperature water bath at 37...

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Abstract

Relevance between the content levels of seminal plasma unsaturated fatty acid metabolites and fertility conditions, seminal fluid quality, active oxygen levels, the sperm forward movement capacity, the environmental internal-secretion interfering-substance level and the seminal plasma calcium and magnesium proportion is researched by detecting the content of unsaturated fatty acid metabolites existing in fertile and sterile male seminal plasma, and differences between the unsaturated fatty acid metabolites existing in fertile and sterile male seminal plasma are further verified. In view of the differences between unsaturated fatty acid metabolites existing in fertile and sterile male seminal plasma, the unsaturated fatty acid metabolites can be used for detecting markers of the fertility conditions, the seminal fluid quality, the active oxygen levels, the sperm forward movement capacity, the environmental internal-secretion interfering-substance level, the seminal plasma calcium and magnesium proportion and the like and can be used for measuring the effectiveness of male sterility medicine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of seminal plasma unsaturated fatty acid metabolites. Background technique [0002] In recent years, the incidence of male infertility symptoms has gradually increased. Worldwide, about 15%-20% of couples of childbearing age are infertile, and the male factor accounts for about 50%. Routine semen analysis, such as sperm count, viability, motility and morphology, is a common method for judging fertility. However, nearly 30% of male infertility patients have normal or close to normal semen analysis results. Therefore, there have been great difficulties in the clinical diagnosis of male infertility for a long time, mainly because routine semen analysis indicators such as sperm count, viability, motility and morphology can only reflect the most basic semen quality, but cannot reflect all sperm functions. Therefore, it is necessary to discover new biomarkers t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48A61K31/557A61K31/5575A61K31/558A61K31/201A61P15/08
CPCA61K31/201A61K31/557A61K31/5575A61K31/558G01N33/48G01N2800/34G01N2800/7004
Inventor 戴奇施惠娟尹慧勇
Owner 上海市生物医药技术研究院
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