Treatment method for bacterial contamination in tissue-culture industrialized seedling culture of tropical woody plants

A technology for woody plants and bacterial contamination, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as bacterial contamination, and achieve the effect of meeting market demand

Inactive Publication Date: 2016-08-03
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of bacterial contamination in tropical woody plant tissue culture industrialized seedling raising that solves the common bacterial contamination problem caused by artificial transfer in the process of woody plant tissue culture industrialized seedling raising by a kind of simple method processing method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In November 2013, the applicant obtained sterile vaccines for bacteria-contaminated Eucalyptus gigantea clone SP7 at the Tissue Culture Breeding Center of Tropical Forestry Research Institute, No. 682, Guangshan 1st Road, Guangzhou City, Guangdong Province, in November 2013. In March 2015, the multiplication seedlings of Eucalyptus urophylla G19 and 3213 were treated, and until June 2015, sterile seedlings were obtained.

[0032] The processing method of bacterial contamination in the industrialized seedling cultivation of tropical woody plant tissue culture, the specific steps are as follows:

[0033] S1、 Preparation of culture medium

[0034] Proliferation medium adopts mWPM medium + 6BA1.0mg.L -1 +IBA0.5mg.L -1 , Prepare antibiotics into 100mg / L Cef stock solution, and use filter sterilization to sterilize Cef or TMT. Take out the high-temperature sterilized proliferation medium from the sterilizer, add antibiotics when the temperature of the medium is lowered to...

Embodiment 2

[0047]In April 2015, the applicant treated the bacteria-contaminated seedlings of teak clone 7544 in the Tissue Culture and Breeding Center of Tropical Forestry Research Institute, No. 682, Guangshan 1st Road, Guangzhou City, Guangdong Province. By July 2015, the bacterial contamination was completely removed. Specific steps are as follows:

[0048] Cultivate the multiplied seedlings in the multiplication medium for 25 days, and select bottles with relatively few visible bacterial colonies to carry out the following tests. At the same time, the plants grow relatively well, and the bud height is 8-10cm. Proliferation medium adopts MS medium + 6BA2.0mg.L -1

[0049] The operations of the transfer and cultivation of the proliferating seedlings and the bacteriostatic treatment of the proliferating seedlings are the same as in Example 1. Among them, when the pointed tweezers are grasping, the top 1-2 internodes are 3-5cm long. In the comparative test, 150mg / L of cef inhibited th...

Embodiment 3

[0052] In April 2015, the applicant treated the bacteria-contaminated Acacia blackwood multiplication seedlings in the Tissue Culture and Breeding Center of Tropical Forestry Research Institute, No. 682, Guangshan 1st Road, Guangzhou City, Guangdong Province, and the bacterial contamination was completely removed by July 2015.

[0053] Specific steps are as follows:

[0054] Cultivate the multiplied seedlings for 25 days in the multiplication medium, select bottles with relatively few bacterial colonies visible to the naked eye to carry out the following tests, and the plants grow relatively well at the same time, with more clusters of buds, and select plants with a bud height of 3-5cm. Proliferation medium adopts MS medium + 6BA1.0mg.L -1 +IBA0.1mg.L -1 .

[0055] The operations of the transfer and cultivation of the proliferating seedlings and the bacteriostatic treatment of the proliferating seedlings are the same as in Example 1. Among them, when the pointed tweezers ar...

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Abstract

The invention provides a treatment method for bacterial contamination in tissue-culture industrialized seedling culture of tropical woody plants. The method comprises the following steps that antibiotics are selected, subjected to filtration sterilization and then added into a proliferation medium to prepare a medium; proliferation seedlings of the tropical woody plants are cultured in the medium, the growth state of the plants is relatively good, and the proliferation seedlings which have few visible bacterial colonies are selected for experiments; in a clean bench, 1 cm-2 cm of a plant stem tip is clamped with well-disinfected fine-tipped tweezers and transferred into the proliferation medium added with the antibiotics; subculture is conducted on the proliferation seedlings of the tropical woody plants multiple times according to the period. According to the treatment method for bacterial contamination in the tissue-culture industrialized seedling culture of the tropical woody plants, bacterial contamination which is difficult to avoid in industrialized seedling culture and is caused by manual operation is relieved, interference of bacterial contamination to the plants is relieved in a short time, sterile good seedlings are obtained again, the market requirement is met, technical support is provided for plant tissue culture, specially for the industrialized seedling culture of the tropical woody plants, and a novel approach and technical method are provided for preservation methods and modes of valuable materials.

Description

technical field [0001] The invention relates to the technical field of tissue culture, and is particularly suitable for the treatment method of bacterial pollution in the tissue culture of tropical woody plants in industrial seedling cultivation. Background technique [0002] In the process of plant tissue culture industrial production and seedling cultivation, a large number of inoculation workers are needed, and the skills and operation levels of the inoculation workers are uneven. The bacterial contamination caused by improper operation during the multiplication process mainly comes from the bacteria carried by the inoculation workers themselves. A large amount of bacterial contamination occurs during rooting. It affects the normal growth and reproduction of tissue culture seedlings, and the explants need to be replaced frequently, and in serious cases, the explants need to be collected again every year. Since it takes a long period from explant disinfection to normal pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 范春节曾炳山裘珍飞
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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