New application of KCNK2 gene

A gene and gene chip technology, which is applied in the fields of medical preparations containing active ingredients, determination/inspection of microorganisms, biochemical equipment and methods, etc. Difficulty in changing the organizational structure and other problems, to achieve the effect of simplifying the quantitative detection process, strong selectivity, and clear results

Inactive Publication Date: 2016-08-10
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also missed diagnoses. The reasons for the missed diagnosis of FNA can be summarized as follows: the puncture needle is too thin, and the sample volume of cytological examination is insufficient; it is related to the size of the thyroid tumor and the internal tissue conditions. The lower the diagnostic rate of clinical examination, especially for thyroid tumors with internal liquefaction, the puncture needle only draws out fluid after penetrating into the capsule, without tissue cells, which is meaningless for diagnosis; cytological diagnosis is most effective for diseases with prominent changes in cell morphology , but it is difficult for lesions that mainly change in tissue structure

Method used

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  • New application of KCNK2 gene
  • New application of KCNK2 gene
  • New application of KCNK2 gene

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Embodiment 1

[0031] The collection of embodiment 1 case

[0032] Samples from 5 cases of papillary thyroid carcinoma and 8 healthy controls were collected. The case samples were patients with papillary thyroid carcinoma confirmed by intraoperative frozen rapid pathology and postoperative paraffin slow pathology, which were confirmed by eight thyroid function tests and thyroid color Doppler ultrasound examination.

Embodiment 2

[0033] Example 2 High-throughput sequencing and analysis

[0034] use Reagent extracts sample RNA, and after RNA extraction, agarose gel electrophoresis is used to detect the extraction of RNA samples by NanoDrop1000 spectrophotometer. The sample requirements for RNA-seq sequencing: OD260 / OD280 is 1.8-2.2.

[0035] The HiSeq 2500 high-throughput sequencing platform of Illumina was used for high-throughput transcriptome deep sequencing, and the Fast-QC software was used to evaluate the quality of the sequencing data after sequencing, including the quality value distribution of bases, the position distribution of quality values, GC content, PCR duplication content, kmer frequency, etc. In the analysis of differential gene expression, according to the obtained FPKM value, the internationally recognized algorithm EBSeq was used for differential screening. Among them, when filtering, LOG 2 FC>1 or <-1, FDR<0.05. In order to better understand the functions of differentially expr...

Embodiment 3

[0036] Example 3 KCNK2 gene expression in papillary thyroid carcinoma group and control group

[0037] 1. Materials and methods

[0038] 1. Materials

[0039] During the period from May 2013 to November 2015, 45 cases of papillary thyroid carcinoma tissues and 18 cases of healthy control tissues were selected and grouped and numbered.

[0040] 2. Method

[0041] 2.1 Extraction of total RNA in papillary thyroid carcinoma group and control group

[0042] use Reagent extracts sample RNA, and the operation refers to the instructions, as follows:

[0043] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0044] ① Add Trizol and let stand at room temperature for 5 minutes;

[0045] ② Add 0.2ml chloroform to every ml Trizol, vibrate the centrifuge tube vigorously, mix well, and place it at room temperature for 5-10min;

[0046] ③ After high-speed cen...

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Abstract

The invention relates to new application of a KCNK2 gene, in particular to application of the KCNK2 gene and an expression product thereof to treatment of papillary thyroid carcinoma as tumor markers .In order to diagnose thyroid carcinoma more accurately and avoid missed diagnosis and misdiagnosis, tumor molecular markers are screened based on sequencing and data analysis, the result shows that the KCNK2 gene can be used for preparing a papillary thyroid carcinoma auxiliary diagnosis and treatment preparation, and quite good clinical application value is achieved.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, the present invention relates to a new application of the KCNK2 gene, and more specifically relates to the application of the KCNK2 gene and its expression product as a tumor marker in the diagnosis and treatment of papillary thyroid cancer. Background technique [0002] Papillary carcinoma is a clinically common type of thyroid cancer, accounting for about 90% of all thyroid cancers. Diagnosis of thyroid cancer mainly relies on ultrasonography, which is one of the most valuable methods for diagnosing the primary lesion of thyroid cancer. Ultrasound examination also has a certain rate of misdiagnosis. Clinicians should understand the ultrasound manifestations of special types of thyroid cancer, such as cystic papillary thyroid carcinoma and diffuse papillary thyroid carcinoma, which are easily confused with thyroid cyst or lymphocytic thyroiditis clinically. . Fine-needle aspira...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/577G01N33/574G01N33/558A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00C12Q2600/158G01N33/558G01N33/57407G01N33/57484G01N33/577G01N33/68G01N2333/47
Inventor 李曙光杨承刚边洋
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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