A hybrid hydrogel based on chemical crosslinking of polyester nanoparticles and its preparation method and application

A nanoparticle and hydrogel technology, which is applied in the field of hybrid hydrogel and its preparation, can solve the problems of difficult chemical cross-linking formation and less active groups, and achieve good biodegradability and biocompatibility Effect

Active Publication Date: 2018-03-27
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polyester materials have fewer active groups and are difficult to meet the conditions for chemical crosslinking to form gels. Therefore, it is necessary to modify the surface of polyester materials to endow more active groups.

Method used

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  • A hybrid hydrogel based on chemical crosslinking of polyester nanoparticles and its preparation method and application
  • A hybrid hydrogel based on chemical crosslinking of polyester nanoparticles and its preparation method and application
  • A hybrid hydrogel based on chemical crosslinking of polyester nanoparticles and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1. Changes in the surface potential of nanoparticles at different treatment times

[0064] Follow the steps below to prepare ammonia-treated nanoparticles:

[0065] (1) Dissolve branched polyethyleneimine (b-PEI, molecular weight 1200) in a 1:1 mixed solvent of ethanol and water to form a b-PEI solution with a concentration of 20mM;

[0066] (2) Disperse PLGA (molecular weight 100,000) nanoparticles with particle diameters of 300 nm, 530 nm and 1000 nm into the b-PEI solution prepared in step (1) to obtain a suspension with a mass concentration of 5 mg / mL;

[0067] (3) After sonicating the suspension prepared in step (2) at 100 W for 10 min, 20 min, 30 min, 40 min, 60 min and 80 min, the suspension was centrifuged at a speed of 11000 r / min for 8 min to collect the product. The resulting product was washed 3 times with water and freeze-dried.

[0068] (4) The nanoparticles prepared in step (3) with different aminolysis treatment times are prepared into suspensions of the ...

Embodiment 2

[0079] Example 2. Preparation of hybrid hydrogel

[0080] (1) Disperse the aminolysis nanoparticles (with a particle size of 300nm) obtained in Example 1 after aminolysis for 60 minutes in deionized water, and add an equal volume of four-arm star polyethylene glycol succinate (4-arm- PEG-SC, molecular weight 10k) polymer aqueous solution, so that the solid content of the nanoparticles is 2.5%, and the mass percentage concentration of the polymer is 2.5%.

[0081] (2) Vortex the mixture obtained in step (1) to obtain a hydrogel;

[0082] (3) freeze-dry the hydrogel prepared in step (2), and analyze by SEM.

[0083] The scanning electron micrograph of the hybrid hydrogel prepared in this example is as follows Image 6 Shown.

Embodiment 3

[0084] Example 3. Preparation of hybrid hydrogel

[0085] (1) Disperse the nanoparticles (with a particle size of 300 nm) obtained by aminolysis in Example 1 for 60 minutes in deionized water, and add an equal volume of four-arm star polyethylene glycol succinate (4-arm-PEG- SC, the molecular weight is 10k) polymer aqueous solution, so that the solid content of the nanoparticles is 7.5%, and the mass percentage concentration of the polymer is 2.5%.

[0086] (2) Vortex the mixture obtained in step (1) to obtain a hydrogel;

[0087] (3) freeze-dry the hydrogel prepared in step (2), and analyze by SEM.

[0088] The scanning electron micrograph of the hybrid hydrogel prepared in this example is as follows Figure 7 Shown.

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Abstract

The invention discloses a polyester-nanoparticle-chemical-crosslinking-based hybrid hydrogel, and a preparation method and application thereof. The preparation method of the hybrid hydrogel comprises the following steps: 1) ammonolysis of nanoparticles: carrying out ammonolysis on nanoparticles by using branched polyethyleneimine, wherein the nanoparticles are hollow nanoparticles or solid nanoparticles; and 2) preparation of hybrid hydrogel: mixing the nanoparticles treated by the step 1) with a polymer to obtain a mixed solution, and carrying out vortex treatment on the mixed solution to obtain the hybrid hydrogel. By regulating the size and solid content of the nanoparticles and the concentration of the polymer, the prepared hydrogel has adjustable pore size, mechanical properties and surface amino residue level. The nanoparticles in the hydrogel are an ester-bond-containing degradable high-polymer material, the polymer is PEG (polyethylene glycol) molecules, and both the nanoparticles and polymer have the characteristics of favorable biodegradability and favorable biocompatibility.

Description

Technical field [0001] The invention relates to a hybrid hydrogel and a preparation method and application thereof, in particular to a hybrid hydrogel based on chemical cross-linking of polyester nano particles, and a preparation method and application thereof. Background technique [0002] Hydrogel has three-dimensional network structure, good biocompatibility, and adjustable mechanical properties. It can simulate natural tissues and has potential applications in the field of tissue engineering. The biological activity in the process of tissue regeneration is highly dependent on the 3D structure of the hydrogel. The interpenetrating porous structure is beneficial to the growth and migration of cells, as well as the transportation of nutrients and the discharge of waste liquid. At the same time, the porous structure can also be cells. The growth provides short-term space and support. Surface properties, such as roughness, morphology and chemical groups, have a significant effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L71/08C08L79/02C08L67/04A61K9/51A61K47/59A61L27/18A61L27/20A61L27/24A61L27/52A61K31/4409A61K31/337
CPCA61K9/5138A61K9/5153A61K31/337A61K31/4409A61L27/18A61L27/20A61L27/24A61L27/52C08J3/075C08J2371/00C08J2379/02C08L71/00C08L79/02C08L67/04
Inventor 吴德成庄亚平沈红
Owner INST OF CHEM CHINESE ACAD OF SCI
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