Method for producing pentamethylene diamine by using carbon dioxide desorption process through fermentation

Abstract

The invention belongs to the field of biological fermentation engineering, and provides a method for producing pentamethylene diamine by using a carbon dioxide desorption process through fermentation. The method comprises the following steps: culturing cells for expressing lysine decarboxylase so as to obtain a whole-cell fermentation liquid containing pentamethylene diamine; extracting pentamethylene diamine from the whole-cell fermentation liquid obtained in the step (1), wherein carbon dioxide in the whole-cell fermentation liquid is desorbed before strong base is added into the whole-cell fermentation liquid. By adopting the method, the yield of pentamethylene diamine can be greatly increased.

Description

technical field [0001] The present invention belongs to the field of biological fermentation engineering. Specifically, the present invention relates to the production of 1,5-pentanediamine by biocatalysis and its separation and extraction technology, that is, the technology of separating and extracting pentamethylenediamine in whole-cell catalytic liquid by carbon dioxide removal method . Background technique [0002] 1,5-Pentanediamine (1,5-Pentanediamine), also known as cadaverine (Cadaverine), 1,5-diaminopentane (1,5-Diaminopentane), can be polymerized into high molecular polyamide with dibasic acid material (i.e. nylon). The global production of about 7 million tons of polyamide materials every year consumes a lot of petrochemical resources. Therefore, the biosynthesis of 1,5-pentanediamine, an important constituent monomer of polyamide, has important economic and ecological significance. [0003] The whole-cell catalysis method uses lysine as a substrate, and uses ...

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Problems solved by technology

[0005] In the prior art of separation and extraction of pentamethylenediamine disclosed at present, the yield of pentamethylenediamine carboxylate obtained by crystallization is low, and impurities such as residual lysine are difficult to further refine, and the obtained pentamethylenediamine carboxylate When used as a polyamide resin film material, surface appearance defects such as fish eyes will occur and affect the fluidity of injection molding (CN101578256A)

The yield of pentamethylenediamine obtained by the extraction method is low; the residual organic solvent reduces the purity of the pentamethylenediamine product; the organic solvent has a strong odor, high toxicity, and is flammable and explosive, which increases the difficulty of practical application; the organic solvent needs to be recovered, Increased process flow and energy consumption

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