Detection method for measuring eight preservatives in reconstituted tobaccos simultaneously

A technology of reconstituted tobacco leaves and detection methods, applied in the field of physical and chemical testing, to achieve the effects of fast analysis, guaranteed extraction efficiency, and easy promotion

Inactive Publication Date: 2016-08-24
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no public reports on the detection methods for reconstituted tobacco leaves. In order to ensure the smoking saf...

Method used

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  • Detection method for measuring eight preservatives in reconstituted tobaccos simultaneously
  • Detection method for measuring eight preservatives in reconstituted tobaccos simultaneously
  • Detection method for measuring eight preservatives in reconstituted tobaccos simultaneously

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Experimental program
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Embodiment 1

[0045] Instruments, reagents and materials: American AB SCIEX 5500LC-MS / MS; American Agilent 1290HPLC; AthenaC1-WP chromatographic column (150mm×4.6mm, particle size 5μm), 0.22μm microporous filter membrane, ultrasonic cleaner; ultrapure water ( 18MΩ), methanol (chromatographically pure, Bailingwei Chemical Reagent Company).

[0046] Preparation of the extract: Prepare a solution with a volume ratio of methanol-formic acid-water of 80:0.1:19.9 as the extract.

[0047] Preparation of internal standard solution: weigh a certain amount of salicylic acid-d 4 , using methanol-formic acid-water solution to prepare an internal standard solution with a concentration of 12.5 μg / mL;

[0048] Preparation of mixed standard solution: weigh a certain amount of dehydroacetic acid, sorbic acid, benzoic acid, salicylic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate Esters, use methanol-formic acid-water solution to prepare a mixed s...

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Abstract

The invention provides a detection method for measuring eight preservatives in reconstituted tobaccos simultaneously. The method comprises the steps that a methanol-formic acid-water solution is used as extraction liquid, a reconstituted tobacco sample is subjected to ultrasonic extraction, the extracting solution is filtered by an organic filter film, a high performance liquid chromatography-tandem mass spectrometry combined method is adopted for measuring eight preservatives comprising dehydroacetic acid, sorbic acid, benzoic acid, salicylic acid, methyl 4-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate and butyl p-hydroxybenzoate. An isotope dilution internal standard method is adopted for quantification to avoid quantitative separation and purification difficulty of a complex mixture system; due to the fact that the methanol-formic acid-water solution is used as the extraction liquid, extraction efficiency is ensured, the amount of organic impurities in the sample entering a chromatographic column can be reduced, and the chromatographic column is effectively protected. The method is high in analysis speed, good in repeatability and recovery rate and applicable to analysis of large-batch products in industry.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing, and in particular relates to a method for measuring the contents of eight preservatives in reconstituted tobacco leaves. Background technique [0002] In recent years, with the development of the food industry, the food additive industry has developed rapidly. Preservatives are widely used because they can kill certain microorganisms or inhibit their growth, prevent food from mildewing and rotting, and prolong the shelf life. Dehydroacetic acid, sorbic acid, benzoic acid, salicylic acid, methyl p-hydroxybenzoate, ethyl ester, propyl ester, and butyl ester are all preservatives. Long-term excessive intake will affect the balance of human metabolism and cause certain damage to human health. damage. The "GB 2760-2014 Standards for the Use of Food Additives" has limited the maximum usage of these preservatives in food, and issued relevant testing standards for monitoring. [0...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 范忠刘鸿李小兰陈志燕周芸潘玉灵曾德芬唐桂芳韦入丹陈瑶王清波
Owner CHINA TOBACCO GUANGXI IND
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