Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of a kind of Panax notoginseng endophytic fungus Acremonium acremonium

An endophytic fungus, Acremonium acremonium technology, applied in the field of bioengineering, can solve the problems of notoginseng endophytic fungus research and related applications that have not been reported yet, and achieve the reduction of the use of pesticides and fertilizers, simple process, and single material Effect

Inactive Publication Date: 2019-08-06
杜云龙
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Panax notoginseng is a kind of traditional Chinese medicine, which has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain, but the research and related application of Sanqi endophytic fungi have not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a kind of Panax notoginseng endophytic fungus Acremonium acremonium
  • Preparation method and application of a kind of Panax notoginseng endophytic fungus Acremonium acremonium
  • Preparation method and application of a kind of Panax notoginseng endophytic fungus Acremonium acremonium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The preparation method and application of a kind of notoginseng endophytic fungus Acremonium acremonium of the present invention will be further described in detail in conjunction with specific examples below.

[0029] The preparation of a kind of Panax notoginseng endophyte acremonium acremonium of the present invention:

[0030] Cut off the dormant buds from the root of Panax notoginseng, wash the surface with clean water, then soak in 70% ethanol for 1.5 minutes, and wash 3 times with sterile water; put the dormant buds into a solution containing 10% sodium hypochlorite, and disinfect the surface for 10 minutes , and then washed 3 times with sterile water; then put the dormant buds into the Erlenmeyer flask containing MS medium, and after 1 month of light culture, the mycelium grew. Single spores were picked under a light microscope and cultured in PDA medium.

[0031] When the diameter of the colony grows to 4 cm, pick a part of mycelia and spores for morphological...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of the endophytic fungus Acremonium acremonium notoginseng and its application. Cut off the dormant buds from the root of notoginseng, wash the surface with clean water, soak it with 70% ethanol for 1.5 minutes, and use Wash 3 times with bacterial water; put the dormant buds into a solution containing 10% sodium hypochlorite, disinfect the surface for 10 minutes, and then rinse 3 times with sterile water; then put the dormant buds into the Erlenmeyer flask containing MS medium, light After culturing for 1 month, the hyphae grew out. Single spores were picked under a light microscope and cultured in PDA medium. The notoginseng endophytic fungus acremonium acremonium can promote the germination of notoginseng and the development of root system.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of the notoginseng endophytic fungus Acremonium acremonium and its application. Background technique [0002] Endophytic fungi are a special type of fungi that grow in healthy living plant tissues. They are normal flora in plant tissues, mainly in tissues below the surface cell layer, and the host tissues are temporarily asymptomatic. Endophytic fungi coexist with their hosts for a long time, so there may be a genetic system that can exchange genetic information with plant hosts, have unique biological and genetic characteristics, and can produce new compounds with various biological activities, such as antibiotics, plant Growth regulators, immunosuppressive active substances, antitumor active substances, etc. The content of active substances derived from plants is generally very low, and the plant resources in the world are decreasing day by day, so the cost o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01G7/06A01C1/00C12R1/645
CPCA01C1/00A01G7/06A01N63/30C12N1/145C12R2001/645
Inventor 杜云龙
Owner 杜云龙
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com