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A kind of preparation method of clear mung bean superoxide dismutase liposome

A technology of dismutase lipid and superoxide, which is applied in the fields of biochemical equipment and methods, oxidoreductase, liposome delivery, etc. problem, to achieve the effect of low embedding cost, high encapsulation rate and simple method

Active Publication Date: 2019-08-13
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching, the preparation method of bright mung bean superoxide dismutase liposome has not been retrieved

Method used

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  • A kind of preparation method of clear mung bean superoxide dismutase liposome
  • A kind of preparation method of clear mung bean superoxide dismutase liposome
  • A kind of preparation method of clear mung bean superoxide dismutase liposome

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Effect test

Embodiment 1

[0034] A preparation method of bright mung bean superoxide dismutase liposome is prepared by using bright mung bean and cholesterol as raw materials. The bright mung bean is provided by Anhui Yanzhifang Food Co., Ltd., and the cholesterol is food grade.

[0035] The specific operation method includes the following steps:

[0036] 1. Grinding and drying bright mung beans: crushing bright mung beans with crushing equipment, adopting vacuum drying equipment to dry under reduced pressure at a temperature of 40°C and a vacuum of 20-80kPa, and passing through a 80-mesh sieve to obtain bright mung bean powder.

[0037] 2. Extract Ming mung bean superoxide dismutase: add Ming mung bean powder to pH 7 phosphate buffer, use liquid-solid ratio 22:1 (mL / g), ultrasonic power 120w, and temperature 44°C to extract Ming mung bean Mung bean powder was extracted for 90 minutes to obtain mixture I.

[0038] 3. Hot water bath treatment: put the above mixture I in a hot water bath at 44°C for 2 h...

Embodiment 2

[0060] A preparation method of bright mung bean superoxide dismutase liposome is prepared by using bright mung bean and cholesterol as raw materials. The bright mung bean is provided by Anhui Yanzhifang Food Co., Ltd., and the cholesterol is food grade.

[0061] The specific operation method includes the following steps:

[0062] 1. Grinding and drying bright mung beans: crushing bright mung beans with crushing equipment, adopting vacuum drying equipment to dry under reduced pressure at a temperature of 50°C and a vacuum of 20-80kPa, and passing through a 100-mesh sieve to obtain bright mung bean powder.

[0063] 2. Extraction of bright mung bean superoxide dismutase: add bright mung bean powder to pH7 phosphate buffer, and use ultrasonic-assisted method to extract bright mung bean powder. After the extraction is completed, a mixture of bright mung bean powder and phosphate buffer is obtained, named: mixture I.

[0064] 3. Hot water bath treatment: The mixture I was bathed in...

Embodiment 3

[0079] A preparation method of bright mung bean superoxide dismutase liposome is prepared by using bright mung bean and cholesterol as raw materials. The bright mung bean is provided by Anhui Yanzhifang Food Co., Ltd., and the cholesterol is food grade.

[0080] The specific operation method includes the following steps:

[0081] 1. Crush and dry the bright mung bean: crush the bright mung bean with crushing equipment, dry the bright mung bean with vacuum drying equipment, and pass through a 100-mesh sieve to obtain bright mung bean powder.

[0082] 2. Extraction of bright mung bean superoxide dismutase: add bright mung bean powder to pH7 phosphate buffer, and use ultrasonic-assisted method to extract bright mung bean powder. After the extraction is completed, a mixture of bright mung bean powder and phosphate buffer is obtained, named: mixture I.

[0083] 3. Hot water bath treatment: the mixture I was bathed in hot water at 42°C for 1 hour.

[0084] 4. Centrifugation: Centr...

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Abstract

The invention provides a preparation method for a bright mung bean superoxide dismutase liposome, belonging to the technical field of superoxide dismutase. The preparation method comprises the following steps: crushing and drying bright mung bean; extraction of bright mung bean superoxide dismutase; hot bath treatment; centrifugal filtering so as to obtain supernatant I and filter residue I; fractional precipitation with ammonium sulfate; treatment with a G-200 sephadex column; extraction of the filter residue I with anhydrous petroleum ether; digestion of a mixture II in 95% ethanol; dissolving of paste I in ether; dissolving of paste II in acetone; crushing of a foamed solid; dissolving of powdery extract I in a mixed solvent; addition of cholesterol into a mixed solution I; injection of a superoxide dismutase phosphate buffer; pressure-reduced drying; dissolving in a phosphate buffered solution; etc. According to the invention, the filter residue extracted from the bright mung bean is used for preparation of the bright mung bean superoxide dismutase liposome; the method is simple; the prepared bright mung bean superoxide dismutase liposome has the characteristics of high encapsulation efficiency, stable properties, low embedding cost, wide sources of raw materials used for preparation, etc.; and the method is beneficial for comprehensive utilization of bright mung bean.

Description

technical field [0001] The invention relates to a mung bean extract and a preparation method thereof, in particular to a preparation method of a bright mung bean superoxide dismutase liposome. Background technique [0002] green beans( Vigna radiate L. ), which is the mature seed of the leguminous herb mung bean (Phaseolus radiatus). Modern pharmacological research shows that mung bean has antibacterial and antibacterial, anti-tumor, anti-oxidation, improving immunity, lowering blood fat, and detoxification effects. [0003] Mingguang Mung Bean (hereinafter referred to as "Ming Mung Bean") is mainly produced in Mingdong, Shiba, Jianxi, Guandian and other townships in Mingguang City. With its bright green color, large grains and thin skin, clear soup that is easy to rot, and delicious fragrance, the quality is the highest in the country. It is known as "bright green" and was once used as a tribute. In 2008, "Mingguang Mung Bean" was listed as a "National Geographical Indic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/44A61K36/48A61K9/127A61K47/46A61P39/06A61P35/00A61P37/04A61P39/00A61P9/10A61P17/08
CPCA61K9/127A61K36/48A61K38/446A61K47/46C12Y115/01001
Inventor 李梅青王星张瑜代蕾莉
Owner ANHUI AGRICULTURAL UNIVERSITY
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