Application of nano-sulfur hydrosol as an anti-tumor "adjuvant drug"
An anti-tumor drug, nano-sulfur technology, applied in the direction of anti-tumor drugs, sulfur compounds, drug combinations, etc., can solve the problems of unclear function and role of drugs, weaken the toxicity of drugs such as monarchs and ministers, and achieve high biocompatibility. Safety, enhanced antitumor activity, enhanced absorption effect
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Embodiment 1
[0034] Under normal pressure (1 standard atmospheric pressure), add 20mL of PEG-200 to two 100mL Erlenmeyer flasks, stir and heat to 200°C, then add 20mg of sublimated sulfur solid powder to the two volumetric flasks , Keep heating and stirring to make it fully mixed until the dark yellow color does not change. Then add 60mL and 20mL ultrapure water at room temperature (effective temperature range: 18-28°C, optimum temperature: 25°C) to obtain sulfur concentrations of 0.25mg·mL -1 and 0.50mg·mL -1 , PEG are 25% and 50% target products respectively, and the average particle size of the nanoparticles in the products is in the range of 640-950nm, as shown in Table 1. The product can be stored in the form of a sol in an aqueous solution at 2-20° C., that is, nano-sulfur hydrosol (Nano-S).
[0035] The particle size range of the nanometer sulfur particles of table 1 embodiment 1
[0036]
[0037] Note: The main particle size refers to the particle size of the largest number o...
Embodiment 2
[0040] Using the nano-sulfur hydrosol a prepared in Example 1, and copper sulfate with different mass concentrations at normal pressure, after reacting for 2 hours at 37°C, centrifuge at 20000 × g to get the supernatant, and titrate the amount of copper in the supernatant, And calculate the amount of copper captured by nano-sulfur. The result is as figure 2 As shown, the nano-sulfur hydrosol a prepared in Example 1 can reach 2.84mg·mg to the capture amount of copper -1. It shows that our nano-sulfur hydrosol has a good adsorption capacity for copper.
[0041] With nano-sulfur hydrosol a prepared in Example 1, A375 malignant melanoma cells and MCF-7 breast cancer cells were treated with different doses (cell concentration range: 3~10×10 4 cell mL -1 , optimal cell concentration: 8×10 4 cell mL -1 ). After 24 hours, the viability of the cells was detected, and the half inhibitory concentration (IC50) value was calculated. The result is as image 3 As shown, the nano-su...
Embodiment 3
[0043] With the nano-sulfur hydrosol a prepared in Example 1, in the form of 2 μg·mL -1 (Effective mass concentration range: 0.5 μg mL -1 ~3μg·mL -1 , preferred mass concentration: 2μg·mL -1 ) doses to treat A375 malignant melanoma cells and MCF-7 breast cancer cells respectively (cell concentration range: 3~10×10 4 cell mL -1 , optimal cell concentration: 8×10 4 cell mL -1 ), 12 hours later, the culture medium was centrifuged at 20000×g to remove nano-sulfur, the supernatant was added to the cell culture system, and different doses of cisplatin were added to continue the treatment for 24 hours, and then the content of cisplatin in the cells and the survival rate of the cells were detected. The result is as Figure 4 , 5 As shown, the short-term treatment of low-dose nano-sulfur can significantly increase the absorption of cisplatin in A375 malignant melanoma cells and MCF-7 breast cancer cells, and can also significantly enhance the anti-tumor activity of cisplatin. T...
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