Method for extracting alkanin from arnebia roots

Abstract

The invention discloses a method for extracting alkanin from arnebia roots, and belongs to the technical field of alkanin preparation. The method comprises the following steps: firstly burying fresh arnebia roots into soil, irrigating the arnebia roots with a methane tank supernate which is rich in microorganism and presents faintly acid, then slicing the arnebia roots, obtaining arnebia root pulp after boiling and grinding, adding pectinase and cellulose, performing isothermal stirring and then performing enzyme deactivation treatment, adding ethyl alcohol and urea after the enzyme deactivation treatment, filtering to obtain filter liquor, heating the filter liquor to be boiled, adding dry ice to the filter liquor, removing the filter liquor through extraction filtration, washing filter cakes with deionized water, and drying the filter cakes, so as to obtain alkanin. The method is simple and convenient to operate, expensive equipment is not required, toxic organic solvent does not exist during the whole preparation process, the yield of the obtained alkanin is high while the cost is saved, by-products are less, and the method can be applied to industrial mass production.

Description

technical field

[0001] The invention discloses a method for extracting shikonin from shikonin roots, belonging to the technical field of shikonin preparation. Background technique

[0002] Comfrey, aliases: hard comfrey, soft comfrey. It is a herbal medicine included in the Chinese Pharmacopoeia, and its medicinal source is the dried root of Xinjiang Comfrey, Comfrey or Inner Mongolia Comfrey. The most important compounds in comfrey root are shikonaquinone compounds, mainly including shikonin, acetylshikonin, β,β-dimethylacryloylshikonin, isovalerylshikonin, isobutyrylshikonin Among them, the content of β,β-dimethylacryloylshikonin is the highest, followed by acetylshikonin, and the content of shikonin is the lowest. Shikonadhiquinone compounds have been identified by the National Cancer Institute of the United States as substances with anti-tumor activity. They have a wide range of biological activities including: antibacterial, anti-AIDS, promotion of burn wound healing,...