Great burdock fruit extract and preparation method thereof and application of great burdock fruit extract in bacteriostasis

Abstract

The invention discloses a great burdock fruit extract. A preparation method of the great burdock fruit extract comprises the following steps that great burdock fruit is taken and smashed; a 65%-80% ethanol solution is added according to the solid-liquid ratio of 1:(6-10), extracting is conducted in a water bath at the temperature of 60 DEG C+ / -5 DEG C for 1.5 h-2.5 h, suction filtering is conducted, filter residues are extracted for 1.5 h-2.5 h under the same conditions, and suction filtering is conducted; filtrates obtained through two times of filtering are mixed, a mixture is evaporated and concentrated, absolute ethanol is added, standing by sealing is conducted for 18 h-24 h, suction filtering is conducted again, freeze drying is conducted to obtain powder, that is, the great burdock fruit extract. Experiments prove that the great burdock fruit extract has the significant inhibition effect on various food-borne pathogenic bacteria such as escherichia coli O157:H7, pseudomonas aeruginosa, listeria monocytogenes, yersinia, salmonella enteritidis, staphylococcus aureus and vibrio parahemolyticus and can be used for preparing a bacteriostatic preparation.

Description

technical field [0001] The invention relates to burdock fruit extract, its preparation method and its application in antibacterial. Background technique [0002] Burdock is a rare plant that grows naturally in the wild. Few researchers have paid attention to it before. Therefore, almost no one has studied its components, and no one knows its value, and its development and utilization are even less There are fewer of them. However, with the development of modern science, people's research on burdock is constantly deepening, and its nutritional and pharmacological values ​​are gradually being understood by people, and burdock has gradually been planted on a large scale. The planting area has reached more than 23,300 hectares. [0003] Burdock seed is the dried seed of burdock after it matures. It was first recorded in "Bencao Tujing". The production of burdock in the three northeastern provinces is very large, and it is called "Guanlizi" by people. It is sold all over the c...

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[0018] The research and analysis of arctigenin on the production of tumor necrosis factors, as well as the research and analysis on the regulation of lymphatic proliferation and immunity, found that arctigenin can play a certain role in the release of NO caused by interferon-γ signal. degree of inhibition, but a very large increase in the production of NO induced by lipopolysaccharide

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