A kind of sericin-alginate composite hydrogel and preparation method thereof

A technology of composite hydrogel and sericin, which is applied in the field of sericin-alginate composite hydrogel and its preparation, can solve the problem of increasing material production cost and workload, lack of cell adhesion performance and controllable Degradability and other issues, to achieve good drug release ability, reduce surgical trauma, good biocompatibility effect

Active Publication Date: 2020-11-10
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alginic acid has good biocompatibility, is non-toxic, and is easy to combine with divalent cations to form a gel. There is no need to use any harmful cross-linking agents in the gel process. It has been widely used in the field of biomedicine. Alginic acid Salt gel is often used in the fields of cell culture, tissue transplantation and drug release, but due to its lack of cell adhesion and controllable degradation, it is often necessary to further modify alginic acid (salt) (such as RGD modification to improve Adhesion of the material to cells) can meet the actual needs, which greatly increases the production cost and workload of the material

Method used

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  • A kind of sericin-alginate composite hydrogel and preparation method thereof
  • A kind of sericin-alginate composite hydrogel and preparation method thereof
  • A kind of sericin-alginate composite hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Preparation of sericin-alginate composite hydrogel provided by the present invention

[0060] 1. Selection of silkworm cocoons:

[0061] Silkworm cocoons of silkworm silk fibroin-deficient mutant varieties were selected (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the silkworm silk fibroin-deficient mutant varieties were preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, preservation number (or commodity number) 185Nd -s, 140Nd-s, 139Nd-s) as raw materials, the main chemical composition is: sericin.

[0062] 2. Extraction and separation of sericin

[0063] 1) Weigh 1 g of silkworm cocoons (purchased from the Institute of Sericulture, Chinese Academy of Agricultural Sciences) and cut them into 1 cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 350...

preparation example 1

[0078] Sericin aqueous solution and CaCl 2 The aqueous solution ionic cross-linking agent is mixed to form mixed liquid A, and the alginate aqueous solution and glutaraldehyde covalent cross-linking agent are mixed to form mixed liquid B, and then mixed liquid A and mixed liquid B are mixed (for example, with mixed liquid The two syringes of A and mixed solution B were connected by Luer and pushed each other dozens of times to mix well, and then stood at 37°C for 5 minutes to form sericin-alginate composite hydrogel.

preparation example 2

[0080] Sericin aqueous solution and Ca 2 SO 4 Mix the ionic cross-linking agent to form a mixed solution A, mix the alginate aqueous solution and the glutaraldehyde covalent cross-linking agent to form a mixed solution B, then mix the mixed solution A and the mixed solution B in equal volumes, mix well and place at 37 °C for 0.5 hour to obtain sericin-alginate composite hydrogel.

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Abstract

The present invention provides a sericin-alginate composite hydrogel and a preparation method thereof, the method comprising: 1) weighing the cocoons of silkworm silk fibroin-deficient mutant varieties, extracting with LiBr or LiCl aqueous solution, and then dialysis and purification, Concentrate to obtain a non-degradable sericin aqueous solution with a mass percentage concentration of 1.5-10%; 2) prepare an alginate aqueous solution with a mass percentage concentration of 0.1-10%; 3) make the above-mentioned The sericin aqueous solution and the alginate aqueous solution are mixed at a ratio of 100-0.01:1 to form a sericin-alginate composite hydrogel, and the cross-linking agent includes an ionic cross-linking agent and / or a covalent cross-linking agent. The scheme of the present invention can obtain sericin-alginate composite hydrogel with interpenetrating network, and the obtained composite hydrogel not only has excellent fluorescence characteristics, deformation memory and elastic modulus controllability, but also has good Biocompatibility, cell adhesion, can support cell survival, proliferation and migration for a long time.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a sericin-alginate composite hydrogel and a preparation method thereof. Background technique [0002] Silk Sericin (Silk Sericin) is a natural macromolecular viscous protein wrapped on the surface of silk fibers, accounting for about 20-30% of the cocoon content, composed of polypeptides with a molecular weight of 24-400kDa, and its molecules are composed of serine, asparagus It is composed of 18 kinds of amino acids such as amino acid and glycine. For a long time, due to the lack of understanding of sericin and the limitations of research, sericin has been treated as waste in the silk reeling industry, wasting a lot of valuable natural resources. In recent years, it has been found that sericin has biological properties such as moisturizing, antibacterial, anti-oxidation, anti-coagulation, and promoting cell adhesion and proliferation. At the same time, sericin is h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/40C08J9/28C08J3/075C08J3/24C08L89/00C08L5/04C08K5/07C08K3/16C08K3/26C08K3/30C08K5/1545
Inventor 王琳王征张业顺刘佳黄雷乞超徐鲁明宋煜杨文谢洪建王健张诚
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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