Ganoderan lipid-based cubic liquid crystal as well as preparation method and application thereof

A Ganoderma lucidum polysaccharide and lipid technology, which is applied in the field of Chinese veterinary medicine, can solve the problems of Ganoderma lucidum polysaccharide instability, low bioavailability, and rapid metabolism in the body, and achieve the effects of increasing antibody potency, prolonging drug efficacy, and simple operation

Active Publication Date: 2016-10-12
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a kind of ganoderma polysaccharide lipid cubic liquid crystal, to solve the problems in the prior art that the ganoderma polysaccharide is unstable, prone to aggregation, fast metabolism in vivo, low bioavailability and large dosage.

Method used

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  • Ganoderan lipid-based cubic liquid crystal as well as preparation method and application thereof
  • Ganoderan lipid-based cubic liquid crystal as well as preparation method and application thereof
  • Ganoderan lipid-based cubic liquid crystal as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation of Ganoderma lucidum polysaccharide lipid cubic liquid crystal

[0053] (1) Screening of methods

[0054] Method 1 "Buk" phase spontaneous emulsification method:

[0055] Weigh 0.1g of phytantriol, 0.01g of F127, and 0.075g of propylene glycol into a 20ml vial, add 10ml of chloroform, and ultrasonically make it fully dissolved. Place it in a 50mL rotary steaming flask and evaporate under reduced pressure at 45°C to remove the organic solvent. When the solution in the round bottom flask does not decrease significantly, introduce 10uL of an aqueous solution containing 10mg of Ganoderma lucidum polysaccharide, mix it thoroughly with ultrasound, and then disperse it to 5mL Stir in double-distilled water for 5-10 minutes to form the Ganoderma lucidum polysaccharide lipid cubic liquid crystal suspension and store at 4°C.

[0056] Method 2 Precursor-injection method:

[0057] Weigh 0.1g of the lipid material phytantriol and 0.075g of propylene glycol, add 1.5ml o...

Embodiment 2

[0074] From the scanning electron microscope picture, it can be seen that the Ganoderma lucidum polysaccharide cubic liquid crystal nanoparticles are well dispersed in water, and the difference in particle size is small, and the appearance is cubic. From the particle size analysis, it can be seen that the cubic liquid crystal encapsulates the Ganoderma lucidum polysaccharide in particle size. Increase( Figure 2A ), while SAXS displays the ratio of the scattering vector corresponding to each scattering peak as ( Figure 2B ), indicating that it is a Pn3m crystal structure, and the surface potentials of Cub and Cub-PS are -14.33±0.75 and -16.13±0.42, respectively. Electron microscopy, particle size and potential analysis show that the Ganoderma lucidum polysaccharide lipid cubic liquid crystal has excellent physical and chemical properties. Example 2 Comparison of Ganoderma lucidum polysaccharide lipid cubic liquid crystal immune enhancement activity

[0075] Taking the Ganoderma...

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Abstract

The invention discloses a preparation method of a ganoderan lipid-based cubic liquid crystal. The liquid crystal is prepared by using double distilled water as a dispersion medium with a precursor-injection method. The invention further discloses an application of the prepared ganoderan lipid-based cubic liquid crystal to preparation of drugs for enhancing body immunity. Compared with the prior art, the method has the advantages that the encapsulation efficiency is high, the prepared product is relatively good in stability and high in immune enhancement activity, and the immune enhancement activity of ganoderan is significantly enhanced.

Description

Technical field [0001] The invention belongs to the field of Chinese veterinary medicine, and specifically relates to a ganoderma polysaccharide lipid cubic liquid crystal and a preparation method and application thereof. Background technique [0002] Animal diseases are mainly prevented by vaccines, but many vaccines have disadvantages such as weak immunogenicity and require the assistance of immune enhancers to generate a strong immune response. Existing commonly used immune enhancers such as oil adjuvants, aluminum glue adjuvants, etc. often have the shortcomings of strong irritation and residues. Due to the green and safe characteristics of traditional Chinese medicine, the development of immune enhancers from traditional Chinese medicine has become a hot spot. [0003] Ganoderma lucidum polysaccharide is the main component of Ganoderma lucidum that has immune-enhancing activity. Ganoderma lucidum polysaccharide not only provides glycogen energy and constitutes the structure o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10A61K31/715A61K47/34A61P37/04A61P31/20
CPCA61K9/10A61K31/715A61K36/074A61K39/12A61K47/10A61K2039/5252A61K2039/545A61K2039/552C12N2750/10034A61K2300/00
Inventor 王德云刘振广伯若楠胡元亮刘家国武毅
Owner NANJING AGRICULTURAL UNIVERSITY
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