A preparation method of doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles
A technology of hyaluronic acid modification and polyethyleneimine, which is applied in the field of preparation of drug-loaded nanomaterials, to increase the circulation time in the body, sensitive CT imaging effect, and realize the effect of targeted therapy
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Embodiment 1
[0083] (1) In 50 mL PLA-PEG-COOH mixed solution of acetone and water (6 mg / mL), 10 mL LAP aqueous solution (10 mg / mL) was slowly added dropwise, and the reaction was stirred for 1 hour. After the reaction, all the obtained solution was transferred to a dialysis bag with a cutoff analysis volume of 8000-14000. Put the dialysis bag into a beaker filled with 2L deionized water for dialysis. The dialysis lasts for 2-4 days, and the water is changed 3-4 times a day. After the dialysis, the product in the dialysis bag was transferred to a 100mL glass bottle and stored at 4°C to obtain polyethylene glycol-modified LAP-PLA-PEG-COOH.
[0084] (2) Take the above 30mL LAP-PLA-PEG-COOH aqueous solution (3mg / mL), add 2mL EDC aqueous solution (8.42mg / mL), stir and react for 3 hours. Then it was added dropwise to 10.98 mL of PEI in water (1 mg / mL), and reacted with rapid stirring for 3 days. After the reaction, all the obtained solution was transferred to a dialysis bag with a cut-off anal...
Embodiment 2
[0087] Analyze the stability of the material with the ultraviolet-visible absorption spectrum, take 0.5 mg / mL of the functionalized dendrimer material prepared in Example 1, the solvent is distilled water, and place it under the temperature conditions of 4°C, 37°C, and 50°C Under the conditions of pH=5, 6, 7, 8, measure its ultraviolet-visible absorption spectrum.
Embodiment 3
[0089] Get the product gained in Example 1 and use distilled water as a solvent to prepare a mother liquor with a gold concentration of 0.04M. Afterwards, 0.02, 0.01, 0.005, 0.0025 and 0.005M samples were serially diluted. At the same time, using iohexol for clinical use as a reference material, samples with corresponding iodine concentrations were diluted with distilled water. Afterwards, CT imaging tests were performed on the two sets of materials.
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