A preparation method of doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles

A technology of hyaluronic acid modification and polyethyleneimine, which is applied in the field of preparation of drug-loaded nanomaterials, to increase the circulation time in the body, sensitive CT imaging effect, and realize the effect of targeted therapy

Inactive Publication Date: 2019-01-11
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to relevant literature or patents at home and abroad, the synthesis of doxorubicin (DOX)-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles for tumor targeting therapy and CT imaging has not been reported yet.

Method used

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  • A preparation method of doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles
  • A preparation method of doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles
  • A preparation method of doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles

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Effect test

Embodiment 1

[0083] (1) In 50 mL PLA-PEG-COOH mixed solution of acetone and water (6 mg / mL), 10 mL LAP aqueous solution (10 mg / mL) was slowly added dropwise, and the reaction was stirred for 1 hour. After the reaction, all the obtained solution was transferred to a dialysis bag with a cutoff analysis volume of 8000-14000. Put the dialysis bag into a beaker filled with 2L deionized water for dialysis. The dialysis lasts for 2-4 days, and the water is changed 3-4 times a day. After the dialysis, the product in the dialysis bag was transferred to a 100mL glass bottle and stored at 4°C to obtain polyethylene glycol-modified LAP-PLA-PEG-COOH.

[0084] (2) Take the above 30mL LAP-PLA-PEG-COOH aqueous solution (3mg / mL), add 2mL EDC aqueous solution (8.42mg / mL), stir and react for 3 hours. Then it was added dropwise to 10.98 mL of PEI in water (1 mg / mL), and reacted with rapid stirring for 3 days. After the reaction, all the obtained solution was transferred to a dialysis bag with a cut-off anal...

Embodiment 2

[0087] Analyze the stability of the material with the ultraviolet-visible absorption spectrum, take 0.5 mg / mL of the functionalized dendrimer material prepared in Example 1, the solvent is distilled water, and place it under the temperature conditions of 4°C, 37°C, and 50°C Under the conditions of pH=5, 6, 7, 8, measure its ultraviolet-visible absorption spectrum.

Embodiment 3

[0089] Get the product gained in Example 1 and use distilled water as a solvent to prepare a mother liquor with a gold concentration of 0.04M. Afterwards, 0.02, 0.01, 0.005, 0.0025 and 0.005M samples were serially diluted. At the same time, using iohexol for clinical use as a reference material, samples with corresponding iodine concentrations were diluted with distilled water. Afterwards, CT imaging tests were performed on the two sets of materials.

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Abstract

The invention relates to a preparation method for adriamycin-loaded polyethyleneimine-hyaluronic acid-modified hectorite-coated gold nanoparticles. The preparation method comprises the steps that a PLA-PEG-COOH solution is dropwise added into an LAP solution, stirring and activating are conducted, the mixture is dropwise added into a PEI solution to obtain LAP-PLA-PEG-PEI, chloroauric acid and NaBH4 are added, and LAP-PLA-PEG-PEI-(Au<0>)50 is obtained through a reaction; an activated HA solution is dropwise added into a LAP-PLA-PEG-PEI-(Au<0>)50 aqueous solution, stirring and reacting are conducted, DOX is added, reacting under stirring is conducted, and then the adriamycin-loaded polyethyleneimine-hyaluronic acid-modified hectorite-coated gold nanoparticles are obtained. The preparation method is low in cost, simple, mild in condition and easy to operate and has good application prospects. The nanomaterial-loaded DOX has a good drug slow release effect by serving as a nano-drug slow release system and has the significant targeting property and obvious inhibiting effect on tumor cells expressed by a CD44 receptor.

Description

technical field [0001] The invention belongs to the field of preparation of drug-loaded nanometer materials, in particular to a method for preparing doxorubicin-loaded polyethylenimine-hyaluronic acid-modified hectorite-coated gold nanoparticles. Background technique [0002] Cancer is considered to be one of the major problems facing mankind in the 21st century. In the process of using chemical drugs to treat tumors, anticancer drugs have problems such as poor water solubility, short retention time at the lesion site, and inability to specifically recognize tumor cells, which have strong toxic and side effects on the human body, and cannot effectively achieve the killing effect of drugs on tumors. . Therefore, the development of a carrier material with tumor-targeting effect to realize drug loading, controlled release and targeted therapy is a research hotspot in nanomedicine at present. [0003] Clay-based nanoparticles, such as Laponite (LAP), not only have good biocomp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K47/02A61K31/704A61K49/04A61P35/00
CPCA61K31/704A61K47/02A61K47/36A61K49/04
Inventor 郭睿庄英史向阳
Owner DONGHUA UNIV
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