Bactericide composition containing phenylamide and benzamide bactericides and biocontrol bacterium, preparation and applications

Abstract

The invention discloses a bactericide composition containing phenylamide and benzamide bactericides and a biocontrol bacterium, a preparation and applications and belongs to the field of plant protection and pesticide science. The bactericide composition comprises phenylamide bactericides, benzamide bactericides and biocontrol bacterium powder, wherein the mass ratio of the phenylamide bactericides to the benzamide bactericides to the biocontrol bacterium powder is (1-10) to (1-10) to (1-10); the viable count of the bacterium powder is not lower than 10<6>cfu / g; the biocontrol bacterium is preferably bacillus amyloliquefaciens ZY-9-13, with collection number of CGMCC No.6162. The bactericide composition has the beneficial effects that the phenylamide bactericides can inhibit the activity of RNA polymerase in pathogenic bacteria; fluopicolide as the benzamide bactericide achieves the bactericidal effects via spectrin acting on cell membranes; zoxamide gives play to the bactericidal activity by inhibiting assembly of a mitochondrion beta-tubulin of pathogenic bacteria; by using the two types of bactericides with bacillus amyloliquefaciens cooperatively, the bactericide composition has excellent control effects on tobacco root knot nematode and tobacco black shank.

Description

technical field [0001] The invention relates to a fungicide composition containing phenylamides, benzamide fungicides and biocontrol bacteria, as well as a preparation and application of the fungicide composition, belonging to the fields of plant protection and pesticide science. Background technique [0002] Tobacco black shank (Tobacco black shank) is a worldwide devastating soil-borne disease, and its pathogen is Phytophthora parasitica var nicotianae (Breda de Hann) Tuker]. Phytophthora nicotianae can infect tobacco plants from the seedling stage to the adult plant stage, mainly infecting tobacco stems, and can also infect leaves. Tobacco black shank forms water-soaked black spots at the base of the stem, the cortical tissue becomes black and depressed, and spreads up and down along the stem, and the pith is dark brown and necrotic and shrinks into a disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, et al. .Tobacco black shank early prevention technology[J].Plant Doctor...

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Problems solved by technology

For example, the combination of yellow blue-shaped bacteria and manganese zinc can control wheat scab (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. Fermentation conditions of biocontrol strain MT-06 and the control effect of compound fungicides on wheat scab[ J]. Zhejiang Agricultural Sciences, 2013 (07): 821-824), the combination of Bacillus subtilis and tebuconazole can prevent and treat broad bean wilt (Chen Zhiyi, Ren Haiying, Liu Yongfeng, etc. Tebuconazole and Bacillus subtilis synergistically control broad bean Fusarium wilt and its synergistic mechanism [J]. Journal of Pesticide Science, 2002 (4): 40-44), Bacillus subtilis, difenoconazole, azoxystrobin, etc. can control apple ring spot (Zhou Han .Coordinated control of postharvest ring blight by fungicides and biocontrol bacteria and its mechanism[D].Anhui Agricultural University, 2009), Jinggangmycin and Bacillus subtilis synergistically control rice sheath blight (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jinggangmycin and biocontrol bacteria Bs-916 synergistic disease control and synergistic mechanism[J]. Plant Protection Journal, 2003, 30(4):429-434.), etc., but the control target of the combination of the above bacteria and drugs Phytophthora nicotae

The invention patent with the publication number CN104381303A discloses a fungal drug composition for preventing and treating fungal diseases, which is composed of riboflavin and Bacillus subtilis Tpb55 bacterial powder according to the mass ratio of 1.21~2.34:97.96~98.79. Applying Bacillus subtilis Tpb55 bacterial powder, the bacterial drug composition can significantly improve the control effect of tobacco black shank, red spot disease and other diseases, and enhance the disease resistance of plants, but it can only achieve the same control effect as chemical agents

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