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Protein TRIM27 resistant to mycobacterium tuberculosis infection

A mycobacterial, anti-tuberculosis technology, applied in the field of cell biology, can solve problems such as unclear

Active Publication Date: 2016-10-26
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of TRIM27 protein in the process of mycobacterial infection of the host is still unclear

Method used

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  • Protein TRIM27 resistant to mycobacterium tuberculosis infection
  • Protein TRIM27 resistant to mycobacterium tuberculosis infection
  • Protein TRIM27 resistant to mycobacterium tuberculosis infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 TRIM27 protein against mycobacterial infection

[0016] (A) Experimental method: Infect wild-type and TRIM27-knockout macrophage U937 with Mycobacterium smegmatis (M.smegmatis) and Bacillus Calmette-Guerin (BCG), respectively. CFU changes to prove that TRIM27 plays a role in the process of resisting pathogenic bacteria infection.

[0017] (B) Results: The number of CFU of macrophage U937 cells infected by Mycobacterium smegmatis M.smegmatis was the highest after 2 hours, and the mycobacteria were gradually cleared as the infection time prolonged ( figure 1 B). After BCG was infected with U937, the number of CFU gradually decreased within 8 hours, and the number of CFU began to increase after 24 hours. The reason is that BCG contains many effector proteins that are the same as those of Mycobacterium tuberculosis, which began to interfere with the host's natural immune response and promote BCG. Intrasurvival ( figure 1 A). Comparing the CFU difference betwee...

Embodiment 2

[0018] Example 2 TRIM27 promotes the secretion of inflammatory cytokines

[0019] (A) Experimental method: use M.smegmatis ( figure 2 C,D) and BCG ( figure 2 A, B) Macrophages U937 with wild type and TRIM27 knockout were infected respectively, and the gene transcription levels of cytokines il8 and il1b at different infection time points were detected by fluorescent real-time quantitative PCR.

[0020] (B) Results: Comparing the gene transcription levels of il8 and il1b in wild-type and TRIM27-knockout U937 cells at different infection time points, the results showed that during the infection of M. and il1b gene transcription levels were significantly reduced. Therefore, during mycobacterial infection of macrophages, TRIM27 plays an important role in promoting the secretion of inflammatory cytokines to resist pathogenic infection.

Embodiment 3

[0021] Example 3 TRIM27 Promotes the Apoptotic Process of Pathogenic Bacteria Infected Cells

[0022] (A) Experimental method: M. smegmatis was used to infect wild-type and TRIM27 knockout macrophages respectively, and Western blotting was used to detect the changes in the degree of cleavage and activation of the apoptosis marker protein Caspase 3 (Cleaved-caspase 3) at different time points of infection.

[0023] (B) Results: Comparing the protein levels of Cleaved-caspase 3 in wild-type U937 and TRIM27-knockout U937 cells at different infection time points, the results showed that the level of Cleaved-caspase 3 in TRIM27-knockout U937 cells was lower, that is, the effect of TRIM27 on Promotes apoptosis ( image 3 ).

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Abstract

The invention discloses a protein TRIM27 resistant to mycobacterium tuberculosis infection and belongs to the field of cell biology. The protein TRIM27 resistant to mycobacterium tuberculosis infection can be used for developing a drug for treating phthisis or a JNK / p38 signal pathway activating agent or an NF-KappaB signal pathway inhibitor, a new thought and a molecular target are provided for research of novel anti-tuberculosis drugs, and especially the protein TRIM27 has a wide prospect on the development aspects of a variety of drugs resistant to tuberculosis and the like.

Description

technical field [0001] The invention relates to a protein TRIM27 for resisting mycobacterium tuberculosis infection, which belongs to the field of cell biology. Background technique [0002] In the 1990s, due to the emergence and global spread of multidrug-resistant tuberculosis (mul-tidrug-resistant tuberculosis, MDR-TB) and extensively drug-resistant tuberculosis (extensively drug-resistant tuberculosis, XDR TB), the global tuberculosis epidemic emerged. Pick up momentum. At present, the diagnosis and cure rate of MDR-TB is low, and a considerable number of MDR-TB patients are highly infectious. Therefore, the global MDR-TB prevention and control work is facing severe challenges. The tuberculosis pathogen Mycobacterium tuberculosis (M.tuberculosis, Mtb) is a typical intracellular bacterium, which has evolved efficient and complex methods of evading or interfering with host immune signaling pathways. Molecular mechanisms can provide new targets for the development of anti...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P31/06A61P11/00G01N33/68
Inventor 刘翠华汪静
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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