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Free radical degradation product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

A technology of degradation product, hollyhocks, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of less active research, improve immune activity, improve economic value, and achieve sustainable applied effect

Active Publication Date: 2018-06-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies on polysaccharides from stems and leaves of hollyhock mainly focus on the analysis of physical and chemical properties and monosaccharides, and there are few studies on its activity.

Method used

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  • Free radical degradation product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Free radical degradation product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Free radical degradation product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the free radical degradation product of the hollyhock stem and leaf polysaccharide of the present invention comprises the following steps:

[0032] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock

[0033] Add 100 g of hollyhock stem and leaf coarse powder, add 30 times the amount of water, and extract under reflux in a water bath at 100°C for 3 times, each time for 1 hour. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.

[0034] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock

[0035] Weigh 3 g of crude polysaccharide, ...

Embodiment 2

[0038] Example 2 Determination of Total Sugar and Uronic Acid Content of Free Radical Degradation Products of Hollyhock Stem and Leaf Polysaccharides

[0039] 1. Experimental method: with D-anhydrous glucose as the standard, the total sugar content of polysaccharides in the stems and leaves of hollyhock before and after sulfation modification was determined by the phenol-sulfuric acid method; Aldehydic acid is the standard.

[0040] 2. Experimental results: the total sugar content of hollyhock stem and leaf polysaccharides prepared in Example 1 was 99.76%, and the total sugar content of the free radical degradation products of hollyhock hollyhock stem and leaf polysaccharides decreased to 95.53%; Contains uronic acid, and does not contain uronic acid after degradation, which is consistent with the result of monosaccharide composition.

Embodiment 3

[0041] Example 3 Monosaccharide composition analysis of polysaccharides from stems and leaves of hollyhock and its free radical degradation products

[0042] 1. The experimental method uses polysaccharide samples hydrolyzed with trifluoroacetic acid, using mixed monosaccharides as standard products, PMP-derived polysaccharide samples and mixed standards, using high performance liquid chromatography to determine the composition of monosaccharides according to the peak time, and drawing each single according to the peak area. The sugar standard curve was used to determine the monosaccharide content of the samples.

[0043] 2. Experimental results: analysis of monosaccharide composition

[0044] The molar ratio of the monosaccharide composition of the stem and leaf polysaccharide of hollyhock prepared in Example 1 and the degradation product is shown in Table 1. The polysaccharide of the stem and leaf of hollyhock is composed of mannose, glucose, galactose and arabinose, wherein ...

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Abstract

The invention discloses a free radical degradation product of abelmoschus manilhot stem leaf polysaccharide and a preparation method thereof. The free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide is composed of mannose, glucose, galactose and arabinose in molar ratio of 0.34:18.56:1.0:0.36 and has the molecular weight of 552.36kDa. The preparation method comprises the following steps: optimizing an extraction separation technology by virtue of a large number of experiments, carrying out water extraction and alcohol precipitation for obtaining crude polysaccharide, then carrying out deproteinization, then purifying by adopting DEAE-52 celluosic resin, so as to obtain high-purity abelmoschus manilhot stem leaf polysaccharide, then degrading by adopting an H2O2-Vc system, and purifying, so that the free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide is obtained. The preparation method disclosed by the invention has the advantages that abandoned abelmoschus manilhot stem leaf resource is fully utilized, waste materials are changed into things of value, the free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence is obtained, and sustainable application of traditional Chinese medicine resources can be realized, so that the preparation method has good economic value and great significance for ecological environmental protection.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to a free radical degradation product of hollyhock stem and leaf polysaccharide which can improve immune activity and a preparation method thereof. Background technique [0002] Traditional Chinese medicine resources are a solid foundation for safeguarding national health and developing ethnic medicine. In recent years, the production area of ​​traditional Chinese medicine and natural medicinal biological resources has exceeded 2.40×10 6 hm 2 , the output of medicinal materials can reach 5.40×10 6 t, while the biomass of discarded plant roots and aboveground stems and leaves is as high as 1.1×10 7 ~1.6×10 7 t is 2 to 3 times the output of medicinal materials, causing serious waste of resources and environmental pollution. Therefore, in the process of industrialization of traditional Chinese medicine resources, improving the utilization value of medicinal biological resources, especiall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A61K36/185
CPCA61K31/715A61K36/185C08B37/0003C08B37/006
Inventor 江曙潘欣欣段金廒钱大玮朱悦严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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