A kind of antibacterial peptide nisin and its extraction method and application
An extraction method and antimicrobial peptide technology, which is applied in the field of deep processing of natural products, can solve the problems of low Nisin recovery rate and low activity of recovered products, and achieve good antibacterial effect, low toxicity and pollution, and growth inhibition effect
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Embodiment 1
[0030] Prepare Nisin extract, such as figure 1 As shown, it mainly includes the following steps:
[0031] (1) Preparation of Lactococcus lactis fermentation broth: prepare the Lactococcus lactis culture medium according to Table 1 and adjust the pH to 6.8. If it is a solid medium, add 1.5% agar and sterilize at 115°C for 15 minutes; Dissolve ATCC 11454 dry powder in 0.5mL liquid medium, then insert it into 5mL liquid medium at a ratio of 5% (v / v), and incubate in the incubator for 24 hours; take a little activated bacterial liquid with an inoculation needle, and streak Inoculate on a plate, and culture it statically in an incubator for 18 hours; pick a single colony from the plate, inoculate it in 30 mL of liquid medium, and cultivate it in an incubator for 19 hours. The cultivated seed solution was inoculated into fresh 200 mL liquid medium at a ratio of 3% (v / v), and cultured statically in an incubator for 16 hours, and the temperature of the incubator was 29° C.
[0032] ...
Embodiment 2
[0038] Prepare Nisin extract, such as figure 1 As shown, it mainly includes the following steps:
[0039] (1) Preparation of Lactococcus lactis fermentation broth: Prepare Lactococcus lactis culture medium according to Table 1 and adjust the pH to 6.8. If it is a solid medium, add 1.5% agar and sterilize at 115°C for 15 minutes; Dissolve ATCC 11454 dry powder in 0.5mL liquid medium, then insert it into 5mL liquid medium at a ratio of 5% (v / v), and cultivate it in a 3 incubator for 22 hours; take a little activated bacterial liquid with an inoculation needle, and mark The line was inoculated on the plate, and cultured in the incubator for 19 hours; a single colony was picked from the plate, inoculated in 30 mL liquid medium, and cultivated in the incubator for 20 hours. The cultivated seed solution was inoculated into fresh 200 mL liquid medium at a ratio of 3% (v / v), and cultured statically in an incubator for 17 hours, and the temperature of the incubator was 30° C.
[0040...
Embodiment 3
[0044] Prepare Nisin extract, such as figure 1 As shown, it mainly includes the following steps:
[0045] (1) Preparation of Lactococcus lactis fermentation broth: Prepare Lactococcus lactis culture medium according to Table 1 and adjust the pH to 6.8. If it is a solid medium, add 1.5% agar and sterilize at 115°C for 15 minutes; Dissolve ATCC 11454 dry powder in 0.5mL liquid medium, then insert it into 5mL liquid medium at a ratio of 5% (v / v), and incubate in the incubator for 24 hours; Inoculate on a plate, and culture it statically in an incubator for 20 hours; pick a single colony from the plate, inoculate it in 30 mL of liquid medium, and cultivate it in an incubator for 21 hours. The cultured seed solution was inoculated into fresh 200 mL liquid medium at a ratio of 3% (v / v), and cultured statically in an incubator for 18 hours, and the temperature of the incubator was 31° C.
[0046] (2) Preparation of Nisin suspension: Adjust the pH of the Lactococcus lactis fermentat...
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