A kind of transplanting method of kiwifruit virus-free tissue culture seedling

Abstract

The invention discloses a kiwi fruit detoxification tissue culture seedling transplanting method, relating to the technical field of fruit tree facility cultivation. The method comprises the following steps: (1) tissue culture seedling treating: adjusting the photoperiod of tissue culture seedlings with a seedling age of 3-4 weeks to be 12 hours with illumination and 12 hours in darkness, and meanwhile increasing the illumination intensity during illumination to 3000-5000lux; (2) cultivation seedling preparation: after treating the tissue culture seedlings for 10-15 days, taking out the tissue culture seedlings in a tissue culture bottle, and flushing a stem base culture medium with clear water, until the stem base culture medium is cleaned thoroughly; soaking stem bases into rooting solution, uniformly spraying water on leaf surfaces, and keeping stem leaves wet; (3) transplanting; (4) outdoor seedling hardening. According to the kiwi fruit detoxification tissue culture seedling transplanting method, the illumination time in a later rapid propagation period of the tissue culture seedlings is increased, the illumination intensity is enhanced, the plant stress resistance is enhanced, and the survival rate is ensured; a hole transplanting link is omitted, and manpower and material resources are saved; greenhouse facilities are adopted for cultivation, and the quality of the kiwi fruit tissue culture seedlings is ensured; a soil-free cultivation mode is adopted, the soil-borne disease propagation is reduced, and the nutrition requirements in a kiwi fruit seedling period are met.

Description

technical field [0001] The invention relates to the technical field of facility cultivation of fruit trees, in particular to a method for transplanting kiwi fruit virus-free tissue-cultured seedlings. Background technique [0002] Kiwifruit (Lycium chinense) is a plant of the genus Actinidia in the family Actinidia. It is rich in kiwifruit proteoglycan, VC, phosphorus, iron and other nutrients. At present, kiwifruit propagation techniques are mostly cutting and grafting in production, which can retain the excellent traits of the mother, but also retain the plant viruses in the mother; the accumulation of viruses in kiwifruit increases from generation to generation, and viral diseases continue to affect the yield of kiwifruit. Fruit quality is declining year by year. [0003] Existing technology The rapid propagation of kiwifruit detoxification tissue culture is divided into five stages: (1) obtaining sterile detoxification materials; (2) primary culture of kiwifruit tissue ...

AI Technical Summary

Problems solved by technology

[0004] 1. Kiwi fruit tissue culture seedlings grow indoors for a short time, the plants themselves are not strong enough, and their stress resistance is poor;

[0005] 2. Kiwi fruit tissue culture seedlings take root slowly, and are easily damaged in the later transplanting process. After transplanting, the rooting speed and the ratio of regenerated roots are low, resulting in a low survival rate after transplanting;

[0006] 3. The transplanting environment of kiwifruit tissue culture seedlings is simple, and there is no isolation treatment from the outside world, and the tissue culture seedlings are easy to be repeatedly infected with the virus;

[0007] 4. After the tissue-cultured seedlings have been domesticated and transplanted and survived, they must be artificially planted before sales. This process consumes manpower and material resources, and the survival rate of the plants after artificial planting is low.

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