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Peptide with capability of inhibiting cancer cell growth activity and application thereof

A cancer cell and lung cancer cell technology, applied in the field of medicine and biology, can solve problems such as toxicity, low specificity, and easy drug resistance

Inactive Publication Date: 2016-11-09
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemotherapy drugs have made great progress in the treatment of cancer in recent decades, there are still many problems with current chemotherapy drugs, such as existing toxicity, low specificity, and easy drug resistance. Research Hotspots

Method used

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  • Peptide with capability of inhibiting cancer cell growth activity and application thereof
  • Peptide with capability of inhibiting cancer cell growth activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Synthesis of the peptide of the present invention:

[0016] The present invention adopts the method of chemical synthesis to prepare the peptide, that is, the well-known and mature solid-phase peptide synthesis method in the art, which can adopt either the Boc method or the Fmoc method. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0017] Specifically, use the ABI433A peptide synthesizer of Perkin-Elmer Company, select the Fmoc / HOBt / DCC0.10mmol mode, and use the peptide solid-phase synthesis method to synthesize active peptide monomers and amidated peptide chains. The amino acids used in the synthesis are used Fmoc protection, synthetic resin is HMP resin. After the synthesis, the peptide was cleaved from the resin with a cleavage reagent containing 0.70 g of crystalline phenol, 0.25 ml...

Embodiment 2

[0019] Tests against cancer cells:

[0020] The purpose of this example is to test the effect of the peptide on a series of tumor cells, and the experiment will use physiological saline as a negative control. The selected cell line is A549 (ATCC, U.S.A, catalog number: CCL-185), Hela, human osteoblastoma cell line U-2OS (P53+, RB-; ATCC, U.S.A, catalog number: HTB-96), at 24 1 × 10 5 For swollen cells, the culture medium was 10% FBS DMEM. The next day, the liquid was sucked off, and the peptide or physiological saline prepared in Example 1 was added according to the amount of 0.005 mg / ml, and the liquid was shaken gently for 3 times. 2 Incubate for 180 minutes. Add DMEM medium containing 1% FBS to 1ml, place the cells in 37°C, 5% CO2 and incubate for 3-10 days to observe the state of the cells.

[0021] The experimental results are shown in Table 1 below:

[0022] Table 1 Peptide inhibits the experimental results of tumor cell growth

[0023] group

[0024] It c...

Embodiment 3

[0026] Experiments of inhibiting tumors in mice:

[0027] Take the HepG2 cell line in the logarithmic growth phase and prepare 1×10 cells under sterile conditions. 7 / ml cell suspension, inoculated subcutaneously in the right armpit of nude mice with 0.1ml. The diameter of the transplanted tumor in nude mice was measured with a vernier caliper, and the animals were randomly divided into groups after the tumor grew to 100-200 mm3. Using the method of measuring tumor diameter, dynamically observe the anti-tumor effect of the tested polypeptide. The frequency of tumor diameter measurement was 1 time per day. The method of administration was by tail vein injection. The negative control group was injected with the same amount of normal saline, once a day; the Endostar group was given 3.0 mg / kg, once a day; Dosage is given once a day. After the experiment, the mice were sacrificed, and the tumor mass was removed and weighed. The results are shown in Table 2 below:

[0028] Ta...

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Abstract

The invention relates to a peptide with capability of inhibiting cancer cell growth activity and application thereof and belongs to the field of bio-pharmaceuticals. The peptide comprises the following amino acid sequence: EDYHWFWLWYQQCPK, and the encoding gene of the peptide contains a nucleotide sequence of gaagattatcattggttttggctgtggtatcagcagtgcccgaaa. The peptide has an effect of inhibiting the cancer cell growth activity and especially has activity of inhibiting growth of lung carcinoma cells or cervix uterus cancer cells.

Description

technical field [0001] The invention belongs to the field of medicine and biology, and more specifically relates to a peptide with the activity of inhibiting the growth of cancer cells and its application. Background technique [0002] Chemotherapy has always been the main part in the process of cancer occurrence and treatment. The existing clinical chemotherapeutic drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin and vincristine, etc., are mainly used to destroy the cell cycle, and RNA, DNA and protein are all of them. target of drugs. In recent years, As2O3 has made great progress in the treatment of leukemia. However, this drug and other chemotherapy drugs have their own toxicity, whether one chemotherapy drug is used alone or several chemotherapy drugs are used in combination. In terms of clinical treatment, it has obvious curative effect on cancer, while killing and killing cancer cells, it will also damage the patient's normal cells. The most comm...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/11A61K38/10A61P35/00
CPCC07K7/08A61K38/00
Inventor 王艳玮施放王萍萍孙明周东超杨凯孙大鹏
Owner QINGDAO UNIV
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