Microbial pesticide composition containing amide fungicides and biocontrol bacteria, preparation and application of preparation

Abstract

The invention discloses microbial pesticide composition containing amide fungicides and biocontrol bacteria, a preparation and an application of the preparation and belongs to the field of plant protection and pesticide science. The microbial pesticide composition is prepared from the amide fungicides (in particular to carboxylic acid amide fungicides) and the biocontrol bacterium powder in the mass ratio being (1-10): (1-10), wherein the number of live bacteria in the bacterium powder is not smaller than 106 cfu / g; preferably, the biocontrol bacteria are bacillus amyloliquefaciens ZY-9-13 with the preservation number of CGMCC No.6162. The microbial pesticide composition has excellent control efficacy for tobacco root-knot nematode diseases and tobacco black shank, has the control effect remarkably superior to that of a single chemical fungicide or bacillus amyloliquefaciens biological agent, can delay the fungicide resistance of a chemical agent and is particularly suitable for control on tobacco black shank in the field.

Description

technical field [0001] The invention relates to a fungicide composition containing an amide fungicide and biocontrol bacteria, and also relates to a preparation containing the fungicide composition and its application, belonging to the fields of plant protection and pesticide science. Background technique [0002] Tobacco black shank (Tobacco black shank) is a worldwide devastating soil-borne disease, and its pathogen is Phytophthora parasitica var nicotianae (Breda de Hann) Tuker]. Phytophthora nicotianae can infect tobacco plants from the seedling stage to the adult plant stage, mainly infecting tobacco stems, and can also infect leaves. Tobacco black shank forms water-soaked black spots at the base of the stem, the cortical tissue becomes black and depressed, and spreads up and down along the stem, and the pith is dark brown and necrotic and shrinks into a disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, et al. .Tobacco black shank early prevention technology[J].Plant D...

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Problems solved by technology

For example, the combination of yellow blue-shaped bacteria and manganese zinc can control wheat scab (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. Fermentation conditions of biocontrol strain MT-06 and the control effect of compound fungicides on wheat scab[ J]. Zhejiang Agricultural Sciences, 2013 (07): 821-824), the combination of Bacillus subtilis and tebuconazole can prevent and treat broad bean wilt (Chen Zhiyi, Ren Haiying, Liu Yongfeng, etc. Tebuconazole and Bacillus subtilis synergistically control broad bean Fusarium wilt and its synergistic mechanism [J]. Journal of Pesticide Science, 2002 (4): 40-44), Bacillus subtilis, difenoconazole, azoxystrobin, etc. can control apple ring spot (Zhou Han .Coordinated control of postharvest ring blight by fungicides and biocontrol bacteria and its mechanism[D].Anhui Agricultural University, 2009), Jinggangmycin and Bacillus subtilis synergistically control rice sheath blight (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jinggangmycin and biocontrol bacteria Bs-916 synergistic disease control and synergistic mechanism[J]. Plant Protection Journal, 2003, 30(4):429-434.), etc., but the control target of the combination of the above bacteria and drugs Phytophthora nicotae

The invention patent with the publication number CN104381303A discloses a fungal drug composition for preventing and treating fungal diseases, which is composed of riboflavin and Bacillus subtilis Tpb55 bacterial powder according to the mass ratio of 1.21~2.34:97.96~98.79. Applying Bacillus subtilis Tpb55 bacterial powder, the bacterial drug composition can significantly improve the control effect of tobacco black shank, red spot disease and other diseases, and enhance the disease resistance of plants, but it can only achieve the same control effect as chemical agents

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