Cancer prevention and treatment glucosan and yeast cell wall extract for oral administration
A technology of yeast cell wall and glucan, which is applied in the field of medicine and health care to achieve the effect of repairing human immunity
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Embodiment 1
[0043] A yeast cell wall extract, the preparation method of which is:
[0044] 1) Using a conventional method to break the yeast cell wall and remove the cell content to obtain the yeast cell wall;
[0045] 2) Add 2-5% sodium hydroxide solution to the yeast cell wall, disperse, react at a temperature range of 70-90°C for 45 minutes to 3 hours, and remove the supernatant after centrifugation;
[0046] 3) Repeat step 2) 3 to 5 times;
[0047] 4) Titrate the pH of the product in step 3) to a range of 4.0 to 5.0 with a strong acid, redisperse in a sodium hydroxide solution, react at 70° C. for 45 minutes, and centrifuge to obtain a solid component.
[0048] 5) Washing and purifying with distilled water, and drying to obtain yeast cell wall extract powder, the purity of dextran is 81.46% as detected by high performance liquid chromatography.
[0049] The obtained yeast cell wall extract is displayed by a 9500-fold electron microscope as shown in the figure figure 2 As shown, th...
Embodiment 2
[0052] A yeast cell wall extract, the preparation method of which is:
[0053] 1) Using a conventional method to break the yeast cell wall and remove the cell content to obtain the yeast cell wall;
[0054] 2) Add 2-5% sodium hydroxide solution to the yeast cell wall, disperse, react at a temperature range of 70-90°C for 45 minutes to 3 hours, and remove the supernatant after centrifugation;
[0055] 3) Repeat step 2) 5 to 10 times;
[0056] 4) Titrate the pH of the product in step 3) to a range of 4.0 to 5.0 with a strong acid, redisperse in a sodium hydroxide solution, react at 70° C. for 45 minutes, and centrifuge to obtain a solid component.
[0057] 5) Washing and purifying with distilled water, and drying to obtain yeast cell wall extract powder, the purity of dextran is 93.54% as detected by high performance liquid chromatography.
[0058]The obtained yeast cell wall extract is displayed by a 9500-fold electron microscope as shown in the figure figure 2 As shown, th...
Embodiment 3
[0060] Purchase two commercially available β-1,3 / 1,6-glucans, and the β-1,3 / 1,6-glucans of Example 1 and Example 2 of the present invention, under a 9500X electron microscope Under the observation, the image results are as follows image 3 As shown, its function is shown in the following table:
[0061] Table 1 Molecular structure and anticancer effect contrast of dextran of the present invention and commercially available dextran
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